【摘要】：豬瘟、豬繁殖與呼吸綜合征(藍耳病)和豬偽狂犬病是當前嚴重危害我國生豬生產(chǎn)的病毒性疾病,給我國養(yǎng)豬業(yè)造成了巨大的損失。疫苗免疫是防控這三種疾病的主要手段。為了了解江西省豬瘟、豬繁殖與呼吸綜合征和偽狂犬三種病毒性疾病的疫苗在豬群中的免疫/感染效果,本研究應用酶聯(lián)免疫吸附試驗(ELISA)方法,對2014年1月至2016年12月江西省南昌市等六個地區(qū)231個豬場的5736份血液進行了豬瘟、藍耳、偽狂犬病毒g B(中和)和g E(野毒)抗體檢測。研究結(jié)果表明2014至2016年,豬瘟、藍耳、偽狂犬病毒g B和g E抗體陽性率分別為78.50%、80.82%、85.72%和19.55%。2014至2016年,豬瘟抗體陽性率分別為71.68%、78.45%和82.53%;藍耳抗體陽性率分別為76.68%、81.25%和84.34%;偽狂犬g B抗體分別為80.39%、85.35%和89.92%;偽狂犬g E抗體分別為18.49%、21.97%和24.15%。從檢測結(jié)果可以看出,江西省豬群中豬瘟、藍耳、偽狂犬病3種病毒抗體陽性率符合國家強制性免疫疫苗抗體水平的標準;豬瘟、藍耳和偽狂犬g B抗體陽性率呈逐年遞增的趨勢,然而,偽狂犬野毒感染的比例也呈逐年升高的趨勢。從2014至2016年,各月份間豬瘟、藍耳、偽狂犬野毒中和(g B)和(g E)抗體陽性率變化規(guī)律不明顯。不同階段豬群抗體陽性率差異較大:2014-2016年間,母豬、哺乳仔豬、保育豬和育肥豬藍耳抗體陽性率分別為88.66%、73.92%、60.54%和84.24%;豬瘟病毒抗體陽性率分別為80.01%、72.37%、74.32%和66.39%;偽狂犬病毒中和抗體陽性率分別為90.91%、79.19%、73.05%和82.17%;偽狂犬野毒抗體陽性率分別為11.87%、12.84%、27.08和41.10%。結(jié)果表明,母豬三種病毒抗體陽性率達到了國家強制性免疫疫苗抗體陽性標準(70%),部分保育仔豬和哺乳仔豬藍耳抗體未達標,育肥豬2014和2015年豬瘟抗體陽性率均未達標;各階段豬群的偽狂犬野毒陽性率呈逐年增加的趨勢,其中育肥豬感染尤其嚴重,平均陽性率超過40%。本研究系統(tǒng)地調(diào)查了江西省2014-2016年豬群豬瘟、藍耳、偽狂犬中和(g B)和野毒(g E)抗體水平,在一定程度上揭示了我省豬群抗體水平的整體情況,研究結(jié)果將為豬群疫苗免疫,免疫程序的制定以及三種疫病的監(jiān)測提供了實驗依據(jù)。
[Abstract]:Swine fever, pig reproductive and respiratory syndrome (blue ear disease) and pig pseudorabies are viral diseases that seriously harm the production of live pigs in China. Vaccine immunization is the main means of prevention and control of these three diseases. In order to understand the immune / infection effect of three kinds of viral diseases vaccine of swine fever, porcine reproductive and respiratory syndrome and pseudorabies in Jiangxi province, the (ELISA) method of enzyme linked immunosorbent assay (Elisa) was used in this study. From January 2014 to December 2016, 5736 blood samples from 231 pig farms in Nanchang City, Jiangxi Province, were tested for antibodies against swine fever, blue ear, pseudorabies virus (g B (neutralization) and g E (wild virus. The results showed that from 2014 to 2016, the positive rates of antibody against swine fever, blue ear, pseudorabies virus (GB) and pseudorabies virus (GE) were 78. 50% and 88. 82% respectively, and 19. 55% from 2014 to 2016, respectively. The positive rate of antibody against swine fever was 78.45% and 82.53, the positive rate of blue ear antibody was 76.68% and 84.34%, the positive rate of pseudorabies g B antibody was 80.395.35% and 89.92%, and the positive rate of pseudorabies g E antibody was 18.4921.97% and 24.15%, respectively. From the test results, we can see that the positive rate of antibodies against swine fever, blue ear and pseudorabies in Jiangxi pig herd conforms to the national standard of antibody level of compulsory immunization vaccine. The positive rate of antibody against g-B in blue ear and pseudorabies showed an increasing trend year by year, however, the proportion of pseudorabies virus infection was also increasing year by year. From 2014 to 2016, the positive rates of (g B) and (g E) antibodies against swine fever, blue ear and pseudorabies virus were not obvious. The positive rates of antibody in different stages of pigs were significantly different between 2014 and 2016, sows, suckling piglets, and suckling piglets. The positive rates of blue ear antibody in preserved pigs and fattening pigs were 88.66 / 73.922and 84.24%, respectively; the positive rates of swine fever virus antibody were 80.01and 72.373.32% and 66.39 respectively; the positive rates of pseudorabies virus neutralizing antibody were 90.9179 / 19 / 73.05% and 82.17 respectively; the positive rates of pseudorabies virus antibody were 11.8784 / 27.08 and 41.105respectively. The results showed that the positive rate of three kinds of virus antibodies in sows reached the national standard of compulsory immune vaccine antibody (70%), the blue ear antibodies of some preserved piglets and suckling piglets were not up to the standard, and the positive rates of swine fever antibodies in fattening pigs in 2014 and 2015 were not up to the standard. The positive rate of pseudorabies wild virus was increasing year by year, especially in fattening pigs, the average positive rate was more than 40. In this study, the antibody levels of swine fever, blue ear, pseudorabies neutralizing (g B) and wild virus (g E) from 2014-2016 in Jiangxi Province were systematically investigated, and the whole situation of antibody level of swine herd in Jiangxi Province was revealed to a certain extent. The establishment of immune program and the surveillance of three kinds of epidemic diseases provide experimental basis.
【學位授予單位】：江西農(nóng)業(yè)大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：S858.28

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