【摘要】：非轉(zhuǎn)移性黑色素瘤糖蛋白b(glycoprotein non-metastatic melanoma protein b,GPNMB)及其同系物色素細(xì)胞特異性黑色素細(xì)胞蛋白(pigment cell-specific melanocyte protein,PMEL)對(duì)黑素體的形成和黑色素的生成具有重要的作用,而GPNMB和PMEL的功能還有待深入研究。本文旨在探索GPNMB和PMEL在不同毛色綿羊皮膚表達(dá)是否存在差異,確定紫外線b(UVB)對(duì)GPNMB和PMEL是否有影響。免疫組織化學(xué)結(jié)果表明,GPNMB在不同毛色綿羊皮膚的毛基質(zhì)、內(nèi)外毛根鞘、毛乳頭等區(qū)域均有表達(dá)。Western印跡和qRT-PCR結(jié)果顯示,黑色綿羊皮膚中GPNMB和PMEL表達(dá)量,高于白色綿羊皮膚。黑色素細(xì)胞經(jīng)UVB照射后,GPNMB和PMEL的表達(dá)增高。UVB照射劑量為100 m J/cm2時(shí),黑色素細(xì)胞活性最高;單次UVB輻射后,GPNMB和PMEL mRNA表達(dá)量在72h達(dá)到頂峰,兩者的曲線變化趨勢(shì)大致相同。UVB連續(xù)輻射后,GPNMB mRNA表達(dá)量在4 d達(dá)到頂峰,而PMEL mRNA表達(dá)量雖然在3 d升高,但且對(duì)GPNMB作用更明顯。上述結(jié)果提示,GPNMB和PMEL在不同毛色綿羊皮膚存在差異性表達(dá)。UVB單次或者連續(xù)照射,均促進(jìn)GPNMB和PMEL表達(dá),且對(duì)GPNMB影響更顯著。GPNMB和PMEL可能通過(guò)影響黑素體的成熟進(jìn)而調(diào)節(jié)黑色素的生成。
[Abstract]:The non-metastatic melanoma glycoprotein (b (glycoprotein non-metastatic melanoma protein) and its congener pigment cell-specific melanocyte protein (pigment cell-specific melanocyte) play an important role in the formation of melanoma and the formation of melanin, but the functions of GPNMB and PMEL need to be further studied. The purpose of this study was to explore the difference of GPNMB and PMEL expression in sheep skin with different wool colors and to determine whether UV b (UVB) had an effect on GPNMB and PMEL. The results of immunohistochemistry showed that the expression of GPNMB and PMEL in sheep skin was higher than that in white sheep skin. Western blot and qRT-PCR showed that GPNMB and PMEL were expressed in hair matrix, inner and outer hair root sheath, hair papilla and other areas of sheep skin with different colors of wool. The results of immunohistochemistry showed that the expression of GPNMB and PMEL in black sheep skin was higher than that in white sheep skin. The expression of GPNMB and PMEL in melanocytes increased after UVB irradiation. When the dose of UVB was 100m J/cm2, the activity of melanocytes was the highest, and the expression of GPNMB and PMEL mRNA reached its peak at 72 h after single UVB irradiation. The change trend of the two curves was approximately the same. The expression of GPNMB mRNA reached its peak at 4 days after continuous radiation of UVB, while the expression of PMEL mRNA increased at 3 days, but the effect on GPNMB was more obvious. These results suggest that there are different expressions of GPNMB and PMEL in sheep skin with different color. UVB can promote the expression of GPNMB and PMEL in sheep skin by single or continuous irradiation. The effect on GPNMB is more significant. GPNMB and PMEL may regulate melanin production by affecting the maturation of melanoma.
【作者單位】： 山西農(nóng)業(yè)大學(xué)動(dòng)物科技學(xué)院;河北省深州市職業(yè)技術(shù)教育中心;
【基金】：國(guó)家高技術(shù)研究發(fā)展計(jì)劃項(xiàng)目(863計(jì)劃,No.2013AA102506) 公益性行業(yè)(農(nóng)業(yè))科研專項(xiàng)(No.201303119) 山西農(nóng)業(yè)大學(xué)創(chuàng)新團(tuán)隊(duì)建設(shè)計(jì)劃項(xiàng)目(No.CXTD201201) 山西省研究生教育創(chuàng)新項(xiàng)目(No.2016BY071)資助~~
【分類號(hào)】：S826

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