發(fā)布時間：2018-08-21 15:04

【摘要】：為了解江蘇省雞源結(jié)腸彎曲菌的流行情況和藥物敏感性,對江蘇省不同規(guī)模化養(yǎng)雞場、農(nóng)貿(mào)市場和超市的雞泄殖腔樣品和雞肉產(chǎn)品進行結(jié)腸彎曲菌的分離及PCR鑒定;采用紙片擴散法測定分離株對17種抗菌藥物的敏感性。結(jié)果顯示,從750份樣品(禽肉產(chǎn)品和泄殖腔棉拭子)中分離到81株結(jié)腸彎曲菌,分離率為10.8%。耐藥性試驗結(jié)果顯示,80%以上菌株對頭孢曲松、妥布霉素、諾氟沙星、環(huán)丙沙星、林可霉素和甲氧芐氨嘧啶耐藥,其中甲氧芐氨嘧啶耐藥率達99%,對美羅培南和氟苯尼考保持高度敏感。所有測試菌株出現(xiàn)多重耐藥,優(yōu)勢耐藥譜為LIN/CRO/CN/T/NOR/CIP。調(diào)查結(jié)果為結(jié)腸彎曲菌防控提供參考依據(jù)。
[Abstract]:In order to understand the prevalence and drug sensitivity of Campylobacter coli from chickens in Jiangsu Province, the samples of chicken cloacal and chicken products from different scale chicken farms, farmers' markets and supermarkets in Jiangsu Province were isolated and identified by PCR. The susceptibility of the isolates to 17 antimicrobial agents was determined by disk diffusion method. The results showed that 81 strains of Campylobacter colon were isolated from 750 samples (poultry meat products and cloacal cotton swabs) and the isolation rate was 10.8%. Drug resistance test showed that more than 80% of the strains were resistant to ceftriaxone, tobramycin, norfloxacin, ciprofloxacin, lincomycin and trimethoprim. The resistance rate of trimethoprim was 99% and was highly sensitive to meropenem and florfenicol. All tested strains showed multidrug resistance, and the dominant drug resistance spectrum was LINP / CRO / CN / T / NORP / CIP. The results provide a reference for the prevention and control of Campylobacter colonic.
【作者單位】： 中國農(nóng)業(yè)科學院家禽研究所/農(nóng)業(yè)部禽類產(chǎn)品質(zhì)量安全風險評估實驗室(揚州);
【基金】：十三五國家重點研發(fā)計劃高危金屬中毒病防控技術(shù)研究項目(2016YFD0501208) 2017國家農(nóng)產(chǎn)品質(zhì)量安全風險評估重大專項項目(GJFP2017007) 揚州市社會發(fā)展項目(yz2016058) 揚州市家禽質(zhì)量安全科技服務(wù)平臺建設(shè)項目(yz2015162)
【分類號】：S858.31
，

本文編號：2196113