【摘要】：為確定安徽肥東某山羊場發(fā)生的疑似羊口瘡(ORF)的病原,利用MDBK細(xì)胞從送檢的病羊唇部痂皮中分離獲得病毒,通過PCR鑒定確定病原為羊口瘡病毒(ORFV),并將其命名為ORFV/AH-FD/2016/China株。然后對分離株的B2L基因和F1L基因進(jìn)行克隆、測序,并與其他ORFV序列進(jìn)行遺傳進(jìn)化分析。結(jié)果表明:ORFV/AH-FD/2016/China株的B2L基因長1 137bp,編碼279個氨基酸,F1L基因長1 023bp,編碼341個氨基酸。與GenBank中收錄的其他ORFV流行株相比,B2L基因的核苷酸同源性為96.9%~99.5%,推導(dǎo)的氨基酸同源性為95.5%~98.9%;F1L基因的核苷酸同源性為95.3%~98.3%,推導(dǎo)的氨基酸同源性為94.9%~99.1%。利用軟件構(gòu)建系統(tǒng)進(jìn)化樹,根據(jù)B2L基因遺傳進(jìn)化分析結(jié)果表明,該分離株與甘肅分離株的親緣關(guān)系最近;根據(jù)F1L基因遺傳進(jìn)化分析結(jié)果表明,該分離株與福建分離株親緣關(guān)系最近�；贐2L基因和F1L基因的遺傳進(jìn)化分析,其分離得到的病毒株與國內(nèi)不同地區(qū)分離毒株的遺傳差異不大。該研究為ORFV的后續(xù)研究提供了臨床試驗(yàn)材料和流行病學(xué)依據(jù)。
[Abstract]:In order to determine the pathogen of suspected (ORF) in a goat farm in Feidong, Anhui Province, the virus was isolated from the scab skin of infected sheep lip by MDBK cells. The pathogen was identified as (ORFV), by PCR and named ORFV/AH-FD/2016/China strain. The B2L and F1L genes were cloned and sequenced, and the genetic evolution of B2L and F1L genes were analyzed with other ORFV sequences. The results showed that the B2L gene of the 10: ORFV / AH-FDR / 2016 / China strain was 1137 BP long, and the encoding 279 amino acid F1L gene was 1 023 BP long and 341 amino acids. The nucleotide homology of B2L gene and amino acid homology of F1L gene were 96.9and 99.5, respectively, and the deduced amino acid homology were 95.3398.3and 94.9m / 99.1, respectively, compared with the other ORFV epidemic strains included in GenBank. The nucleotide homology of B2L gene was 96.9% and 99.1% respectively, and the deduced amino acid homology was 95.9% (99.1%), while the nucleotide homology of F1L gene was 95.33.3%. The phylogenetic tree was constructed by software. According to the genetic evolution analysis of B2L gene, the genetic relationship between this isolate and Gansu isolate was the closest, and the result of F1L gene genetic evolution analysis showed that, The relationship between this isolate and Fujian isolate was close. Based on the genetic evolution analysis of B2L gene and F1L gene, the genetic difference between the virus strains isolated from B2L and F1L gene in different regions of China was not significant. This study provides clinical trial materials and epidemiological evidence for the follow-up study of ORFV.
【作者單位】： 安徽農(nóng)業(yè)大學(xué)動物科技學(xué)院;寧波市疾病預(yù)防控制中心;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(31602063) 安徽省自然科學(xué)基金資助項(xiàng)目(1508085QC60) 安徽農(nóng)業(yè)大學(xué)穩(wěn)定和引進(jìn)人才科研項(xiàng)目(yj2015-16);安徽農(nóng)業(yè)大學(xué)大學(xué)生科技創(chuàng)新基金項(xiàng)目(2016-2017)
【分類號】：S852.654

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