【摘要】：口蹄疫和禽流感是我國(guó)的重大動(dòng)物傳染病,長(zhǎng)期影響我國(guó)的畜牧業(yè)的健康發(fā)展。近年來(lái)我區(qū)對(duì)此二病采取疫苗接種為主的綜合性防控措施。為掌握此二病的免疫水平及自然感染狀況,本課題對(duì)2014年度內(nèi)蒙14個(gè)地市進(jìn)行了口蹄疫免疫抗體監(jiān)測(cè)、口蹄疫3ABC抗體檢測(cè)、禽流感免疫抗體監(jiān)測(cè)及禽流感病原學(xué)檢測(cè)。共采集用于口蹄疫抗體檢測(cè)的牛血清樣品1217份、羊血清樣品1914份、豬血清樣品1351份。結(jié)果表明,全區(qū)0型口蹄疫、A型口蹄疫及Asia I口蹄疫的免疫抗體檢測(cè)合格率分別為87.8%、94.4%和87.4%；大多數(shù)盟市免疫效果良好,少數(shù)盟市抗體合格率低于國(guó)家標(biāo)準(zhǔn)；規(guī)模化養(yǎng)殖場(chǎng)免疫效果略好于散養(yǎng)戶；牛的免疫效果略好于羊和豬；牛和羊的3ABC非結(jié)構(gòu)蛋白抗體陽(yáng)性率分別為37.72%和15.99%,揭示我區(qū)口蹄疫自然感染比較普遍。共采集雞血清樣品2653份、口腔和泄殖腔雙拭子樣品2653份。結(jié)果表明,禽流感H5型免疫抗體檢測(cè)合格率為91.71%；大多數(shù)盟市免疫效果良好,只有一個(gè)盟抗體合格率低于國(guó)家標(biāo)準(zhǔn)；規(guī)模化養(yǎng)殖場(chǎng)免疫效果略好于散養(yǎng)戶；禽流感H5和H7亞型病原學(xué)檢測(cè)陽(yáng)性率為0.00%。本研究成果將為我區(qū)口蹄疫和禽流感的防控提供科學(xué)參考。
[Abstract]:Foot-and-mouth disease (FMD) and avian influenza (AIV) are major animal infectious diseases in China, which affect the healthy development of animal husbandry in China for a long time. In recent years, the two diseases in our region to take vaccination-based comprehensive prevention and control measures. In order to understand the immune level and natural infection status of the two diseases, the immune antibody, 3ABC antibody, avian influenza antibody and avian influenza etiology of foot-and-mouth disease (FMD) were detected in 14 cities of Inner Mongolia in 2014. A total of 1217 bovine serum samples, 1914 sheep serum samples and 1351 pig serum samples were collected for FMD antibody detection. The results showed that the qualified rates of immune antibody detection of foot-and-mouth disease type A and Asia I were 87.894% and 87.4%, respectively. The immunological effect of large scale farm was better than that of bulk family, the immune effect of cattle was better than that of sheep and pig, and the positive rates of 3ABC non-structural protein antibody in cattle and sheep were 37.72% and 15.99%, respectively, which revealed that the natural infection of foot-and-mouth disease was common in our area. A total of 2653 chicken serum samples and 2653 oral and cloacal double swabs were collected. The results showed that the qualified rate of H5 antibody detection was 91.71%, the immune effect was good in most of the cities, only one league antibody qualified rate was lower than the national standard, and the immune effect of large-scale breeding farm was slightly better than that of free-range farmers. The positive rate of pathogenic detection of avian influenza H 5 and H 7 subtypes was 0.005%. The results of this study will provide a scientific reference for the prevention and control of foot-and-mouth disease and avian influenza in our region.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S852.4

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 楊素娟;;口蹄疫流行病學(xué)及感染機(jī)制的研究進(jìn)展[J];北京農(nóng)業(yè);2007年12期

2 楊新剛;靳月生;姚學(xué)軍;呂艷秋;谷瑞平;王紅;李榮華;王岳偉;于麗娜;邱海蓮;;禽流感血凝抑制試驗(yàn)(HI)的影響因素分析[J];北京農(nóng)業(yè);2009年06期

3 金元昌,李景鵬,張龍,孫岳,于敏,楊甲芳;禽流感病毒分子生物學(xué)研究進(jìn)展[J];動(dòng)物醫(yī)學(xué)進(jìn)展;2003年01期

4 曹梅,田夫林,莊文忠;禽流感病毒血凝素分子生物學(xué)研究進(jìn)展[J];動(dòng)物醫(yī)學(xué)進(jìn)展;2004年02期

5 黃庚明,辛朝安;禽流感病毒血凝素(HA)基因的研究進(jìn)展[J];廣東畜牧獸醫(yī)科技;2001年02期

6 劉俊林;祁淑蕓;馬軍武;崔燕;;液相阻斷ELISA在口蹄疫病毒抗體水平檢測(cè)中的應(yīng)用[J];甘肅農(nóng)業(yè)大學(xué)學(xué)報(bào);2008年03期

7 汪洋,亢文華,白永平,薄清如;口蹄疫研究概況[J];中國(guó)畜牧獸醫(yī);2004年09期

8 黃建龍;朱春霞;劉道新;;我國(guó)低致病性禽流感研究概況[J];動(dòng)物醫(yī)學(xué)進(jìn)展;2014年04期

9 譚偉;徐倩;謝芝勛;;禽流感病毒研究概述[J];基因組學(xué)與應(yīng)用生物學(xué);2014年01期

10 曲百友;王劍;;獸醫(yī)實(shí)驗(yàn)室ELISA檢測(cè)中應(yīng)注意的問(wèn)題[J];山東畜牧獸醫(yī);2014年05期

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