【摘要】：雌激素受體α(estrogen receptor,ERα)基因在動物繁殖過程中起著重要作用,已經(jīng)被認(rèn)為是影響豬(Sus scrofa)產(chǎn)仔數(shù)的主效基因,但在山羊(Capra hicus)方面研究較少。為了揭示雌激素受體基因?qū)F州本地山羊繁殖性狀的影響,本研究采用直接測序技術(shù)檢測了該基因8個外顯子的SNP,并使用qRT-PCR檢測ERα基因在黔北麻羊的下丘腦,垂體,性腺軸中不同表達(dá)量。結(jié)果表明,ERα基因在黔北麻羊和貴州黑山羊第8外顯子中均存在C127T突變,且屬于錯義突變,致使該基因mRNA二級結(jié)構(gòu)以及編碼蛋白結(jié)構(gòu)的改變。在第8外顯子的C127T位點發(fā)現(xiàn)CC、CT和TT 3種基因型,其中,在黔北麻羊群體中CC基因型個體的產(chǎn)羔數(shù)與TT和CT基因型間達(dá)差異顯著水平(P0.05),在貴州黑山羊群體中未達(dá)到顯著差異。qRT-PCR檢測結(jié)果顯示,單羔組和多羔組黔北麻羊的5個組織中ERα基因均有表達(dá),且卵巢均最高。進(jìn)一步研究發(fā)現(xiàn),單羔組和多羔組黔北麻羊卵巢組織中的ERαmRNA表達(dá)量差異顯著(P0.05),而在其他組織間均未達(dá)到差異顯著(P0.05)。結(jié)果顯示,ERα中的C127T突變可能影響貴州地方山羊的產(chǎn)羔數(shù),為培育貴州地方山羊高繁殖力品系提供了理論依據(jù)。
[Abstract]:Estrogen receptor ER 偽 (ER 偽) gene plays an important role in animal reproduction. It has been considered as a major gene affecting piglet (Sus scrofa) litter size, but little research has been done on goat (Capra hicus). In order to reveal the effect of estrogen receptor gene on reproductive traits of Guizhou native goats, the SNPs of 8 exons of the gene were detected by direct sequencing, and the ER 偽 gene was detected by qRT-PCR in the hypothalamus and pituitary of Qianbei Ma sheep. Different levels of expression were found in the gonadal axis. The results showed that there was C127T mutation and missense mutation in exon 8 of Qianbei Ma sheep and Guizhou black goat, which resulted in the change of mRNA secondary structure and coding protein structure. Three genotypes of CCT CT and TT were found at the C127T locus of exon 8, in which, There was significant difference in lambs size among CC genotype individuals and TT and CT genotypes (P0.05), but there was no significant difference in Guizhou black goat population by qRT-PCR. The ER 偽 gene was expressed in 5 tissues of Qianbei Ma sheep in single lambs and many lambs, and the ovary was the highest. Further study showed that the expression of ER 偽 mRNA in ovarian tissues of single lambs group and multiple lambs group was significantly different (P0.05), but there was no significant difference among other tissues (P0.05). The results showed that the C127T mutation in ER 偽 might affect the lambing size of Guizhou local goats, which provided a theoretical basis for breeding Guizhou local goat lines with high fecundity.
【作者單位】： 貴州大學(xué)動物科學(xué)學(xué)院/高原山地遺傳育種與繁殖省部共建教育部重點實驗室/貴州省動物遺傳育種與繁殖重點實驗室;
【基金】：貴州省科技重大專項[黔科合重大專項字2013(6008)] 貴州大學(xué)研究生創(chuàng)新基金(研農(nóng)2016024)
【分類號】：S827

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