【摘要】：促生長(zhǎng)肽是以豬生長(zhǎng)激素單克隆抗體為靶標(biāo),結(jié)合免疫學(xué)和計(jì)算生物學(xué)等相關(guān)技術(shù)篩選出的一種具有模擬豬生長(zhǎng)激素作用的肽類物質(zhì)。目前已經(jīng)在細(xì)胞水平上證明了該物質(zhì)具有與豬生長(zhǎng)激素相似的生物活性和功能,但促生長(zhǎng)肽對(duì)動(dòng)物促生長(zhǎng)的有效性驗(yàn)證尚未進(jìn)行。為觀察促生長(zhǎng)肽與組織器官的動(dòng)態(tài)結(jié)合特性、代謝特性,利用小動(dòng)物活體成像系統(tǒng),活體觀測(cè)大鼠體內(nèi)熒光標(biāo)記的促生長(zhǎng)功能肽和豬生長(zhǎng)激素分布及熒光強(qiáng)度的消長(zhǎng)規(guī)律,剖解觀測(cè)具體結(jié)合部位情況。研究發(fā)現(xiàn),促生長(zhǎng)肽與豬生長(zhǎng)激素均能與大鼠體內(nèi)生長(zhǎng)激素的主要靶器官發(fā)生特異性結(jié)合,兩種物質(zhì)在體內(nèi)結(jié)合的器官和部位相同,但是促生長(zhǎng)肽與組織結(jié)合的強(qiáng)度較弱;兩者在大鼠體內(nèi)的代謝路徑相似,但促生長(zhǎng)肽的代謝速度明顯快于豬生長(zhǎng)激素的現(xiàn)象。為驗(yàn)證促生長(zhǎng)肽對(duì)動(dòng)物促生長(zhǎng)作用,首先利用試驗(yàn)動(dòng)物大鼠進(jìn)行試驗(yàn)方案,注射劑量、注射時(shí)期的選擇等基礎(chǔ)的探索研究。試驗(yàn)數(shù)據(jù)顯示添加促生長(zhǎng)肽能夠調(diào)控體長(zhǎng)增長(zhǎng)效果明顯,表現(xiàn)為動(dòng)物體長(zhǎng)的增長(zhǎng)高峰提前;血液中骨鈣素含量明顯升高(P0.05),堿性磷酸酶有增高趨勢(shì),但差異不顯著(P0.05)。血液中與骨骼生長(zhǎng)密切相關(guān)指標(biāo)的表現(xiàn)提示促生長(zhǎng)肽調(diào)控體長(zhǎng)增長(zhǎng)與促進(jìn)骨骼生長(zhǎng)密切相關(guān)。添加促生長(zhǎng)肽能夠調(diào)控體重的增長(zhǎng),但與使用劑量密切相關(guān),在試驗(yàn)的各個(gè)時(shí)間點(diǎn)觀測(cè)到高中低添加量對(duì)體重增長(zhǎng)的效果不一。與血液數(shù)據(jù)對(duì)比分析,血清總蛋白呈現(xiàn)增高趨勢(shì),IGF-1變化不明顯,二者與對(duì)照組相比差異不顯著(P0.05),而尿素氮顯著低于對(duì)照組(P0.05)。提示促生長(zhǎng)肽調(diào)控體重增長(zhǎng)與促進(jìn)蛋白合成相關(guān)。開展了促生長(zhǎng)肽對(duì)斷奶仔豬的促生長(zhǎng)作用的現(xiàn)場(chǎng)應(yīng)用研究。試驗(yàn)組分高劑量組和低劑量組。試驗(yàn)結(jié)果表明:添加促生長(zhǎng)肽能夠使豬平均日增重呈現(xiàn)提高趨勢(shì),但變化規(guī)律不明顯。表現(xiàn)為高劑量組在前期和末期效果均達(dá)到顯著(P0.05),低劑量組后期效果顯著(P0.05)。血液指標(biāo)顯示低劑量能夠提高仔豬IGF-1的和總蛋白的含量,但差異不顯著(P0.05),促進(jìn)骨鈣素增高,減緩堿性磷酸酶濃度的下降,差異顯著(P0.05)。研究結(jié)果提示:促生長(zhǎng)肽對(duì)豬的生長(zhǎng)具有促進(jìn)作用,能夠促進(jìn)蛋白的合成及骨生長(zhǎng),但內(nèi)在的變化機(jī)理尚需要深入研究。
[Abstract]:Growth promoting peptide (GFP) is a peptide which is screened by monoclonal antibody against porcine growth hormone and combined with immunology and computational biology. At present, it has been proved that the substance has the same biological activity and function as porcine growth hormone at the cell level, but the effectiveness of GFP on animal growth has not been verified. In order to observe the dynamic binding characteristics and metabolic characteristics of growth promoting peptide with tissues and organs, the distribution and intensity of growth promoting peptide and pig growth hormone labeled by fluorescence in vivo were observed by small animal imaging system in vivo. Dissecting and observing the concrete joint position. It was found that the growth promoting peptide and pig growth hormone could bind specifically to the main target organs of growth hormone in rats. The organs and parts of the two substances bound in vivo were the same, but the intensity of the binding between growth peptide and tissue was weak. The metabolic pathways were similar in rats, but the growth promoting peptide metabolism was faster than that of pig growth hormone. In order to verify the growth promoting effect of GFP on animals, the experimental scheme, injection dose and selection of injection time were used to investigate the effects of GFP on animal growth. The experimental data showed that the growth promoting peptide could regulate the growth of body length obviously, showing that the growth peak of animal body length was earlier; the content of osteocalcin in blood increased significantly (P0.05), and alkaline phosphatase increased, but the difference was not significant (P0.05). The expression of blood closely related to bone growth suggests that growth peptide regulates the growth of body length and promotes bone growth. The effect of high, middle and low supplementation on weight gain was observed at each time point in the experiment, but it was closely related to the use dose. Compared with the blood data, the serum total protein showed an increasing trend and IGF-1 did not change significantly, compared with the control group, there was no significant difference (P0.05), but urea nitrogen was significantly lower than the control group (P0.05). It is suggested that the growth promoting peptide is related to the regulation of body weight gain and the promotion of protein synthesis. The field application study of growth promoting peptide on weaning piglets was carried out. The test group was divided into high dose group and low dose group. The results showed that the average daily gain (ADG) of pigs increased with the addition of GFP, but the rule of change was not obvious. The results showed that the effect of high dose group was significant at the early and end stage (P0.05), and that of low dose group was significant at the later stage (P0.05). Blood index showed that low dose could increase the content of IGF-1 and total protein, but the difference was not significant (P0.05), promote the increase of osteocalcin, slow down the decrease of alkaline phosphatase concentration, the difference was significant (P0.05). The results suggest that GFP can promote the growth of pigs and promote the synthesis of protein and bone growth, but the intrinsic mechanism needs further study.
【學(xué)位授予單位】：吉林農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S828.5

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本文編號(hào)：2170168