【摘要】：輸血療法是一種輸入健康動物的血液或血液成分來進行治療的方法。機體血液或血液成分丟失時,輸血療法可以起到補血、止血、解毒的治療效果,作為一種有效的搶救措施已經(jīng)廣泛應(yīng)用在臨床診療之中。異源輸血是異種動物之間的輸血,包括全血、血液成分和血液制品,臨床上已有相關(guān)病例。本文通過對貓異源輸入犬血的可行性研究及其對貓急性失血性休克的療效觀察,進而為其在寵物臨床上應(yīng)用提供指導。試驗Ⅰ貓異源輸入犬血的可行性試驗研究本試驗研究貓異源輸入犬血的可行性。試驗選取18只平均體重(4±0.5)kg的健康成年貓,隨機分為3組,每組6只。采用耳標法對所有試驗貓進行標記識別。分別設(shè)定為空白對照組A組(靜脈輸入生理鹽水)、試驗組B組(靜脈輸入貓全血)、試驗組C組(靜脈輸入犬全血),輸入量為受試動物總血量的20%。試驗期間觀察各組試驗貓的臨床表現(xiàn),比較各組貓在試驗前2h、試驗后0.5 h、1h、2 h及連續(xù)7 d的血液學、血清生化、血清電解質(zhì)、尿液等指標,評價貓異源輸入犬血的可行性。結(jié)果表明:輸入貓全血的B組、輸入犬全血的C組與輸入生理鹽水的A組之間差異不顯著(p0.05)。試驗Ⅱ 貓急性失血性休克模型的建立本試驗選取9只平均體重(4±0.5)kg的健康成年貓,采取自身前后對照方法,心臟放血建立貓急性失血性休克模型。具體方法:將各組試驗貓鎮(zhèn)靜后按20 ml/kg進行心臟放血,并于試驗期間觀察試驗貓臨床癥狀,比較模型制備前30 min(T01)及放血完成后即刻(T0)、15 min(T15)、30 min(T30)四個時間點,試驗貓的精神狀態(tài)、體溫、呼吸、心率、脈搏、可視粘膜顏色、飲欲、食欲、糞便以及尿液情況等。結(jié)果:共有9只貓成功建立急性失血性中度休克模型,各試驗貓失血后出現(xiàn)體溫下降、心率增快、可視黏膜顏色蒼白、精神委靡、糞尿明顯減少等癥狀;與模型制備前30 min(T01)相比,放血完成后30 min(T30),試驗貓淋巴細胞、單核細胞、中性粒細胞、紅細胞、血紅蛋白、紅細胞壓積明顯下降超出正常范圍,且差異顯著(P0.05),Na+、K+、Cl-含量明顯下降(P0.05),血壓明顯降低(P0.05)。結(jié)果表明:以貓作為模型動物,采用心臟放血的方法可以確實、迅速的構(gòu)建急性失血性休克模型。試驗Ⅲ 貓異源輸入犬血對貓急性失血性休克的療效觀察本試驗為探究貓異源輸入犬血對貓急性失血性休克的治療效果,將試驗Ⅱ中急性失血性休克建模成功的9只貓隨機分為3組,分別設(shè)定為輸液組(A)、輸液+貓血組(B)、輸液+犬血組(C)。通過觀察輸液前2h、輸液完成后2h及第1、3、7、14 d各組試驗貓的臨床癥狀、血液學、血清生化、血清電解質(zhì)及尿液等指標,評價貓異源輸入犬血的臨床療效。結(jié)果表明:B、C組的各項指標均與A組有顯著差異(p0.05),B、C組之間差異不顯著(p0.05);試驗期間輸血組未出現(xiàn)與輸血相關(guān)的不良反應(yīng)。表明:貓異源輸入犬血對貓急性失血性休克有療效。
[Abstract]:Blood transfusion therapy is a method of being treated by importing blood or blood components from healthy animals. When blood or blood component is lost, blood transfusion therapy has been widely used in clinical diagnosis and treatment. Heterogenous blood transfusion is a kind of blood transfusion between animals, including whole blood, blood components and blood products. In this paper, the feasibility of xenotransfusion of canine blood from cats and the observation of its curative effect on acute hemorrhagic shock in cats were studied, thus providing guidance for its clinical application in pets. Experimental study on the feasibility of xenogeneic transfusion of canine blood from cats the feasibility of xenogeneic transfusion of canine blood from cats was studied in this experiment. Eighteen healthy adult cats with an average body weight of (4 鹵0.5) kg were randomly divided into 3 groups with 6 cats in each group. All test cats were identified by ear labeling method. Control group A (intravenous infusion of normal saline), test group B (intravenous infusion of whole cat blood), group C (intravenous infusion of whole dog blood), the amount of input is 20% of the total blood volume of the tested animals. During the experiment, the clinical manifestations of the cats in each group were observed, and the hematology, serum biochemistry, serum electrolyte and urine were compared for 2 hours before the experiment, 1 hour after the experiment and 2 hours after the experiment for 7 days, and the feasibility of xenogeneic transfusion of dog blood was evaluated. The results showed that there was no significant difference between group B, group C and group A (p0.05). Establishment of acute hemorrhagic shock model in cats. Nine healthy adult cats with average body weight of (4 鹵0.5) kg were used to establish acute hemorrhagic shock model by cardiac bleeding. Methods: the experimental cats were treated with cardiac bleeding for 20 ml/kg after sedation. The clinical symptoms of the cats were observed during the experiment. The mental state of the experimental cats was compared at 30 min (T01) before the model preparation and 30 min (T30) at 15 min (T15) immediately after bloodletting. Body temperature, respiration, heart rate, pulse, color of visible mucosa, appetite, feces and urine. Results: a total of 9 cats were successfully established acute hemorrhagic moderate shock model. After blood loss, the cats had symptoms such as hypothermia, rapid heart rate, pale visible mucosal color, mental disorder, decreased feces and urine, and so on. Compared with 30 min before model preparation (T01), 30 min after bloodletting (T30), cat lymphocytes, monocytes, neutrophils, erythrocytes, hemoglobin, hematocrit significantly decreased beyond the normal range. The difference was significant (P0.05). The content of Na + K + Cl- was significantly decreased (P0.05), and the blood pressure was significantly decreased (P0.05). The results showed that the model of acute hemorrhagic shock could be established by using the method of cardiac bleeding. Experimental observation on the therapeutic effect of xenotransfusion of canine blood on acute hemorrhagic shock in cats the purpose of this study was to investigate the therapeutic effect of xenotransfusion of canine blood on acute hemorrhagic shock in cats. Nine cats with successful modeling of acute hemorrhagic shock in test 鈪，

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