【摘要】：牦牛(Bos Grunniens)作為高原地區(qū)特有物種,以高寒草地牧草為食,其高寒、低氧的惡劣生活環(huán)境使得腹瀉疾病發(fā)生呈常態(tài)化,尤其是1日齡牦牛腹瀉疾病尤為嚴(yán)重。腸黏膜免疫系統(tǒng)是機(jī)體重要的免疫屏障,腸黏膜免疫相關(guān)細(xì)胞在腸黏膜發(fā)揮免疫效應(yīng)中起著重要的作用。本研究旨在對(duì)1日齡牦牛小腸黏膜免疫相關(guān)細(xì)胞的數(shù)量變化進(jìn)行研究。采用免疫組織化學(xué)和組織學(xué)特殊染色技術(shù),分別對(duì)小腸的上皮內(nèi)淋巴細(xì)胞、固有層淋巴細(xì)胞、上皮內(nèi)杯狀細(xì)胞和肥大細(xì)胞分布特點(diǎn)和數(shù)量變化進(jìn)行研究,對(duì)各腸段分泌的粘液性質(zhì)進(jìn)行判定。研究結(jié)果顯示:上皮內(nèi)淋巴細(xì)胞數(shù)量由十二指腸向空腸、回腸呈依次減少的趨勢(shì),差異極顯著(P0.01);固有層淋巴細(xì)胞數(shù)量、肥大細(xì)胞數(shù)量從十二指腸向回腸呈依次遞增的趨勢(shì),差異極顯著(P0.01);上皮內(nèi)杯狀細(xì)胞從十二指腸到回腸呈增長(zhǎng)趨勢(shì),十二指腸與空腸差異顯著(P0.05),但二者與回腸差異極顯著(p0.01);十二指腸上皮杯狀細(xì)胞分泌中性粘液,而空腸、回腸分泌酸性粘液或中性和酸性混合性粘液;十二指腸腺靠近黏膜層分泌中性粘液,靠近肌層分泌酸性粘液;小腸腺均分泌有中性和酸性混合性粘液。1日齡牦牛小腸不同腸段黏膜相關(guān)免疫細(xì)胞數(shù)量的不同變化,顯示了小腸黏膜為機(jī)體天然屏障的重要組成部分。
[Abstract]:Yak (Bos Grunniens), as a endemic species in plateau area, feeds on herbage in alpine grassland. Its harsh living environment of high cold and low oxygen makes the diarrhea disease normal, especially the diarrhea disease of yak at the age of 1 day. Intestinal mucosal immune system is an important immune barrier, and intestinal mucosal immune-associated cells play an important role in the immune effect of intestinal mucosa. The purpose of this study was to study the changes of the number of mucosal immune-associated cells in the small intestine of 1-day-old yak. The distribution and quantity of intraepithelial lymphocytes, lamina propria lymphocytes, intraepithelial goblet cells and mast cells in the small intestine were studied by immunohistochemical and histological special staining techniques. The properties of mucus secreted by each intestinal segment were determined. The results showed that the number of intraepithelial lymphocytes decreased from duodenum to jejunum and ileum decreased (P0.01), and the number of lymphocytes in lamina propria and mast cell increased from duodenum to ileum. The intraepithelial goblet cells increased from duodenum to ileum, the difference between duodenum and jejunum was significant (P0.05), but the difference between duodenal goblet cells and ileum was very significant (p0.01), duodenal goblet cells secreted neutrophilic mucus, while jejunum, duodenal goblet cells secreted neutrophilic mucus and jejunum. Ileum secreted acidic mucus or mixed neutral and acidic mucus, duodenal gland secreted neutral mucus near mucosal layer and acidic mucus near muscle layer. The intestinal glands secreted neutral and acidic mixed mucus. 1. The number of mucosal associated immune cells in different intestinal segments of yak small intestine showed that intestinal mucosa was an important part of the natural barrier of the body.
【學(xué)位授予單位】：甘肅農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：S823.85

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本文編號(hào)：2153717