3種中藥活性成分對豬圓環(huán)病毒2型疫苗免疫效果的影響
發(fā)布時間：2018-07-29 15:42
【摘要】：為篩選對豬圓環(huán)病毒2型(PCV2)疫苗有顯著免疫增強作用的中藥活性成分,本研究將66頭14日齡豬隨機分為11組,分別為免疫對照組、空白對照組和9個給藥組。飼養(yǎng)至21 d時,除空白對照組外,其余各組注射2 mL PCV2滅活疫苗。在免疫前3 d和免疫后3 d,9個給藥組分別灌喂3個劑量水平(高劑量12 mg,中劑量8 mg,低劑量4 mg)野黃芩苷、苦參堿和黃芪多糖溶液5 mL,免疫對照組和空白對照組灌喂等體積生理鹽水。分別在免疫后第7 d、14 d、21 d和28 d,各組隨機抽取5頭豬靜脈采血,測定PCV2抗體水平、特異性免疫球蛋白IgG含量、外周血CD~(3+)含量和CD4~+/CD8~+值、血清IL-2及IFN-γ含量變化。結(jié)果表明,3種中藥活性成分對PCV2疫苗的免疫效果均有不同程度的增強作用,其中野黃芩苷中劑量組效果最佳,可以作為PCV2疫苗免疫增強劑的候選藥。
[Abstract]:In this study, 66 14-day-old pigs were randomly divided into 11 groups, which were immunized control group, blank control group and 9 drug administration groups, in order to screen the active components of traditional Chinese medicine (TCM) which could significantly enhance the immunity of porcine circovirus type 2 (PCV2) vaccine. After 21 days of feeding, 2 mL PCV2 inactivated vaccine was injected into the other groups except the blank control group. Three days before and 3 days after immunization, the 9 groups were given three doses of baicalin (high dose 12 mg, middle dose 8 mg, low dose 4 mg), respectively. Matrine and astragalus polysaccharide solution were 5 mL, and the immune control group and the blank control group were fed with the same volume of normal saline. On the 7th and 28th days after immunization, 5 pigs were randomly selected for venous blood collection. The levels of PCV2 antibody, the content of specific immunoglobulin (IgG), the contents of CD3 in peripheral blood and CD4 ~ / CD8 ~, the contents of serum IL-2 and IFN- 緯 were measured. The results showed that the three active components of Chinese herbal medicine could enhance the immune effect of PCV2 vaccine to varying degrees, among which the middle dose group of baicalin was the best, and could be used as a candidate for PCV2 vaccine immune enhancer.
【作者單位】： 山西農(nóng)業(yè)大學動物科技學院;江蘇農(nóng)牧科技職業(yè)學院;無錫市農(nóng)業(yè)委員會農(nóng)業(yè)信息服務(wù)中心;
【基金】：西藏自然科學基金(ZJ2013018) 江蘇農(nóng)牧科技職業(yè)學院“鳳凰人才工程” 江蘇省“333工程”(蘇農(nóng)辦人2016[3]號)
【分類號】：S853.7
本文編號：2153159
[Abstract]:In this study, 66 14-day-old pigs were randomly divided into 11 groups, which were immunized control group, blank control group and 9 drug administration groups, in order to screen the active components of traditional Chinese medicine (TCM) which could significantly enhance the immunity of porcine circovirus type 2 (PCV2) vaccine. After 21 days of feeding, 2 mL PCV2 inactivated vaccine was injected into the other groups except the blank control group. Three days before and 3 days after immunization, the 9 groups were given three doses of baicalin (high dose 12 mg, middle dose 8 mg, low dose 4 mg), respectively. Matrine and astragalus polysaccharide solution were 5 mL, and the immune control group and the blank control group were fed with the same volume of normal saline. On the 7th and 28th days after immunization, 5 pigs were randomly selected for venous blood collection. The levels of PCV2 antibody, the content of specific immunoglobulin (IgG), the contents of CD3 in peripheral blood and CD4 ~ / CD8 ~, the contents of serum IL-2 and IFN- 緯 were measured. The results showed that the three active components of Chinese herbal medicine could enhance the immune effect of PCV2 vaccine to varying degrees, among which the middle dose group of baicalin was the best, and could be used as a candidate for PCV2 vaccine immune enhancer.
【作者單位】： 山西農(nóng)業(yè)大學動物科技學院;江蘇農(nóng)牧科技職業(yè)學院;無錫市農(nóng)業(yè)委員會農(nóng)業(yè)信息服務(wù)中心;
【基金】：西藏自然科學基金(ZJ2013018) 江蘇農(nóng)牧科技職業(yè)學院“鳳凰人才工程” 江蘇省“333工程”(蘇農(nóng)辦人2016[3]號)
【分類號】：S853.7
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