【摘要】：為了從豬源大腸桿菌中獲得純度高、產(chǎn)率高的脂多糖(lipopolysaccharides,LPS),并評價其毒力,本試驗采集仔豬腹瀉糞便作為病料,進行細(xì)菌分離鑒定和16SrDNA鑒定,采用熱酚水法提取細(xì)菌的LPS,通過DNaseⅠ、RNaseA、蛋白酶K和醇沉法對LPS進行純化,通過苯酚硫酸法、Bradford法和紫外分光光度法測定LPS多糖、蛋白及核酸含量,鱟試劑定量法測定其活性,采用小鼠急性毒性試驗方法測定小鼠半數(shù)致死量(LD50)。結(jié)果顯示,分離獲得一株具有高致病性的豬源大腸桿菌,純化的豬源大腸桿菌LPS平均產(chǎn)率為3.74%,其中多糖含量為13.40%,蛋白含量為0.48%,核酸含量為0.06%,主要條帶集中在14~160ku范圍內(nèi),鱟試劑定量法測定其活性為1.21×107 EU/mg,提取LPS對小鼠的LD50為31.86mg/kg。該豬場仔豬腹瀉是由致病性大腸桿菌引起,獲得其毒力因子LPS純度高、產(chǎn)率高、毒力強,這將為臨床防制豬大腸桿菌病及LPS提取純化提供理論依據(jù)。
[Abstract]:In order to obtain lipopolysaccharide (LPS) from porcine Escherichia coli with high purity and yield and to evaluate its virulence, the feces of piglet diarrhea were collected as diseased materials, and the bacteria were isolated and identified by 16SrDNA. The LPS was purified by DNase 鈪，

本文編號：2147939