牦牛角性狀候選基因的篩選
發(fā)布時(shí)間：2018-07-25 07:03
【摘要】：本研究旨在篩選牦牛角性狀候選基因,為角發(fā)育機(jī)制的研究和無角牦牛的培育提供科學(xué)依據(jù)。采集牦牛角發(fā)育初期胎齡90d的有角和無角牛的角基及額部皮膚組織,利用實(shí)時(shí)熒光定量PCR技術(shù)(qRT-PCR),比較牛角性狀候選基因OLIG1、OLIG2、IFNAR1、IFNAR2、C1 H21orf62、GCFC1、IFNGR2、SYNJ1、IL10RB、GART、RXFP2、FOXL2、TWIST1、TWIST2、ZEB2、E-cadherin和P-cadherin mRNA在角基間及皮膚間的相對表達(dá)量。結(jié)果顯示,候選基因OLIG1、IFNAR1、C1 H21orf62、SYNJ1、IL10RB、GART、RXFP2、TWIST2和E-cadherin在有角和無角牦牛角基間和皮膚間轉(zhuǎn)錄量差異不顯著(P0.05)。IFNGR2、FOXL2、TWIST1和P-cadherin在有角和無角牦牛角基間轉(zhuǎn)錄量差異顯著(P0.05)。IFNAR2和GCFC1在有角和無角牦牛角基間和皮膚間轉(zhuǎn)錄量差異都顯著(P0.05)。OLIG2和ZEB2在有角和無角牦牛角基間轉(zhuǎn)錄量差異極顯著(P0.01)。推測OLIG2和FOXL2可能是牛角性狀的關(guān)鍵候選基因,同時(shí)ZEB2、TWIST1和IFNGR2基因可能對牛角的形成具有重要作用,而P-cadherin基因可能參與了牛角的發(fā)育。
[Abstract]:The purpose of this study was to screen candidate genes for yak horn traits and provide scientific basis for the study of angular development mechanism and the cultivation of horned yaks. The angular base and forehead skin tissues of horned and horned cattle were collected at the early stage of yak angle development 90d, and the real-time fluorescent quantitative PCR technique (qRT-PCR) was used to compare OLIG1, OLIG2, IFNAR1, IFNAR2, C1 H21orf62, GCFC1, IFNGR2, SYNJ1, IL10RB, GART, RXFP2, FOXL2, TWIST1, TWIST2, etc. The difference of P0.05.IFNGR2, FOXL2, TWIST1 and P-cadherin in the angular and non angular yak horns was significant (P0.05) between.IFNAR2 and GCFC1 in the angular and non angular yak horns and between the skin, and the difference in the transcriptional amount between the yak horns and the skin was significant (P0.05).OLIG2 and ZEB2 (P0.01) between the horns and the horned yak horns (P0.01). FOXL2 may be a key candidate gene for horns, and ZEB2, TWIST1 and IFNGR2 genes may play an important role in the formation of horns, and the P-cadherin gene may be involved in the development of horns.
【作者單位】： 中國農(nóng)業(yè)科學(xué)院蘭州畜牧與獸藥研究所;甘肅省牦牛繁育工程重點(diǎn)實(shí)驗(yàn)室;
【基金】：牦牛遺傳資源與育種(CAAS-ASTIP-2014-LIHPS-01) 科技部科技支撐計(jì)劃(2012BAD13B05)
【分類號】：S823.85
[Abstract]:The purpose of this study was to screen candidate genes for yak horn traits and provide scientific basis for the study of angular development mechanism and the cultivation of horned yaks. The angular base and forehead skin tissues of horned and horned cattle were collected at the early stage of yak angle development 90d, and the real-time fluorescent quantitative PCR technique (qRT-PCR) was used to compare OLIG1, OLIG2, IFNAR1, IFNAR2, C1 H21orf62, GCFC1, IFNGR2, SYNJ1, IL10RB, GART, RXFP2, FOXL2, TWIST1, TWIST2, etc. The difference of P0.05.IFNGR2, FOXL2, TWIST1 and P-cadherin in the angular and non angular yak horns was significant (P0.05) between.IFNAR2 and GCFC1 in the angular and non angular yak horns and between the skin, and the difference in the transcriptional amount between the yak horns and the skin was significant (P0.05).OLIG2 and ZEB2 (P0.01) between the horns and the horned yak horns (P0.01). FOXL2 may be a key candidate gene for horns, and ZEB2, TWIST1 and IFNGR2 genes may play an important role in the formation of horns, and the P-cadherin gene may be involved in the development of horns.
【作者單位】： 中國農(nóng)業(yè)科學(xué)院蘭州畜牧與獸藥研究所;甘肅省牦牛繁育工程重點(diǎn)實(shí)驗(yàn)室;
【基金】：牦牛遺傳資源與育種(CAAS-ASTIP-2014-LIHPS-01) 科技部科技支撐計(jì)劃(2012BAD13B05)
【分類號】：S823.85
【參考文獻(xiàn)】
相關(guān)期刊論文 前3條
1 趙娟花;梁春年;裴杰;褚敏;吳曉云;張良斌;佘平昌;閻萍;;牦牛C1H21orf62基因的克隆、生物信息學(xué)及表達(dá)分析[J];廣東農(nóng)業(yè)科學(xué);2015年05期
2 吳興龍;王棉娟;胡春超;王鵬博;李相運(yùn);;E-鈣粘蛋白抗體對小鼠早期胚胎發(fā)育的影響[J];畜牧獸醫(yī)學(xué)報(bào);2012年08期
3 任強(qiáng);郁楓;王旭強(qiáng);趙云輝;李靜;;牦牛角的形態(tài)發(fā)生及Shh和β-catenin的時(shí)空表達(dá)[J];西北農(nóng)業(yè)學(xué)報(bào);2009年01期
【共引文獻(xiàn)】
相關(guān)期刊論文 前4條
1 佘平昌;吳曉云;梁春年;y嚸，
本文編號：2143018
本文鏈接：http://sikaile.net/yixuelunwen/dongwuyixue/2143018.html
最近更新
教材專著
