不同植酸酶對(duì)常用飼料原料植酸磷的降解效果及在蛋雞生產(chǎn)中的應(yīng)用研究
發(fā)布時(shí)間：2018-07-24 14:53
【摘要】：本文通過體外試驗(yàn)和動(dòng)物試驗(yàn)前后進(jìn)行了三個(gè)試驗(yàn)研究不同植酸酶對(duì)飼料原料植酸磷的降解及在蛋雞生產(chǎn)中的應(yīng)用效果研究。試驗(yàn)一:在溫度37℃和p H值5.5的條件下,研究了分別源于大腸桿菌、畢赤酵母的兩種植酸酶對(duì)玉米、豆粕、菜籽粕、棉籽粕、麩皮5種常用飼料原料中植酸磷降解效率的影響。在0.0,10.0,20.0,40.0,60.0,120.0,180.0,240.0 min分別測(cè)定了植酸磷的降解率。結(jié)果表明:不同飼料原料中植酸磷含量差異較大(P0.05);不同酶、不同飼料原料對(duì)植酸磷的降解率都有極顯著的影響(P0.01);植酸酶B水解菜籽粕240 min降解率極顯著高于植酸酶A(P0.01),兩種植酸酶水解玉米、豆粕、棉粕和麩皮240 min降解率均差異不顯著(P0.05),但植酸酶B水解玉米、豆粕、棉粕和麩皮240 min降解率均大于植酸酶A。說明了畢赤酵母源的植酸酶降解5種飼料原料中植酸磷的總體效果優(yōu)于大腸桿菌源的植酸酶。試驗(yàn)二:通過動(dòng)物消化試驗(yàn),進(jìn)一步觀察分別源于畢赤酵母、大腸桿菌的兩種植酸酶對(duì)玉米、豆粕、菜籽粕、棉籽粕、花生餅常用飼料原料中營養(yǎng)物質(zhì)的養(yǎng)分表觀消化率情況。選用體質(zhì)健康、體重相近的公雞48只,隨機(jī)分為4組,每組12只雞,共強(qiáng)飼4次,每次強(qiáng)飼后雞群恢復(fù)休整10天。各組試驗(yàn)雞分別交替強(qiáng)飼5種試驗(yàn)原料50g/只,連續(xù)2天收集糞便。結(jié)果表明:①玉米的營養(yǎng)物質(zhì)消化率:植酸酶A對(duì)玉米粗蛋白、粗脂肪、鈣、磷、植酸磷、灰分的利用率分別比對(duì)照組高出51.81%、12.90%、34.59%、44.73%、7.67%、36.32%;植酸酶B對(duì)玉米粗蛋白、粗脂肪、鈣、磷、植酸磷、灰分的消化率分別比對(duì)照組高出42.36%、11.58%、27.80%、48.40%、7.08%、24.51%。植酸酶A對(duì)玉米粗蛋白、粗脂肪、鈣及灰分的消化率高于植酸酶B組,差異顯著(P0.05),分別高出16.38%、1.49%、16.41%、15.65%。植酸酶B對(duì)玉米磷的消化率顯著高于植酸酶A(P0.05)。植酸酶A、B組對(duì)玉米的植酸磷的消化率差異不顯著(P0.05)。②豆粕的營養(yǎng)物質(zhì)消化率:植酸酶A對(duì)豆粕粗蛋白、脂肪、鈣、磷、植酸磷及灰分的利用率分別比對(duì)照組高出9.62%、32.63%、18.09%、31.74%、14.06%、28.21%;植酸酶B對(duì)豆粕粗蛋白、脂肪、鈣、磷、植酸磷及灰分的利用率分別比對(duì)照組高出15.26%、34.25%、12.31%、40.73%、15.39%、19.72%。植酸酶A對(duì)豆粕的鈣、灰分的利用率高于植酸酶B,差異顯著(P0.05),分別高出10.28%、10.58%。植酸酶B對(duì)豆粕粗蛋白、粗脂肪、磷的利用率高于植酸酶A6.24%、2.40%、13.18%,差異顯著(P0.05)。植酸酶A、B組對(duì)豆粕的植酸磷的利用率差異不顯著(P0.05)。③菜籽粕的營養(yǎng)物質(zhì)消化率:植酸酶A對(duì)菜籽粕粗蛋白、脂肪、鈣、磷、植酸磷及灰分的利用率分別比對(duì)照組高出11.37%、28.41%、34.30%、26.58%、8.39%、21.54%;植酸酶B對(duì)菜籽粕粗蛋白、脂肪、鈣、磷、植酸磷及灰分的利用率分別比對(duì)照組組高出4.8%、19.34%、20.70%、22.87%、7.80%、23.55%。植酸酶A對(duì)菜籽粕的蛋白、脂肪、鈣、磷的利用率高于植酸酶B,差異顯著(P0.05),分別高出6.91%、11.24%、17.15%、4.81%。植酸酶A、B對(duì)菜籽粕的植酸磷、灰分的利用率差異不顯著(P0.05)。④棉籽粕的營養(yǎng)物質(zhì)消化率:植酸酶A對(duì)棉籽粕粗蛋白、脂肪、鈣、磷、植酸磷及灰分的利用率分別比對(duì)照組高出1.32%、21.55%、34.00%、12.20%、8.25%、25.12%;植酸酶B對(duì)棉籽粕粗蛋白、脂肪、鈣、磷、植酸磷及灰分的利用率分別比不加酶組高出5.35%、14.19%、26.37%、12.02%、5.35%、21.58%。植酸酶A對(duì)棉粕的脂肪、鈣、植酸磷及灰分的利用率高于植酸酶B,差異顯著(P0.05),分別高出8.57%、10.36%、3.07%、4.51%。植酸酶B對(duì)棉粕粗蛋白的利用率高于植酸酶A組4.08%,差異顯著(P0.05)。植酸酶A、B對(duì)棉粕的磷的利用率差異不顯著(P0.05)。⑤花生餅的營養(yǎng)物質(zhì)消化率:植酸酶A對(duì)花生餅粗蛋白、脂肪、鈣、磷、植酸磷及灰分的利用率分別比對(duì)照組高出7.50%、6.66%、29.07%、16.45%、12.52%、24.01%;植酸酶B對(duì)花生餅粗蛋白、脂肪、鈣、磷、植酸磷及灰分的利用率分別比對(duì)照組高出5.39%、3.54%、14.19%、13.10%、11.77%、14.77%。植酸酶A對(duì)花生餅的粗蛋白、脂肪、鈣、磷及灰分的利用率高于植酸酶B,差異顯著(P0.05),分別高出2.23%、3.23%、17.33%、3.86%、10.84%。植酸酶A、B對(duì)花生餅的植酸磷的利用率差異不顯著(P0.05)。試驗(yàn)三:通過動(dòng)物試驗(yàn),對(duì)比研究了蛋雞日糧添加兩種商品植酸酶對(duì)蛋雞生長性能、血液指標(biāo)以及營養(yǎng)物質(zhì)養(yǎng)分表觀消化率的影響。試驗(yàn)選用體質(zhì)健康、體重相近、生產(chǎn)性能均勻的10周齡京白“939”育成雞200只,隨機(jī)分為5組,每組4個(gè)重復(fù),每個(gè)重復(fù)10只雞。Ⅰ組為對(duì)照組,Ⅱ、Ⅳ組分別添加兩種商品植酸酶植酸酶A、B、600U/kg,Ⅲ、Ⅴ組分別添加兩種商品植酸酶植酸酶A、B1200U/kg。試驗(yàn)期為9周,預(yù)飼期1周,正式期8周。結(jié)果表明:⑴植酸酶對(duì)育成雞生產(chǎn)性能的影響試驗(yàn)期各組之間育成雞初始體重、末重、平均耗料量均差異不顯著(P0.05)。Ⅱ組試驗(yàn)期總增重、平均日增重顯著高于Ⅰ、Ⅲ、Ⅳ、Ⅴ組,差異顯著(P0.05),其他各組間差異不顯著(P0.05)。試驗(yàn)期各組之間育成雞料重比差異不顯著(P0.05),但添加植酸酶的Ⅱ、Ⅲ、Ⅳ、Ⅴ組料重比均低于對(duì)照Ⅰ組。其中,Ⅱ組試驗(yàn)期總增重、平均日增重最高,料重比最低。⑵植酸酶對(duì)育成雞血液指標(biāo)的影響植酸酶對(duì)14w育成雞血液血清堿性磷酸酶活性影響差異不顯著(P0.05)。Ⅲ、Ⅴ組14w血清無機(jī)磷含量大于Ⅰ、Ⅳ組,差異極顯著(P0.01);Ⅱ組血清無機(jī)磷含量大于Ⅰ組,差異極顯著(P0.01),高于Ⅳ組,差異顯著(P0.05)。Ⅱ組14w血清無機(jī)鈣含量大于Ⅰ、Ⅴ組,差異顯著(P0.05)。Ⅱ組14w血清谷丙轉(zhuǎn)氨酶活性高于Ⅰ、Ⅳ、Ⅴ組,差異極顯著(P0.01),大于Ⅲ組,差異顯著(P0.05)。18w育成雞血液血清堿性磷酸酶活性、血清鈣濃度、谷丙轉(zhuǎn)氨酶活性較14w均有較大提高,但是,血清無機(jī)磷濃度較14w則有下降趨勢(shì)。Ⅰ組18w血清堿性磷酸酶活性大于Ⅱ組,差異顯著(P0.05)。Ⅲ組18w血清無機(jī)磷含量均大于Ⅰ、Ⅱ、Ⅳ、Ⅴ組,差異極顯著(P0.01);Ⅴ、Ⅱ組血清無機(jī)磷含量大于Ⅰ、Ⅳ組,差異極顯著(P0.01)。Ⅱ組18w血清鈣含量高于Ⅴ組,差異極顯著(P0.01),高于Ⅰ組,差異顯著(P0.05)。Ⅲ組18w血清谷丙轉(zhuǎn)氨酶活性均大于Ⅰ、Ⅳ、Ⅴ組,差異極顯著(P0.01);Ⅱ組谷丙轉(zhuǎn)氨酶活性大于Ⅰ組,差異極顯著(P0.01),高于Ⅳ組,差異顯著(P0.05);其他各組間差異不顯著(P0.05)。⑶植酸酶對(duì)育成雞養(yǎng)分表觀消化率的影響干物質(zhì)表觀消化率而言,Ⅱ組干物質(zhì)表觀消化率高于Ⅰ、Ⅲ及Ⅴ組,差異極顯著(P0.01),高于Ⅳ組,差異顯著(P0.05)。粗蛋白表觀消化率而言,Ⅱ、Ⅳ、Ⅴ組粗蛋白表觀消化率均高于對(duì)照Ⅰ組,差異顯著(P0.05);Ⅲ組蛋白利用率高于Ⅰ組,差異極顯著(P0.01)。粗脂肪表觀消化率而言,Ⅲ、Ⅳ、Ⅴ組粗脂肪表觀消化率大于Ⅰ組,差異極顯著(P0.01),高于Ⅱ組,差異顯著(P0.05)。鈣表觀消化率而言,Ⅱ、Ⅲ、Ⅴ組鈣表觀消化率大于Ⅰ、Ⅳ組,差異極顯著(P0.01)。磷表觀消化率而言,Ⅲ組磷表觀消化率大于Ⅰ、Ⅳ、Ⅴ組,差異極顯著(P0.01);Ⅱ組磷表觀消化率大于Ⅰ組,差異極顯著(P0.01),大于Ⅳ、Ⅴ組,差異顯著(P0.05);Ⅳ、Ⅴ組磷表觀消化率大于Ⅰ組差異顯著(P0.05)。植酸磷表觀消化率而言,Ⅱ、Ⅲ、Ⅴ組植酸磷表觀消化率大于Ⅰ、Ⅳ組,差異極顯著(P0.01);Ⅳ組植酸磷表觀消化率大于Ⅰ組,差異顯著(P0.05)。灰分表觀消化率而言,Ⅴ組灰分表觀消化率大于Ⅰ、Ⅳ組,差異極顯著(P0.01),高于Ⅱ組,差異顯著(P0.05);Ⅲ組灰分表觀消化率大于Ⅰ、Ⅳ組,差異顯著(P0.05);其他組間差異不顯著(P0.05)。本研究結(jié)果表明,植酸酶在體內(nèi)、體外均能有效地降解植酸磷,能夠有效地促進(jìn)營養(yǎng)物質(zhì)的利用吸收。綜合考慮,大腸桿菌源植酸酶作用效果較好。
[Abstract]:In this paper, three experiments were carried out to study the degradation of phytate phosphorus and the application effect of different phytase on feed raw material before and after in vitro experiment and animal test. Experiment 1: under the condition of temperature 37 and P H 5.5, two kinds of phytase derived from Escherichia coli and Pichia pastoris were studied for corn, soybean meal and rapeseed. The effect of the degradation efficiency of phytate phosphorus in 5 kinds of feed raw materials, including meal, cottonseed meal and bran. The degradation rate of phytate phosphorus was measured in 0.0,10.0,20.0,40.0,60.0120.0180.0240.0 min respectively. The results showed that the phosphorus content of phytic acid in different feed materials was different (P0.05), and the degradation rate of phytate phosphorus by different feed materials was very significant. The degradation rate of 240 min by phytase B hydrolyzed rapeseed meal was significantly higher than that of phytase A (P0.01), the degradation rate of 240 min in soybean meal, cottonseed meal and bran was not significant (P0.05), but the degradation rate of phytase B hydrolysate corn, soybean meal, cottonseed meal and bran peel was higher than that of phytase A. indicating that the phytase A. showed that the plant of Pichia pastoris was planted by phytase. The overall effect of acid enzyme degradation of phytate phosphorus in 5 feedstuff materials is better than that of the phytase of Escherichia coli. Test two: through animal digestion test, the apparent digestibility of nutrients in maize, soybean meal, rapeseed meal, cottonseed meal, and the common feed raw material from two kinds of phytase derived from Pichia pastoris and Escherichia coli were further observed. 48 roosters with physical health and similar weight were randomly divided into 4 groups, 12 chickens in each group, 12 chickens in each group, 4 strong forage and 10 days after each strong feeding. 5 kinds of experimental raw materials were used to collect the feces for 2 days for 2 days. The results showed: (1) the nutrient digestibility of maize: phytase A to corn crude protein and coarse protein The utilization of fat, calcium, phosphorus, phosphorus, phytate and ash were 51.81%, 12.90%, 34.59%, 44.73%, 7.67%, 36.32% higher than that of the control group, and the digestibility of phytase B to corn crude protein, crude fat, calcium, phosphorus, phytate phosphorus and ash was 42.36%, 11.58%, 27.80%, 48.40%, 7.08%, and 24.51%. phytase A to corn crude protein, crude fat, calcium and ash, respectively. The digestibility of the phytase B group was higher than that of the phytase B group (P0.05), which was higher than 16.38%, 1.49%, 16.41% respectively. The digestibility of 15.65%. phytase B was significantly higher than that of phytase A (P0.05). Phytase A, B group had no significant difference in the digestibility of phytate phosphorus in corn (P0.05). (2) the nutrient digestibility of soybean meal: phytase A to the crude protein, fat and calcium of soybean meal The utilization ratio of phosphorus, phytate phosphorus and ash was 9.62%, 32.63%, 18.09%, 31.74%, 14.06%, 28.21% higher than that of the control group, and the utilization rate of phytase B to soybean meal crude protein, fat, calcium, phosphorus, phytate phosphorus and ash were 15.26%, 34.25%, 12.31%, 40.73%, 15.39%, and 19.72%. phytase A was higher than phytase. B, the difference was significant (P0.05), higher than 10.28%. The utilization rate of 10.58%. phytase B on soybean meal crude protein, crude fat and phosphorus was higher than that of phytase A6.24%, 2.40%, 13.18%, and the difference was significant (P0.05). The utilization ratio of phytase A, B group to soybean meal was not significant (P0.05). (3) the nutrient digestibility of rapeseed meal: phytase A to rapeseed meal crude protein, The utilization rate of fat, calcium, phosphorus, phytate phosphorus and ash were 11.37%, 28.41%, 34.30%, 26.58%, 8.39%, 21.54% higher than that of the control group, and the utilization rate of phytase B to rapeseed meal crude protein, fat, calcium, phosphorus, phytate phosphorus and ash were 4.8%, 19.34%, 20.70%, 22.87%, 7.80%, 23.55%. A to rapeseed meal protein, fat, calcium, phosphorus. The utilization rate was higher than that of phytase B, the difference was significant (P0.05), 6.91%, 11.24%, 17.15%, 4.81%. phytase A, B to rapeseed meal phytate phosphorus, the difference of ash utilization ratio was not significant (P0.05). (4) the nutrient digestibility of cottonseed meal: the utilization ratio of Phytase A to cottonseed meal coarse egg white, fat, calcium, phosphorus, phytate phosphorus and ash 1.32%, 21.55%, 34%, 12.20%, 8.25%, 25.12% higher, the utilization ratio of phytase B to cottonseed meal crude protein, fat, calcium, phosphorus, phytate phosphorus and ash was higher than that in the non enzyme group, respectively 5.35%, 14.19%, 26.37%, 12.02%, 5.35%, and the utilization of 21.58%. phytase A was higher than that of phytase B, the difference was significant (P0.05), respectively, The utilization rate of 8.57%, 10.36%, 3.07%, 4.51%. phytase B to cottonseed crude protein was higher than that of the phytase A group (P0.05). The difference of phosphorus utilization ratio between phytase A and B was not significant (P0.05). 5. The nutrient digestibility of the peanut cake: the utilization ratio of phytase A to the peanut cake crude protein, fat, calcium, phosphorus, phytate phosphorus and ash, respectively The control group was 7.50%, 6.66%, 29.07%, 16.45%, 12.52%, 24.01%. The utilization ratio of phytase B to the peanut cake crude protein, fat, calcium, phosphorus, phytate phosphorus and ash were 5.39%, 3.54%, 14.19%, 13.10%, 11.77%, and the utilization of 14.77%. phytase A was higher than that of phytase B (P0. 05), 2.23%, 3.23%, 17.33%, 3.86%, 10.84%. phytase A and B had no significant difference in the utilization of phytate phosphorus (P0.05). Test three: through animal experiments, the effects of two kinds of Phytase on the growth performance, blood indexes and nutrient nutrient apparent digestibility of laying hens were compared. 200 chickens of 10 week old Beijing white "939" bred chicken were randomly divided into 5 groups, including 4 repetitions in each group and 10 chickens in each group. Group I was the control group, and group II and group IV added two kinds of phytase phytase A, B, 600U/kg, III and V respectively added two kinds of phytase phytase A, B1200U/kg. test period, respectively. For 9 weeks, the pre feeding period was 1 weeks and the official period was 8 weeks. The results showed that: (1) the effect of Phytase on the production performance of the bred chicken was not significant (P0.05). The average daily weight gain of group II was significantly higher than that of I, III, IV and V (P0.05), the difference was significant (P0.05), and the difference between the other groups was significant. It was not significant (P0.05). There was no significant difference in weight ratio between each group in the test period (P0.05), but the weight ratio of group II, III, IV, and V was lower than that of the control group I. Among them, the total weight gain of group II was higher, the average daily gain was the highest and the ratio of weight to weight was the lowest. 2. The effect of Phytase on blood indexes of bred chickens by phytase on blood blood of 14W bred chicken There was no significant difference in the activity of alkaline phosphatase activity (P0.05). The content of serum inorganic phosphorus in group 14W was greater than that in group I and group IV (P0.01); the content of serum inorganic phosphorus in group II was greater than that in group I (P0.01), which was higher than group IV (P0.05). The content of serum inorganic calcium in group II of group II was greater than that in group I, group V (P0.05). (P0.05). Group II 14W The activity of serum alanine transaminase was higher than that in group I, group IV and V, the difference was very significant (P0.01), which was greater than group III, and the difference was significant (P0.05) in the blood serum alkaline phosphatase activity of.18w bred chicken, the serum calcium concentration and the activity of alanine aminotransferase were significantly higher than that of 14W. However, the concentration of serum phosphorus free phosphorus was lower than that of 14W. The serum alkaline phosphatase activity of group I was more than that of 14W. The content of serum inorganic phosphorus in group III of group III was greater than that of group I (P0.05). The content of serum inorganic phosphorus in group 18W was greater than that in group I, II, IV, and V (P0.01). The content of serum inorganic phosphorus in group II was greater than that in group I and group IV (P0.01). The serum calcium content of 18W in group II was higher than group V (P0.01), and the difference was significantly higher than group I (P0.05). Group III 18W serum Valley The activity of alanine aminotransferase was greater than that in group I, group IV and V (P0.01), and the activity of glutamic aminotransferase in group II was greater than group I, the difference was very significant (P0.01), and the difference was significant (P0.05); the difference between other groups was not significant (P0.05). (3) the effect of Phytase on the apparent digestibility of the nutrients of the bred chicken, as far as the apparent digestibility of the dry matter was concerned, group II dry matter The apparent digestibility was higher than that in group I, group III and group (P0.01), which was significantly higher than group IV (P0.05). The apparent digestibility of crude protein in group II, IV and V was higher than that of control group I (P0.05), and the utilization rate of protein in group III was higher than that in group I (P0.01). The apparent digestibility of crude fat in group IV was greater than that in group I (P0.01), and the difference was significant (P0.05). The apparent digestibility of Calcium Apparent Digestibility in group II, III and V group was greater than that in group I, group IV, group IV, the difference was very significant (P0.01). The phosphorus apparent digestibility in group III was greater than that in group I, IV, and V (P0.01); group II (Group II); group II The apparent digestibility of phosphorus was greater than that in group I (P0.01), which was greater than that in group IV and group V (P0.05). The apparent digestibility of phosphorus in group IV was greater than that in group I (P0.05). The apparent digestibility of phytic acid phosphorus in group II, III and V group was larger than that of group I, group IV, group IV, the difference was very significant (P0.01), and the apparent digestibility of phytate phosphorus in group IV was greater than I I I The difference was significant (P0.05). The ash apparent digestibility of group V was greater than that in group I, group IV, group IV, the difference was very significant (P0.01), the difference was significant (P0.05) higher than that of group II (P0.05); the ash apparent digestibility of group III was greater than that of group I, group IV, the difference was significant (P0.05). The results of this study showed that phytase was in body, in vitro It can effectively degrade phytic acid phosphorus and effectively promote the absorption and utilization of nutrients.
【學(xué)位授予單位】：河北農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S831.5
本文編號(hào)：2141731
[Abstract]:In this paper, three experiments were carried out to study the degradation of phytate phosphorus and the application effect of different phytase on feed raw material before and after in vitro experiment and animal test. Experiment 1: under the condition of temperature 37 and P H 5.5, two kinds of phytase derived from Escherichia coli and Pichia pastoris were studied for corn, soybean meal and rapeseed. The effect of the degradation efficiency of phytate phosphorus in 5 kinds of feed raw materials, including meal, cottonseed meal and bran. The degradation rate of phytate phosphorus was measured in 0.0,10.0,20.0,40.0,60.0120.0180.0240.0 min respectively. The results showed that the phosphorus content of phytic acid in different feed materials was different (P0.05), and the degradation rate of phytate phosphorus by different feed materials was very significant. The degradation rate of 240 min by phytase B hydrolyzed rapeseed meal was significantly higher than that of phytase A (P0.01), the degradation rate of 240 min in soybean meal, cottonseed meal and bran was not significant (P0.05), but the degradation rate of phytase B hydrolysate corn, soybean meal, cottonseed meal and bran peel was higher than that of phytase A. indicating that the phytase A. showed that the plant of Pichia pastoris was planted by phytase. The overall effect of acid enzyme degradation of phytate phosphorus in 5 feedstuff materials is better than that of the phytase of Escherichia coli. Test two: through animal digestion test, the apparent digestibility of nutrients in maize, soybean meal, rapeseed meal, cottonseed meal, and the common feed raw material from two kinds of phytase derived from Pichia pastoris and Escherichia coli were further observed. 48 roosters with physical health and similar weight were randomly divided into 4 groups, 12 chickens in each group, 12 chickens in each group, 4 strong forage and 10 days after each strong feeding. 5 kinds of experimental raw materials were used to collect the feces for 2 days for 2 days. The results showed: (1) the nutrient digestibility of maize: phytase A to corn crude protein and coarse protein The utilization of fat, calcium, phosphorus, phosphorus, phytate and ash were 51.81%, 12.90%, 34.59%, 44.73%, 7.67%, 36.32% higher than that of the control group, and the digestibility of phytase B to corn crude protein, crude fat, calcium, phosphorus, phytate phosphorus and ash was 42.36%, 11.58%, 27.80%, 48.40%, 7.08%, and 24.51%. phytase A to corn crude protein, crude fat, calcium and ash, respectively. The digestibility of the phytase B group was higher than that of the phytase B group (P0.05), which was higher than 16.38%, 1.49%, 16.41% respectively. The digestibility of 15.65%. phytase B was significantly higher than that of phytase A (P0.05). Phytase A, B group had no significant difference in the digestibility of phytate phosphorus in corn (P0.05). (2) the nutrient digestibility of soybean meal: phytase A to the crude protein, fat and calcium of soybean meal The utilization ratio of phosphorus, phytate phosphorus and ash was 9.62%, 32.63%, 18.09%, 31.74%, 14.06%, 28.21% higher than that of the control group, and the utilization rate of phytase B to soybean meal crude protein, fat, calcium, phosphorus, phytate phosphorus and ash were 15.26%, 34.25%, 12.31%, 40.73%, 15.39%, and 19.72%. phytase A was higher than phytase. B, the difference was significant (P0.05), higher than 10.28%. The utilization rate of 10.58%. phytase B on soybean meal crude protein, crude fat and phosphorus was higher than that of phytase A6.24%, 2.40%, 13.18%, and the difference was significant (P0.05). The utilization ratio of phytase A, B group to soybean meal was not significant (P0.05). (3) the nutrient digestibility of rapeseed meal: phytase A to rapeseed meal crude protein, The utilization rate of fat, calcium, phosphorus, phytate phosphorus and ash were 11.37%, 28.41%, 34.30%, 26.58%, 8.39%, 21.54% higher than that of the control group, and the utilization rate of phytase B to rapeseed meal crude protein, fat, calcium, phosphorus, phytate phosphorus and ash were 4.8%, 19.34%, 20.70%, 22.87%, 7.80%, 23.55%. A to rapeseed meal protein, fat, calcium, phosphorus. The utilization rate was higher than that of phytase B, the difference was significant (P0.05), 6.91%, 11.24%, 17.15%, 4.81%. phytase A, B to rapeseed meal phytate phosphorus, the difference of ash utilization ratio was not significant (P0.05). (4) the nutrient digestibility of cottonseed meal: the utilization ratio of Phytase A to cottonseed meal coarse egg white, fat, calcium, phosphorus, phytate phosphorus and ash 1.32%, 21.55%, 34%, 12.20%, 8.25%, 25.12% higher, the utilization ratio of phytase B to cottonseed meal crude protein, fat, calcium, phosphorus, phytate phosphorus and ash was higher than that in the non enzyme group, respectively 5.35%, 14.19%, 26.37%, 12.02%, 5.35%, and the utilization of 21.58%. phytase A was higher than that of phytase B, the difference was significant (P0.05), respectively, The utilization rate of 8.57%, 10.36%, 3.07%, 4.51%. phytase B to cottonseed crude protein was higher than that of the phytase A group (P0.05). The difference of phosphorus utilization ratio between phytase A and B was not significant (P0.05). 5. The nutrient digestibility of the peanut cake: the utilization ratio of phytase A to the peanut cake crude protein, fat, calcium, phosphorus, phytate phosphorus and ash, respectively The control group was 7.50%, 6.66%, 29.07%, 16.45%, 12.52%, 24.01%. The utilization ratio of phytase B to the peanut cake crude protein, fat, calcium, phosphorus, phytate phosphorus and ash were 5.39%, 3.54%, 14.19%, 13.10%, 11.77%, and the utilization of 14.77%. phytase A was higher than that of phytase B (P0. 05), 2.23%, 3.23%, 17.33%, 3.86%, 10.84%. phytase A and B had no significant difference in the utilization of phytate phosphorus (P0.05). Test three: through animal experiments, the effects of two kinds of Phytase on the growth performance, blood indexes and nutrient nutrient apparent digestibility of laying hens were compared. 200 chickens of 10 week old Beijing white "939" bred chicken were randomly divided into 5 groups, including 4 repetitions in each group and 10 chickens in each group. Group I was the control group, and group II and group IV added two kinds of phytase phytase A, B, 600U/kg, III and V respectively added two kinds of phytase phytase A, B1200U/kg. test period, respectively. For 9 weeks, the pre feeding period was 1 weeks and the official period was 8 weeks. The results showed that: (1) the effect of Phytase on the production performance of the bred chicken was not significant (P0.05). The average daily weight gain of group II was significantly higher than that of I, III, IV and V (P0.05), the difference was significant (P0.05), and the difference between the other groups was significant. It was not significant (P0.05). There was no significant difference in weight ratio between each group in the test period (P0.05), but the weight ratio of group II, III, IV, and V was lower than that of the control group I. Among them, the total weight gain of group II was higher, the average daily gain was the highest and the ratio of weight to weight was the lowest. 2. The effect of Phytase on blood indexes of bred chickens by phytase on blood blood of 14W bred chicken There was no significant difference in the activity of alkaline phosphatase activity (P0.05). The content of serum inorganic phosphorus in group 14W was greater than that in group I and group IV (P0.01); the content of serum inorganic phosphorus in group II was greater than that in group I (P0.01), which was higher than group IV (P0.05). The content of serum inorganic calcium in group II of group II was greater than that in group I, group V (P0.05). (P0.05). Group II 14W The activity of serum alanine transaminase was higher than that in group I, group IV and V, the difference was very significant (P0.01), which was greater than group III, and the difference was significant (P0.05) in the blood serum alkaline phosphatase activity of.18w bred chicken, the serum calcium concentration and the activity of alanine aminotransferase were significantly higher than that of 14W. However, the concentration of serum phosphorus free phosphorus was lower than that of 14W. The serum alkaline phosphatase activity of group I was more than that of 14W. The content of serum inorganic phosphorus in group III of group III was greater than that of group I (P0.05). The content of serum inorganic phosphorus in group 18W was greater than that in group I, II, IV, and V (P0.01). The content of serum inorganic phosphorus in group II was greater than that in group I and group IV (P0.01). The serum calcium content of 18W in group II was higher than group V (P0.01), and the difference was significantly higher than group I (P0.05). Group III 18W serum Valley The activity of alanine aminotransferase was greater than that in group I, group IV and V (P0.01), and the activity of glutamic aminotransferase in group II was greater than group I, the difference was very significant (P0.01), and the difference was significant (P0.05); the difference between other groups was not significant (P0.05). (3) the effect of Phytase on the apparent digestibility of the nutrients of the bred chicken, as far as the apparent digestibility of the dry matter was concerned, group II dry matter The apparent digestibility was higher than that in group I, group III and group (P0.01), which was significantly higher than group IV (P0.05). The apparent digestibility of crude protein in group II, IV and V was higher than that of control group I (P0.05), and the utilization rate of protein in group III was higher than that in group I (P0.01). The apparent digestibility of crude fat in group IV was greater than that in group I (P0.01), and the difference was significant (P0.05). The apparent digestibility of Calcium Apparent Digestibility in group II, III and V group was greater than that in group I, group IV, group IV, the difference was very significant (P0.01). The phosphorus apparent digestibility in group III was greater than that in group I, IV, and V (P0.01); group II (Group II); group II The apparent digestibility of phosphorus was greater than that in group I (P0.01), which was greater than that in group IV and group V (P0.05). The apparent digestibility of phosphorus in group IV was greater than that in group I (P0.05). The apparent digestibility of phytic acid phosphorus in group II, III and V group was larger than that of group I, group IV, group IV, the difference was very significant (P0.01), and the apparent digestibility of phytate phosphorus in group IV was greater than I I I The difference was significant (P0.05). The ash apparent digestibility of group V was greater than that in group I, group IV, group IV, the difference was very significant (P0.01), the difference was significant (P0.05) higher than that of group II (P0.05); the ash apparent digestibility of group III was greater than that of group I, group IV, the difference was significant (P0.05). The results of this study showed that phytase was in body, in vitro It can effectively degrade phytic acid phosphorus and effectively promote the absorption and utilization of nutrients.
【學(xué)位授予單位】：河北農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S831.5
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