【摘要】：布氏桿菌病是由布氏桿菌(Brucella)引起的人獸共患傳染病,以母畜流產(chǎn)、不孕和公畜睪丸炎為特征。新孢子蟲病(Neosporiasis)是多種動物的一種原蟲病,其主要癥狀是孕畜流產(chǎn)、死胎和新生兒運(yùn)動障礙等。牛布氏桿菌病和牛新孢子蟲病均是引起牛繁殖障礙的重要傳染性疾病。隨著我國養(yǎng)牛業(yè)集約化程度的不斷提高,牛布氏桿菌病和牛新孢子蟲病已嚴(yán)重阻礙了我國養(yǎng)牛業(yè)的健康發(fā)展。為了解吉林省部分地區(qū)牛布氏桿菌病和牛新孢子蟲病的流行情況,本研究應(yīng)用虎紅平板凝集試驗(yàn)(RBPT)對吉林省延邊地區(qū)和松原地區(qū)共計(jì)168份流產(chǎn)牛血清樣本進(jìn)行了布氏桿菌病檢測,并對其中不同飼養(yǎng)方式、不同牛品種和不同年齡段的100份牛血清樣本進(jìn)行了比較研究。應(yīng)用酶聯(lián)免疫吸附試驗(yàn)(ELISA)對吉林省梅河、松原、長春、吉林和白山等5個地區(qū)的共計(jì)410份牛血清樣本進(jìn)行了牛新孢子蟲抗體檢測,并對其中不同飼養(yǎng)方式、不同牛品種和不同年齡段的193份牛血清樣本進(jìn)行了比較研究。在布氏桿菌病檢測中,松原地區(qū)的牛血清樣本陽性率為35.90%,延邊地區(qū)牛血清樣本陽性率為58.13%。結(jié)果顯示,吉林省松原地區(qū)是牛布氏桿菌病的流行區(qū)域,延邊地區(qū)中延吉市是牛布氏桿菌病流行最為嚴(yán)重區(qū)域,血清抗體陽性率高達(dá)64.00%。在牛新孢子蟲病檢測中,梅河、松原、長春、吉林和白山地區(qū)牛新孢子蟲陽性率分別為19.67%、23.07%、10.53%、16.67%和35.75%。吉林省梅河、松原、長春、吉林和白山地區(qū)是牛新孢子蟲病的流行區(qū)域,其中白山地區(qū)的長白縣牛新孢子蟲病的抗體陽性率高達(dá)39.65%,而且在不同飼養(yǎng)方式和不同品種間存在顯著性差異。通過流行病學(xué)調(diào)查證實(shí),吉林省是牛布氏桿菌病和牛新孢子蟲病的流行地區(qū)。本研究將為吉林省牛布氏桿菌病和牛新孢子病的防控提供科學(xué)的流行病學(xué)資料。
[Abstract]:Brucellosis is a zoonotic infectious disease caused by Brucella (Brucella), characterized by female abortion, infertility and orchitis. Neosporidiosis (Neosporiasis) is a protozoan disease of many animals. Its main symptoms are abortion, stillbirth and movement disorder of newborn. Bovine brucellosis and bovine neosporidiosis are important infectious diseases causing bovine reproductive disorders. With the development of intensive cattle farming in China, brucellosis and neosporidiosis have seriously hindered the healthy development of cattle farming in China. In order to understand the prevalence of brucellosis and neosporidiosis in some areas of Jilin Province, In this study, a total of 168 abortive bovine serum samples from Yanbian and Songyuan areas of Jilin Province were tested for brucellosis by using tiger red plate agglutination test (RBPT). A comparative study of 100 bovine serum samples from different cattle breeds and ages was carried out. A total of 410 bovine serum samples from five regions of Jilin Province, Meihe, Songyuan, Changchun, Jilin and Baishan were tested for antibodies against bovine neosporidium by enzyme linked immunosorbent assay (ELISA). A comparative study of 193 bovine serum samples from different cattle breeds and ages was carried out. In the detection of brucellosis, the positive rate of bovine serum samples was 35.90 in Songyuan and 58.13 in Yanbian. The results showed that Songyuan area of Jilin Province was the epidemic area of brucellosis in cattle, and Yanji city was the most serious area of brucellosis in Yanbian area. The positive rate of serum antibody was as high as 64.00%. In the detection of bovine neosporidiosis, the positive rates of Neosporidium in Meihe, Songyuan, Changchun, Jilin and Baishan areas were 19.67% and 35.75%, respectively. Meimei River, Songyuan, Changchun, Jilin and Baishan regions of Jilin Province are endemic areas of bovine neosporidiosis. The antibody positive rate of bovine neosporidiosis in Changbai county in Baishan area was 39.65, and there were significant differences among different breeding ways and varieties. The epidemiological investigation confirmed that Jilin Province is the epidemic area of brucellosis and Neosporidiosis. This study will provide scientific epidemiological data for the prevention and control of brucellosis and Neosporidiosis in Jilin Province.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S858.23

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 王季秋;劉鳳岐;張萌;;布氏菌病血清學(xué)研究進(jìn)展[J];中國地方病防治雜志;2006年01期

2 郭艷秋;關(guān)玉華;范毓欣;;布氏桿菌病誤診為淋巴瘤1例分析[J];吉林醫(yī)學(xué);2008年07期

3 王建曄;王新軍;;522例布魯氏菌病流行病學(xué)分析[J];疾病監(jiān)測與控制;2009年04期

4 馬利青;;柴達(dá)木地區(qū)黃牛新孢子蟲病的ELISA檢測[J];畜牧與獸醫(yī);2006年04期

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本文編號：2140980