【摘要】：本研究旨在研究接種益生菌發(fā)酵果蔬液體飼料的最佳工藝組合,在最佳發(fā)酵工藝條件下發(fā)酵果蔬液體飼料后用于斷奶仔豬飼養(yǎng)對(duì)比。研究果蔬發(fā)酵液體飼料相對(duì)于顆粒料、常規(guī)液體發(fā)酵飼料及添加蘋果渣的液體發(fā)酵飼料,對(duì)仔豬生長(zhǎng)性能、血液常規(guī)、腸道菌群等指標(biāo)的影響。具體研究結(jié)果如下:試驗(yàn)一:單菌、混菌發(fā)酵果蔬液體飼料最佳工藝的研究本試驗(yàn)通過對(duì)果蔬液體飼料接種酵母菌、枯草芽孢桿菌、植物乳桿菌單菌發(fā)酵的單因素試驗(yàn)和正交試驗(yàn),探討發(fā)酵時(shí)間、發(fā)酵溫度、菌種接種量、飼料pH值等參數(shù)對(duì)果蔬液體飼料營(yíng)養(yǎng)價(jià)值的影響,其研究結(jié)果如下。酵母菌最佳優(yōu)化條件為好氧條件下,發(fā)酵時(shí)間60h、溫度30℃、pH 6.0、接種量3%。在最佳優(yōu)化條件下經(jīng)酵母菌發(fā)酵果蔬液體飼料,粗蛋白含量上升至19.41%,粗纖維含量減小至2.09%,乳酸含量增加約7.38倍,鈣、磷含量也有不同程度的增加。枯草芽孢桿菌最佳優(yōu)化條件為好氧條件下,溫度30℃、發(fā)酵時(shí)間60h、pH 5.0、接種量5%。在最佳優(yōu)化條件下經(jīng)枯草芽孢桿菌發(fā)酵后,粗蛋白含量上升至19.71%,粗纖維含量減小至2.31%,乳酸含量達(dá)到16.98g/L,鈣、磷含量同樣也有不同程度的增加。植物乳桿菌最佳優(yōu)化條件為厭氧條件下,發(fā)酵時(shí)間60h、溫度37℃、接種量3%、pH 6.0。在最佳優(yōu)化條件下經(jīng)植物乳桿菌發(fā)酵后,粗蛋白含量上升至19.38%,粗纖維含量減小至2.37%,乳酸含量達(dá)到62.11 g/L,鈣,磷含量上升不明顯。將上述三種菌按最佳發(fā)酵條件混菌異步發(fā)酵后,飼料營(yíng)養(yǎng)價(jià)值比單一菌種發(fā)酵的營(yíng)養(yǎng)價(jià)值有所提高,干物質(zhì)含量中粗蛋白含量比發(fā)酵前上升9.2%,達(dá)到19.88%;粗纖維含量降低至2.01%;乳酸、生物量、Ca、P也有一定的提升,分別提高至61.88g/L、162.73×106 cfu/ml、0.99%和0.91%。試驗(yàn)二:果蔬發(fā)酵液體飼料對(duì)斷奶仔豬生長(zhǎng)性能、血液常規(guī)、腸道菌群等指標(biāo)的影響隨機(jī)挑選同時(shí)28日齡斷奶的健康仔豬200頭,采用隨機(jī)分組方式分成4個(gè)處理組,分別為A組飼喂顆粒料基礎(chǔ)日糧、B組飼喂液體飼料、C組飼喂含30g/kg蘋果渣的液體飼料、D組飼喂含30g/kg的果蔬混菌發(fā)酵液體飼料。每個(gè)組5個(gè)重復(fù),每個(gè)重復(fù)10頭豬。試驗(yàn)預(yù)試期7天,正試期為30天。飼養(yǎng)試驗(yàn)結(jié)束后,從每個(gè)重復(fù)中選取體形、體重適中的仔豬各一只,收集小腸各段內(nèi)容物用于腸道微生物菌群的測(cè)定。試驗(yàn)結(jié)果表明,在平均日增重方面,A、B、C、D組之間兩兩差異顯著(P0.05),其中D組即果蔬混菌發(fā)酵液體飼料組比A組提高19.01%,比B組提高13.91%,比C組提高10.59%且均差異顯著(P0.05)。在料重比方面,B、C兩組比A組分別降低1.71%和0.57%均差異顯著(P0.05);D組比A組降低10.29%(P0.05,差異顯著)。混菌發(fā)酵果蔬液體飼料可有顯著提高胃腸中乳酸菌含量,顯著降低大腸桿菌含量,對(duì)斷奶仔豬胃腸內(nèi)容物pH值無顯著影響。果蔬混菌發(fā)酵液體飼料能夠提高斷奶仔豬血液生化指標(biāo),降低超氧化物歧化酶和血清尿素氮含量,對(duì)機(jī)體免疫功能沒有負(fù)面影響。
[Abstract]:The purpose of this study was to study the optimum technology combination of fermentation liquid feed of fruits and vegetables with probiotics, and to ferment the liquid feed of fruit and vegetable under the best fermentation conditions, and then use it for weaning piglets. The effects of fermented liquid feed on growth performance, blood routine and intestinal microflora of piglets were studied. The results are as follows: experiment 1: the optimum technology of fermentation of liquid feed for fruit and vegetable by single bacteria and mixed bacteria; this experiment inoculated the liquid feed of fruit and vegetable with yeasts, Bacillus subtilis, Bacillus subtilis and Bacillus subtilis. The effects of fermentation time, fermentation temperature, inoculation amount and feed pH on nutritional value of liquid feed for fruits and vegetables were studied by single factor test and orthogonal experiment of Lactobacillus plantarum fermentation. The results are as follows. The optimum conditions for yeast fermentation were aerobic, fermentation time 60 h, temperature 30 鈩，

本文編號(hào)：2136769