【摘要】：本文旨在研究斷奶仔豬血清氨基酸含量和生化參數(shù)對不同蛋白質(zhì)水平飼糧的動態(tài)響應(yīng)。選取18頭35日齡平均體重為(10.0±1.0)kg三元(杜×長×大)雜交斷奶閹公豬,隨機分為3組(每組6個重復(fù),每個重復(fù)1頭豬),并分別飼喂蛋白質(zhì)水平為14%(LP)、17%(M P)和20%(HP)的飼糧。每頭試驗豬安裝頸動脈血管插管,術(shù)后恢復(fù)5 d,于第6~8天正式試驗。每天采食前,采食后30、60、120、180和300 min連續(xù)采集頸動脈血液10 m L,分別檢測血清游離氨基酸含量和相關(guān)生化指標。結(jié)果表明:不同蛋白質(zhì)水平飼糧對仔豬采食前后各時間點血清中游離賴氨酸(Lys)、蛋氨酸(Met)、蘇氨酸(Thr)、色氨酸(Trp)、天冬氨酸(Asp)、絲氨酸(Ser)、甘氨酸(Gly)和谷氨酸(Glu)含量影響均不顯著(P0.05)。但血清中Lys、Met、Thr、Trp含量隨飼糧蛋白質(zhì)水平的降低而升高。HP組仔豬采食前后各時間點血清游離氨基酸[纈氨酸(Val)、異亮氨酸(Ile)、精氨酸(Arg)]和尿素含量顯著高于MP和LP組(P0.05)。采食后120~300 min HP組血清Leu含量顯著高于LP組(P0.05)。采食后各時間點LP組血清總蛋白(TP)、白蛋白(ALB)含量及堿性磷酸酶(ALP)活性顯著高于HP和MP組(P0.05)。各組仔豬采食后血清游離氨基酸含量和相關(guān)生化指標隨時間變化無顯著差異(P0.05)。綜上所述,斷奶仔豬飼糧在平衡4種氨基酸(Lys、Met、Thr、Trp)的基礎(chǔ)上,常規(guī)飼糧蛋白質(zhì)水平降低3個百分點,血清游離Val、Ile、Arg含量顯著降低,但對血清中其他氨基酸和相關(guān)生化指標無顯著影響;而蛋白質(zhì)水平降低6個百分點,血清游離Leu和尿素含量顯著降低,TP、ALB含量和ALP活性顯著升高,但對血清中其他氨基酸和相關(guān)生化指標的影響不顯著。
[Abstract]:The aim of this study was to study the dynamic response of serum amino acid content and biochemical parameters to diets at different protein levels in weaned piglets. Eighteen 35 day-old weanling boars with an average body weight of (10.0 鹵1.0) kg (du 脳 long 脳 large) were randomly divided into 3 groups (6 repeats in each group, 1 pig per replica) and fed diets with protein level of 14% (LP) 17% (M P) and 20% (HP) respectively. Carotid artery catheterization was installed in each pig and recovered 5 days after operation. 10 mL of carotid blood was collected continuously before and after feeding for 30 min and 300 min. Serum free amino acid content and related biochemical indexes were detected respectively. The results showed that the levels of free lysine (Lys), methionine (Met), threonine (THR), tryptophan (TRP), aspartate (Asp), serine (Ser), glycine (gly) and glutamate (Glu) in serum of piglets fed with different protein levels were not significantly affected (P0.05). But the contents of serum free amino acids [Val, Ile, arginine (Arg)] and urea in HP group were significantly higher than those in MP and LP groups (P0.05). The level of serum Leu in 120,300 min HP group was significantly higher than that in LP group (P 0.05). The levels of serum total protein (TP), albumin (ALB) and alkaline phosphatase (ALP) activity in LP group were significantly higher than those in HP and MP groups (P0.05). There was no significant difference in serum free amino acid content and related biochemical indexes with time after feeding in each group (P0.05). In conclusion, on the basis of the balance of four amino acids in weaned piglets diet, the protein level of conventional diet decreased by 3 percentage points, and the content of free Valle Iletio Arg in serum decreased significantly, but there was no significant effect on other amino acids and related biochemical indexes in serum. However, the content of free Leu and urea in serum decreased significantly, and the content of ALB and the activity of ALP increased significantly, but the effect on other amino acids and related biochemical indexes was not significant.
【作者單位】： 中國科學(xué)院亞熱帶農(nóng)業(yè)生態(tài)所亞熱帶農(nóng)業(yè)生態(tài)過程重點實驗室湖南省畜禽健康養(yǎng)殖工程技術(shù)研究中心農(nóng)業(yè)部中南動物營養(yǎng)與飼料科學(xué)觀測試驗站;中國科學(xué)院大學(xué);湘潭大學(xué);廣東省旺大集團豬清潔飼料技術(shù)研發(fā)院士工作站;湖南畜禽安全生產(chǎn)協(xié)同創(chuàng)新中心;
【基金】：國家973課題(2013CB127301) 國家科技支撐計劃課題(2013BAD21B04)
【分類號】：S828.5

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