發(fā)布時(shí)間：2018-07-05 19:33

  本文選題：酵母壁多糖 + 斷奶仔豬��； 參考：《中國(guó)畜牧雜志》2017年09期

【摘要】：本試驗(yàn)旨在探討飼糧中添加酵母壁多糖對(duì)斷奶仔豬盲腸菌群結(jié)構(gòu)的影響。采用單因素試驗(yàn)設(shè)計(jì),選取180頭遺傳背景相同、胎次相近、體重接近的21日齡斷奶仔豬,隨機(jī)分為4個(gè)處理,每個(gè)處理5個(gè)重復(fù),每個(gè)重復(fù)9頭豬。4個(gè)處理分別飼喂0%酵母壁多糖飼糧(對(duì)照組)、0.15%酵母壁多糖飼糧(0.15%組)、0.30%酵母壁多糖飼糧(0.30%組)和0.45%酵母壁多糖飼糧(0.45%組)。試驗(yàn)期為21 d。取盲腸內(nèi)容物提取細(xì)菌DNA,PCR擴(kuò)增獲得16S r RNA基因V4標(biāo)簽片段,進(jìn)行Mi Seq高通量測(cè)序。結(jié)果表明:當(dāng)酵母壁多糖添加量達(dá)一定程度時(shí),仔豬盲腸菌群多樣性會(huì)發(fā)生改變;在不考慮群落中每個(gè)物種豐度的前提下,0.30%酵母壁多糖組仔豬盲腸菌群最豐富;仔豬盲腸優(yōu)勢(shì)菌群為擬桿菌門、厚壁菌門、螺旋體門和變形菌門;與對(duì)照組相比,添加0.30%酵母壁多糖能降低仔豬腸道擬桿菌門含量(P0.05),添加0.30%或0.45%酵母壁多糖能顯著增加盲腸厚壁菌門含量(P0.05),添加0.45%酵母壁多糖還能提高仔豬盲腸軟壁菌門含量(P0.05);酵母壁多糖具有降低盲腸變形菌門含量的趨勢(shì)(P=0.066);普氏菌屬為仔豬盲腸優(yōu)勢(shì)菌屬,酵母壁多糖能顯著提高仔豬盲腸厭氧弧菌屬、瘤胃桿菌屬、糞球菌屬和考拉桿菌屬的含量(P0.05),顯著降低鏈球菌屬的含量(P0.05)。由此可知,當(dāng)酵母壁多糖添加量達(dá)到一定水平時(shí),短期添加可提高斷奶仔豬盲腸厚壁菌門以及瘤胃桿菌屬和糞球菌屬等優(yōu)勢(shì)菌群的含量。
[Abstract]:The purpose of this study was to investigate the effect of yeast polysaccharide on the structure of cecal flora in weaned piglets. 180 weaned piglets with the same genetic background, similar birth order and close weight were randomly divided into 4 treatments with 5 replicates each. Four treatments were fed 0% yeast wall polysaccharide diet (control group) 0.15% yeast wall polysaccharide diet (0.15% group) 0.30% yeast wall polysaccharide diet (0.30% group) and 0.45% yeast wall polysaccharide diet (0.45% group) respectively. The experimental period is 21 days. The 16s rRNA gene V4 tag fragment was amplified by PCR from the cecum contents of bacteria DNA. The Mi Seq high throughput sequencing was carried out. The results showed that the diversity of cecum microflora of piglets would change when the amount of polysaccharide added to yeast wall reached a certain level, and the cecum flora of piglets with 0.30% polysaccharide from yeast wall was the most abundant without considering the abundance of each species in the community. The dominant bacteria in cecum of piglets were Bacteroides phylum, Mycoderma thuringiensis, Spirulina phylum and Proteus phylum. Adding 0.30% polysaccharide to yeast wall could decrease the content of Bacteroides sp. (P0.05), adding 0.30% or 0.45% polysaccharide of yeast wall could significantly increase the content of Cecum phylum (P0.05), and adding 0.45% polysaccharide of yeast wall could also increase the content of phylum caecum of piglets (P0.05). The polysaccharide of mother wall has the tendency of decreasing the content of Proteus cecum (P0. 066), the genus Pueraria belongs to the dominant genus of cecum in piglets. Yeast wall polysaccharides could significantly increase the contents of the genus Vibrio cecum, the genus Rumen, the genus faecococcus and the genus koala (P0.05), and significantly reduce the content of Streptococcus (P0.05). It can be seen that when the amount of polysaccharide in yeast wall reaches a certain level, the short-term addition can increase the content of the dominant bacteria such as Cecum of weaning piglets, Rumen bacillus and faecococcus.
【作者單位】： 江西農(nóng)業(yè)大學(xué)動(dòng)物科技學(xué)院江西省動(dòng)物營(yíng)養(yǎng)重點(diǎn)實(shí)驗(yàn)室江西省營(yíng)養(yǎng)飼料開發(fā)工程中心;江西中醫(yī)藥大學(xué)實(shí)驗(yàn)動(dòng)物科技中心;
【基金】：江西省重大科技專項(xiàng)(20143ACF60001) 江西省生豬產(chǎn)業(yè)技術(shù)體系(JXARS-03-營(yíng)養(yǎng)與飼料崗)
【分類號(hào)】：S828.5

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