動(dòng)物益生菌短短芽胞桿菌FJAT-1501-BPA制劑的制備及其益生特性評(píng)價(jià)
發(fā)布時(shí)間:2018-07-01 19:54
本文選題:短短芽胞桿菌 + 人工胃液 ; 參考:《中國(guó)畜牧獸醫(yī)》2017年10期
【摘要】:為優(yōu)化短短芽胞桿菌FJAT-1501-BPA膠懸劑配方,獲得性狀穩(wěn)定可長(zhǎng)期貯存的菌劑,同時(shí)對(duì)其作為益生菌進(jìn)行益生特性評(píng)價(jià),本試驗(yàn)研究了不同穩(wěn)定劑、p H、Na Cl及防腐劑濃度對(duì)短短芽胞桿菌FJAT-1501-BPA發(fā)酵液保存特性的影響,并對(duì)其產(chǎn)芽孢率、耐熱性、抗生素敏感性、人工胃腸液耐受性、膽鹽耐受性等生物學(xué)特性進(jìn)行評(píng)估。結(jié)果顯示,添加4%Na Cl、0.1‰檸檬酸鈣、0.1‰雙乙酸鈉、0.1‰山梨酸鉀、2.0‰黃原膠,調(diào)節(jié)p H為7,可作為短短芽胞桿菌FJAT-1501-BPA膠懸劑保存的參考條件。短短芽胞桿菌FJAT-1501-BPA能夠抑制沙門(mén)氏菌、大腸桿菌、金黃色葡萄球菌3種動(dòng)物腸道病原菌的生長(zhǎng),具有產(chǎn)孢率高、耐熱、耐酸、耐膽鹽的特點(diǎn),可與鏈霉素、氨芐西林同時(shí)使用,益生效果良好。因此,短短芽胞桿菌FJAT-1501-BPA可作為益生菌菌株應(yīng)用于畜牧生產(chǎn)。
[Abstract]:In order to optimize the formula of Bacillus short strain FJAT-1501-BPA gel suspension, a stable and long-term storage agent was obtained, and the probiotics were evaluated as probiotics. In this study, the effects of different stabilizers and preservative concentrations on the preservation characteristics of Bacillus brevis FJAT-1501-BPA fermentation broth were studied, and the spore-producing rate, heat resistance, antibiotic sensitivity and tolerance of artificial gastrointestinal fluid were also studied, and the effects of different stabilizers and preservatives on the preservation characteristics of the fermentation broth of Bacillus brevis FJAT-1501-BPA were studied. Bile salt tolerance and other biological characteristics were evaluated. The results showed that the addition of NaCl-0.1 鈥,
本文編號(hào):2088974
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