本文選題：雞球蟲病 + 弱毒疫苗�。� 參考：《西北農(nóng)林科技大學(xué)》2015年碩士論文

【摘要】：球蟲病一直是集約化養(yǎng)雞場危害比較大的雞病之一,發(fā)病率高達50%~70%,死亡率20%~30%,嚴重者高達80%。每年,全世界因為球蟲病造成的經(jīng)濟損失超過30億美元。隨著抗球蟲藥物的發(fā)現(xiàn)和使用,球蟲病已不再是毀滅性的,但是隨著耐藥蟲株的產(chǎn)生,以及藥物殘留、食品安全、新藥研究缺乏等問題的出現(xiàn),大家開始將目標轉(zhuǎn)移到用疫苗預(yù)防球蟲病,疫苗將會是不久后防制球蟲病的主要措施。為了評價西安某公司所生產(chǎn)的雞柔嫩艾美耳球蟲、巨型艾美耳球蟲、毒害艾美耳球蟲三價弱毒疫苗的安全性和免疫效果,開展了本項試驗。(1)安全性試驗:給3日齡雛雞接種10倍、100倍超劑量的疫苗,通過觀察臨床癥狀、死亡率、血便情況、剖檢腸道病變等幾個方面,對疫苗的安全性進行合理的評價。結(jié)果顯示,對照組、10倍免疫組、100倍免疫組均無死亡,血便情況和剖檢腸道病變均符合標準,疫苗的安全性合格。(2)效力試驗:給免疫組雛雞在3日齡、8日齡、16日齡分別進行一免、二免、三免,7天后強毒卵囊分別攻擊免疫組和對照組。通過觀察臨床癥狀、死亡率、血便情況、剖檢腸道病變等幾個方面,對疫苗的免疫效果進行合理的評價。結(jié)果顯示,對照組雛雞死亡率為30%,免疫組雛雞死亡率為0%,且免疫后的雛雞從臨床癥狀、血便情況、剖檢腸道病變等多方面均優(yōu)于對照組。表明,疫苗免疫效果良好。
[Abstract]:Coccidiosis has always been one of the most harmful chicken diseases in intensive chicken farms. The incidence of coccidiosis is as high as 50% and 70%, the mortality rate is 20% and 30%, and the serious one is as high as 80%. Worldwide, economic losses from coccidiosis exceed $3 billion a year. With the discovery and use of anti-coccidiosis drugs, coccidiosis is no longer destructive, but with the emergence of drug-resistant strains, as well as drug residues, food safety, lack of new drug research and other problems, People are beginning to switch to vaccines to prevent coccidiosis, which will soon be the main measure of coccidiosis control. In order to evaluate the safety and immune effect of the three valent attenuated vaccine produced by a company in Xi'an, Eimeria tenella and Eimeria mega were poisoned. The experiment was carried out. (1) Safety test: 10 times 100 times overdose vaccine was given to 3-day-old chicks. The clinical symptoms, mortality, blood stool and intestinal lesions were observed. The safety of the vaccine was evaluated reasonably. The results showed that there was no death, blood stool and pathological changes of intestinal tract were in accordance with the standard, and the vaccine was safe. (2) efficacy test: the chickens in the immunized group were immunized at the age of 3 days, 8 days old and 16 days old, respectively. After 7 days, the virulent oocysts attacked the immunized group and the control group respectively. The immune effect of the vaccine was evaluated by observing clinical symptoms, mortality, blood stool and intestinal lesions. The results showed that the mortality rate of the control group was 30 and that of the immunized chicks was 0. The immunized chicks were superior to the control group in many aspects such as clinical symptoms, blood stool, intestinal lesions and so on. The results showed that the immune effect of the vaccine was good.
【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S858.31

【共引文獻】

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