發(fā)布時(shí)間：2018-06-26 09:25

  本文選題：豬 + 精液保存　； 參考：《廣東海洋大學(xué)》2017年碩士論文

【摘要】：豬人工授精(Artificial Insemination;AI)技術(shù)在生產(chǎn)上已廣泛應(yīng)用,精液作為人工授精技術(shù)的關(guān)鍵環(huán)節(jié),其保存技術(shù)越來(lái)越受到重視,但還存在諸多問(wèn)題。低溫保存簡(jiǎn)單經(jīng)濟(jì),但技術(shù)仍然不成熟,目前還處于稀釋液篩選階段,冷凍保存雖然能逾越各種界限,但在全球的使用率卻僅僅只有1%。而且精液的保存技術(shù)卻時(shí)刻制約著人工授精的效果,為了進(jìn)一步提高人工授精的利用率和精液保存技術(shù)的推廣,本文對(duì)上述問(wèn)題進(jìn)行了3項(xiàng)研究,試驗(yàn)1是以長(zhǎng)白公豬精液為試驗(yàn)對(duì)象,以雌二醇、睪酮、黃體酮、ATP、BSA為添加劑,分別以不同濃度加入Tris卵黃稀釋液、Modena稀釋液、Modena+sk稀釋液中低溫保存,進(jìn)行最佳添加劑、最有效添加濃度、以及最佳稀釋液的篩選;試驗(yàn)2以長(zhǎng)白公豬精液為試驗(yàn)對(duì)象,添加不同濃度的甘油、卵黃、以及不同糖類到Tris卵黃稀釋液中冷凍保存,探索甘油、卵黃的最佳添加濃度,以及不同糖類的改善效果;試驗(yàn)3以長(zhǎng)白、大白、杜洛克母豬為實(shí)驗(yàn)對(duì)象,分別使用鮮精與冷凍精液進(jìn)行人工授精對(duì)比試驗(yàn),探討冷凍精液在規(guī)�；a(chǎn)中的使用效果,結(jié)果如下:試驗(yàn)1:最佳濃度與最佳添加劑:在Tris卵黃稀釋液中,10μg/ml雌二醇、20μg/ml睪酮、80μg/ml黃體酮、0.025mg/ml ATP、0.05、0.1mg/ml BSA是改善低溫保存效果的最有效濃度,其中保存效果最好的是80μg/ml黃體酮。最佳稀釋液:改善效果最好的稀釋液是Modena+sk(脫脂乳)稀釋液,其次是Tris卵黃稀釋液,最后是Modena稀釋液,其中以Modena+sk+0.05mg/mlBSA(P0.05)的組合對(duì)豬精液5℃保存的改善效果最好。試驗(yàn)2:長(zhǎng)白豬精液在本實(shí)驗(yàn)中,3%濃度的甘油對(duì)精液品質(zhì)具的改善效果較明顯(P0.05),過(guò)多的甘油體現(xiàn)出其毒性作用,對(duì)精液品質(zhì)無(wú)改善甚至全部死亡。20%含量的卵黃對(duì)精液品質(zhì)的改善效果較明顯(P0.05),過(guò)多或過(guò)少的濃度均呈現(xiàn)出一定的副作用,對(duì)精液品質(zhì)無(wú)改善甚至全部死亡。海藻糖與果糖對(duì)精液品質(zhì)的改善效果比其他糖類明顯(P0.05),其次是蔗糖與乳糖的,最差的是葡萄糖。試驗(yàn)3:凍精活力34±3%,鮮精93±2%,凍精解凍后的精液品質(zhì)均明顯低于鮮精組(P0.05)。凍精組母豬的受胎率(50-66%)、分娩率(73-90%)、窩均產(chǎn)仔數(shù)(6.3-8.7)、窩均產(chǎn)活仔數(shù)(5.3-7.5)均低于鮮精組(P0.05)。精子活力低是導(dǎo)致其繁殖成績(jī)差,以及不能應(yīng)用在規(guī)�；i場(chǎng)上的主要原因之一。從試驗(yàn)觀察上總結(jié):凍精首次輸精時(shí)間延遲3-6小時(shí)是可行的。杜洛克母豬繁殖效果最差。有輕微生殖道疾病的母豬,使用凍精難以使其懷孕,換成鮮精卻可馬上受孕。涼爽天氣受胎效果比較理想。本研究展示了豬精液低溫保存、冷凍保存中各種添加劑,以及冷凍精液人工授精的使用效果,未來(lái)還要進(jìn)一步探索其他添加劑以及組合對(duì)精液保存的作用,以及在冷凍精液人工授精的操作規(guī)程上作出更深入的制定。
[Abstract]:Pig artificial insemination (AI) technology has been widely used in production. Semen, as a key link of artificial insemination technology, has been paid more and more attention to, but there are still many problems. Cryopreservation is simple and economical, but the technology is still immature. At present, it is still in the stage of dilution screening. Although cryopreservation can cross all kinds of limits, the utilization rate of cryopreservation is only 1% in the world. In order to further improve the utilization rate of artificial insemination and the popularization of semen preservation technology, this paper has carried out three studies on the above problems, in order to further improve the utilization rate of artificial insemination and the popularization of semen preservation technology. In experiment 1, the semen of Landrace boar was used as experiment object, estradiol, testosterone and progesterone ATP BSA were used as additives, and different concentrations of Tris egg yolk diluent Modena dilution were added to Modena diluent at low temperature to preserve the best additive. The most effective concentration and the best diluent were screened. In experiment 2, different concentrations of glycerol, yolk, and different sugars were added to Tris yolk diluent to explore glycerol. The optimum concentration of egg yolk and the improvement effect of different sugars. Experiment 3: contrast experiment of artificial insemination with fresh semen and frozen semen with Landrace, white and Duroc sows, The results were as follows: experiment 1: the best concentration and additive: 10 渭 g/ml estradiol 20 渭 g/ml testosterone 80 渭 g/ml progesterone 0.025 mg / ml ATP 0.05 mg / ml BSA is the most effective concentration to improve the cryopreservation effect. The best preservation effect was 80 渭 g/ml progesterone. The best diluent: Modena sk (skim milk), Tris yolk dilution and Modena diluent were the best. Modena SK 0.05 mg / ml BSA (P0.05) had the best effect on the preservation of porcine semen at 5 鈩，

本文編號(hào)：2069914