本文選題：微生態(tài)制劑 + 側(cè)孢短芽孢桿菌S-��； 參考：《中國食品學(xué)報》2017年01期

【摘要】：通過研究側(cè)孢短芽孢桿菌S62-9的抑菌譜及抑菌活性,初步判斷其是否符合微生態(tài)制劑菌種選育的標(biāo)準(zhǔn)。通過指示平板法研究側(cè)孢短芽孢桿菌S62-9的抑菌譜,從中選取1株藤黃微球菌28001(G~+)和1株大腸桿菌CMCC 44752(G~-)作為指示菌,采用液態(tài)共培養(yǎng)法研究S62-9對兩株菌的抑菌能力。結(jié)果表明,S62-9對大多數(shù)致病性細(xì)菌及部分真菌產(chǎn)生抑菌作用,對G~+菌的抑菌效果強于G~-菌;液態(tài)共培養(yǎng)體系中,S62-9對兩株指示菌表現(xiàn)出強烈的抑菌作用,抑菌效果隨其所占接種比例的增加而增強,抑菌機(jī)理包括營養(yǎng)競爭作用以及S62-9產(chǎn)生的抗菌肽的拮抗作用。該研究表明,側(cè)孢短芽孢桿菌S62-9具有廣譜、高效的抑菌特性,為其微生態(tài)制劑的開發(fā)提供了理論基礎(chǔ)。
[Abstract]:By studying the bacteriostatic spectrum and antibacterial activity of Bacillus cereus S62-9, it was preliminarily determined whether it met the criteria of microecological preparation strain selection. The bacteriostatic spectrum of Bacillus cereus S62-9 was studied by means of indicating plate method. One strain of Micrococcus luteum 28001 (G~) and one strain of Escherichia coli CMCC 44752 (GC-) were selected as indicator bacteria. The bacteriostatic ability of S62-9 against two strains was studied by liquid co-culture method. The results showed that S62-9 had bacteriostatic effect on most pathogenic bacteria and some fungi, and had stronger bacteriostatic effect on G ~ bacteria than on Gauricobacterium, and that S62-9 had strong bacteriostatic effect on two indicating bacteria in liquid co-culture system. The bacteriostatic effect increased with the increase of inoculation proportion. The bacteriostatic mechanism included nutrition competition and antagonism of antimicrobial peptides produced by S62-9. The results showed that S62-9 had broad spectrum and high efficiency bacteriostasis, which provided a theoretical basis for the development of microecological agents.
【作者單位】： 河北農(nóng)業(yè)大學(xué)食品科技學(xué)院;河北科技大學(xué)生物科學(xué)與工程學(xué)院;北京工商大學(xué)食品學(xué)院;
【基金】：“十二五”國家科技支撐計劃項目(2013BAD10B03) 國家農(nóng)業(yè)科技成果轉(zhuǎn)化項目(2013GB2A200032) 河北省自然科學(xué)基金項目(C2014208137)
【分類號】：S816

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本文編號：2061310