本文選題：脫氧雪腐鐮刀菌烯醇 + 海馬神經(jīng)細(xì)胞�。� 參考：《中國獸醫(yī)科學(xué)》2017年01期

【摘要】：為了研究脫氧雪腐鐮刀菌烯醇(deoxynivalenol,DON)對仔豬海馬神經(jīng)細(xì)胞的毒性作用,于體外培養(yǎng)仔豬海馬神經(jīng)細(xì)胞至對數(shù)生長期,以0、125、250、500、1 000和2 000 ng/m L不同質(zhì)量濃度的DON對細(xì)胞進(jìn)行染毒處理,應(yīng)用倒置顯微鏡和透射電鏡觀察細(xì)胞的形態(tài)學(xué)變化;Hoechest 33258染色液對細(xì)胞核進(jìn)行標(biāo)記,觀察細(xì)胞核的形態(tài)學(xué)變化;CCK-8試劑盒檢測細(xì)胞的存活率、乳酸脫氫酶(LDH)試劑盒檢測LDH漏出率。結(jié)果顯示,與對照組相比,處理組細(xì)胞發(fā)生了明顯的形態(tài)學(xué)變化,隨著DON質(zhì)量濃度增加,細(xì)胞密度降低、空泡化、染色質(zhì)濃縮聚集、產(chǎn)生凋亡小體;處理組細(xì)胞的存活率明顯降低,具有劑量和時間依賴性。LDH漏出率隨著DON質(zhì)量濃度的增加而增加,與對照組相比,當(dāng)質(zhì)量濃度達(dá)到500 ng/m L以上時,差異極顯著(P0.01)。試驗表明,DON對仔豬海馬神經(jīng)細(xì)胞具有明顯的毒性作用,能夠誘導(dǎo)細(xì)胞損傷,發(fā)生凋亡。
[Abstract]:In order to study the toxicity of deoxynivalenoldol (deoxynivalenoldon) on hippocampal nerve cells of piglets, cultured hippocampal neurons in vitro to logarithmic growth stage, the cells were treated with don of different concentrations of 0 125 ng/m / L and 2 000 ng/m / L, respectively. The morphologic changes of cells were observed by inverted microscope and transmission electron microscope. The cell nucleus was labeled with Hoechest 33258 staining solution. The cell survival rate was detected by CCK-8 kit, and the leakage rate of LDH was detected by lactate dehydrogenase (LDH) kit. The results showed that compared with the control group, the cells in the treatment group had obvious morphological changes. With the increase of don concentration, cell density decreased, vacuolation, chromatin concentration and aggregation, and apoptotic bodies were produced. The survival rate of the cells in the treatment group was significantly decreased, and the leakage rate of LDH was increased with the increase of don concentration in a dose-and time-dependent manner. The difference was very significant when the concentration was above 500 ng/m / L compared with the control group (P0.01). The results showed that don had obvious toxic effect on hippocampal nerve cells of piglets and could induce cell damage and apoptosis.
【作者單位】： 安徽農(nóng)業(yè)大學(xué)動物科技學(xué)院;
【基金】：國家自然科學(xué)基金項目(31472250) 安徽省生豬產(chǎn)業(yè)技術(shù)體系資助項目(2016-2020)
【分類號】：S859.8

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