本文選題：羊腸道病毒 + CEV-JL��； 參考：《中國(guó)獸醫(yī)學(xué)報(bào)》2017年04期

【摘要】：應(yīng)用本實(shí)驗(yàn)室分離的國(guó)內(nèi)首株羊腸道病毒(CEV)-JL14VP1重組蛋白制備的兔源多克隆抗體和抗CEV-JL14病毒的單克隆抗體,建立了檢測(cè)CEV抗原的雙抗體夾心ELISA方法,并對(duì)吉林省和內(nèi)蒙古地區(qū)羊群感染CEV進(jìn)行了病原流行病學(xué)調(diào)查。方陣試驗(yàn)確定了CEV VP1鼠源IgG捕獲抗體的最佳包被量為0.2μg/孔,酶標(biāo)抗體的最佳稀釋倍數(shù)為1∶1 000。對(duì)大量CEV陰性糞便樣品進(jìn)行檢測(cè)與統(tǒng)計(jì)學(xué)處理,確定了雙抗體夾心ELISA檢測(cè)CEV的判定標(biāo)準(zhǔn)為樣品D490值≥0.216判定為陽(yáng)性。特異性、敏感性和重復(fù)性試驗(yàn)結(jié)果表明,所建立的檢測(cè)CEV抗原方法具有特異、敏感、快速和重復(fù)性好等特點(diǎn)。以建立的雙抗體夾心ELISA方法,對(duì)吉林省和內(nèi)蒙古不同地區(qū)的羊群糞便樣品進(jìn)行了檢測(cè),發(fā)現(xiàn)不同地區(qū)的羊群都存在著嚴(yán)重的CEV感染,感染率高達(dá)12%~100%。本研究為今后CEV感染的診斷、檢疫及防制提供了有效的手段和流行病學(xué)理論依據(jù)。
[Abstract]:Using rabbit polyclonal antibody and monoclonal antibody against CEV-JL14 virus, the first sheep enterovirus recombinant protein in China, a sandwich Elisa method for detecting CEV antigen was established by using rabbit polyclonal antibody and monoclonal antibody against CEV-JL14 virus. The pathogen epidemiology of sheep infection with CEV in Jilin province and Inner Mongolia was investigated. The best encapsulation of IgG capture antibody of CEV VP1 mouse source was 0.2 渭 g / well and the best dilution multiple of enzyme labeled antibody was 1:1 000 by square array test. A large number of CEV-negative fecal samples were detected and statistically analyzed. The standard of double antibody sandwich Elisa for CEV detection was determined to be that the sample D490 鈮，

本文編號(hào)：2047925