本文選題：C、D型產(chǎn)氣莢膜梭菌 + 氫氧化鋁佐劑��； 參考：《山東農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：產(chǎn)氣莢膜梭菌在自然界是無處不在的,是多種健康動物腸道的常在菌,能造成人及多種動物發(fā)病,且有發(fā)病急、病程短、死亡快的特點,每年大量動物因感染產(chǎn)氣莢膜梭菌而死亡,給養(yǎng)殖業(yè)造成巨大損失。C、D型產(chǎn)氣莢膜梭菌能單獨或混合感染牛羊等多種動物,引起動物壞死性腸炎及腸毒血癥,且兩型感染短時間內(nèi)難以區(qū)分。為了控制產(chǎn)氣莢膜梭菌引起的多種疾病,本試驗研制了C、D型產(chǎn)氣莢膜梭菌類毒素二價苗及抗毒素血清,有效預(yù)防并治療C、D型產(chǎn)氣莢膜梭菌病。隨著抗生素的廣泛應(yīng)用,細(xì)菌耐藥性逐漸增強,特別是產(chǎn)氣莢膜梭菌病一旦發(fā)生,抗生素難以治療,用產(chǎn)氣莢膜梭菌疫苗預(yù)防是最佳選擇。根據(jù)產(chǎn)氣莢膜梭菌致病是由其所產(chǎn)生外毒素引起的致病機理,本試驗研制了安全、高效的類毒素疫苗。疫苗效力往往與抗原濃度成正比,為了提高抗原濃度,本試驗選取實驗室最佳菌株,將C型菌與D型菌以4:3比例接種于同一培養(yǎng)基,高效產(chǎn)毒,測得毒素對小鼠的半數(shù)致死量2-5.8/mL,并用該毒素制備了產(chǎn)氣莢膜梭菌鋁膠苗和白油苗。我國現(xiàn)有產(chǎn)氣莢膜梭菌商品苗均為滅活鋁膠聯(lián)苗,免疫后發(fā)病時有發(fā)生,因此本試驗研制了產(chǎn)氣莢膜梭菌二價苗。為了證明疫苗的安全性和有效性,將兩種疫苗做安全性檢驗,測定外毒素對家兔的最小致死量,并使用不同劑量疫苗免疫家兔,21d后做攻毒保護(hù)試驗。結(jié)果表明:兩種疫苗免疫家兔,免疫部位均無潰瘍及肉芽腫,28d后剖檢免疫部位無殘留疫苗存在,兩種疫苗均安全性良好;C型毒素對家兔的最小致死量為0.04m L,D型毒素對家兔的最小致死量為0.4m L;兩種疫苗不同劑量免疫家兔,通過攻毒保護(hù)試驗,測得鋁膠苗對家兔的最小免疫劑量為2mL,白油類毒素苗對家兔的最小免疫劑量為1mL。產(chǎn)氣莢膜梭菌發(fā)病后用抗生素難以治療�？寡遄鳛橐环N快速的特異性治療制劑,能快速中和毒素,治療由C、D型產(chǎn)氣莢膜梭菌引起的中毒性疾病。本試驗使用高濃度毒素制備的油苗作為第一免疫原,除菌外毒素作為第二免疫原,制備高效價抗毒素血清,用間接ELISA法監(jiān)測抗體水平變化,并使用小鼠中和試驗檢驗抗血清的抗體水平。結(jié)果表明:本試驗制備的高效價C、D型產(chǎn)氣莢膜梭菌抗毒素血清,每0.1mL血清能中和400個C型毒素對小鼠的MLD和600個D型毒素MLD,有良好的應(yīng)用前景。
[Abstract]:Clostridium perfringens is ubiquitous in nature. It is a common bacteria in the intestinal tract of many healthy animals. It can make adults and many kinds of animals, and it has the characteristics of rapid onset, short course and fast death. A large number of animals die from the infection of Clostridium perfringens, causing huge loss of.C and D type Clostridium perfringens can be isolated or mixed. A variety of animals, such as cattle and sheep, can cause animal necrotizing enteritis and intestinal toxemia, and the type two infection is difficult to distinguish in a short time. In order to control a variety of diseases caused by Clostridium perfringens, this experiment has developed C, D type Clostridium perfringens toxoid and antitoxin serum, effective prevention and treatment of C, D type Clostridium perfringens disease. With resistance to resistance to Clostridium perfringens of type D. The antibiotic resistance of Clostridium perfringens is increasing, especially when Clostridium perfringens is occurring, antibiotics are difficult to treat, and Clostridium perfringens vaccine is the best choice. According to Clostridium perfringens, the pathogenic mechanism caused by the exotoxin produced by Clostridium perfringens is a safe and efficient vaccine. The potency is often proportional to the antigen concentration. In order to improve the antigen concentration, this experiment selects the best laboratory strain, inoculating C and D bacteria in the same medium with the same medium, producing the toxin efficiently and measuring half the lethal dose 2-5.8/mL of the mice, and using the toxin to prepare the aluminum and white oil production of Clostridium perfringens. In our country, the existing gas producing gas is produced. In order to prove the safety and effectiveness of the vaccine, the safety and effectiveness of the vaccine were tested and the minimum lethal dose of exotoxin to rabbits was measured in order to prove the safety and effectiveness of the vaccine, and the rabbits were immunized with different doses of vaccines and then attacked by 21d after 21d. The results showed that there were no ulcers and granulomatosis in the immune parts of the two vaccinated rabbits. After 28d, there was no residual vaccine in the immunization site, the two vaccines were all safe, the minimum lethal dose of C toxin to rabbits was 0.04m L, and the minimum lethal dose of D toxin to rabbits was 0.4m L; the two kinds of vaccines were immune to rabbits. The minimum immunization dose of aluminum gel seedlings to rabbits was 2mL, and the minimum immunization dose of white oil vaccine was 1mL. after the onset of Clostridium perfringens. The antiserum was a rapid and specific treatment preparation, which could quickly neutralize the toxin, and the treatment was caused by C and D type Clostridium perfringens. In this experiment, the oil vaccine prepared by high concentration toxin was used as the first immunogen, and the bacteria exotoxin was used as the second immunogen to prepare the high effective antitoxin serum. The antibody level was monitored by indirect ELISA, and the antibody level of the antiserum was tested by the neutralization test in mice. The results showed that the high effective price, C, D prepared by this experiment Clostridium perfringens antitoxin serum, which can neutralize 400 C toxin in each 0.1mL serum, has good application prospects for MLD and 600 D toxin MLD in mice.
【學(xué)位授予單位】：山東農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S852.4

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 曲海波;劉洪斌;白同臣;;A型產(chǎn)氣莢膜梭菌類毒素疫苗與全菌滅活疫苗的安全性和免疫效力的比較研究[J];中國獸藥雜志;2010年02期

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本文編號：2042583