發(fā)布時(shí)間：2018-06-18 20:35

  本文選題：中藥提取物復(fù)方制劑 + 新城疫病毒��； 參考：《西北農(nóng)林科技大學(xué)》2017年碩士論文

【摘要】：鴿新城疫(newcastle disease,ND)又稱鴿瘟或鴿Ⅰ型副粘病毒(pigeon paramyxovirus type 1,PPMV-1),是由新城疫病毒(newcastle disease virus,NDV)引起的一種急性、熱性、敗血性和高度接觸性傳染病。為預(yù)防和治療該疾病的發(fā)生,大量使用抗生素和化學(xué)類藥物,導(dǎo)致耐藥性和藥物殘留問(wèn)題的出現(xiàn)。而且隨著生態(tài)養(yǎng)殖和綠色食品等概念的提出,我國(guó)規(guī)定在飼料和食品中嚴(yán)禁使用獸醫(yī)臨床中抗生素類藥物和化學(xué)類藥物。中藥具有抗病毒作用效果好、毒副作用小和無(wú)耐藥性等優(yōu)點(diǎn),不但能提高機(jī)體免疫力,還能促進(jìn)生長(zhǎng),提高生產(chǎn)性能等。本研究在中獸醫(yī)藥理論指導(dǎo)下,以金銀花、黃芩、連翹、貫葉連翹等10余味中藥提取物進(jìn)行中藥提取物復(fù)方制劑,研究中藥提取物復(fù)方制劑的抗病毒能力、免疫增強(qiáng)能力和質(zhì)量控制標(biāo)準(zhǔn)。結(jié)果如下:1.中藥提取物復(fù)方制劑高劑量(含生藥2 g/mL)預(yù)防、治療組和中劑量(含生藥1 g/mL)預(yù)防、治療組的血凝效價(jià)顯著降低(P0.01),其中中藥提取物復(fù)方制劑中劑量組(含生藥1 g/m L)的效果顯著。2.中藥提取物復(fù)方制劑能夠提高NDV疫苗抗體效價(jià),中藥提取物復(fù)方制劑中劑量組(含生藥1 g/mL)的抗體效價(jià)差異顯著(P0.05);中藥提取物復(fù)方制劑中劑量組法氏囊器官指數(shù)差異顯著(P0.05);免疫器官內(nèi)淋巴細(xì)胞排列緊密,且淋巴細(xì)胞數(shù)量高于陽(yáng)性對(duì)照組。3.以黃芩苷為檢測(cè)成分,利用TLC和HPLC檢測(cè)方法對(duì)中藥提取物復(fù)方制劑進(jìn)行質(zhì)量控制研究。TLC結(jié)果顯示,黃芩苷供試品和標(biāo)準(zhǔn)品在相應(yīng)的位置上出現(xiàn)了顏色相同的斑點(diǎn),陰性對(duì)照品中無(wú)干擾現(xiàn)象;HPLC結(jié)果顯示,黃芩苷在濃度為10~200μg/mL之間時(shí)線性關(guān)系良好,黃芩苷的含量為3727.41 mg/g,在供試品中的黃芩苷峰保留時(shí)間與標(biāo)準(zhǔn)品中的峰是一致的,且陰性對(duì)照品無(wú)干擾現(xiàn)象。中藥提取物復(fù)方制劑對(duì)新城疫病毒具有較好的抑制作用,能夠顯著提高NDV疫苗抗體效價(jià)和法氏囊器官指數(shù),免疫器官內(nèi)淋巴細(xì)胞排列緊密且數(shù)量增多。所建立的黃芩苷TLC和HPLC檢測(cè)方法,專屬性強(qiáng),可快速準(zhǔn)確地對(duì)中藥提取物復(fù)方制劑中黃芩苷進(jìn)行檢測(cè)。
[Abstract]:Pigeon Newcastle disease (NDV), also known as pigeon plague or pigeon paramyxovirus type 1 (PPMV-1), is an acute, feverish, septic and highly contagious disease caused by Newcastle disease virus. In order to prevent and treat the disease, antibiotics and chemical drugs are used extensively, which leads to the emergence of drug resistance and drug residues. With the concept of ecological breeding and green food being put forward, the use of antibiotics and chemical drugs in veterinary clinic is strictly prohibited in feed and food in China. Traditional Chinese medicine has the advantages of good antiviral effect, little side effect and no drug resistance. It can not only improve the body immunity, but also promote growth, improve the production performance and so on. In this study, under the guidance of Chinese veterinary medicine theory, 10 aftertaste extracts such as Flos Lonicerae, Radix Scutellariae, Forsythia Forsythia and Hypericum perforatum were used to prepare Chinese medicine extract compound preparation, and the antiviral ability of compound preparation of traditional Chinese medicine extract compound preparation was studied. Immune enhancement and quality control standards. The result is as follows: 1. High dose (including crude drug 2 g / mL) prevention, treatment group and middle dose (containing crude drug 1 g / mL) prevention, The hemagglutination titer of the treatment group was significantly lower than that of the control group, and the effect of the middle dose group (containing 1 g / m L crude drug) was significant. The compound preparation of Chinese traditional medicine extract can improve the antibody titer of NDV vaccine. The antibody titers of the middle dose group (containing 1 g / mL crude drug) were significantly different from those of the middle dose group (P 0.05), the difference of the bursa organ index was significant (P 0.05), and the lymphocytes in the immune organs were closely arranged. The number of lymphocytes was higher than that of positive control group. Using baicalin as the detection component, the quality control of compound preparation of traditional Chinese medicine extract was studied by TLC and HPLC. The results showed that the same color spots appeared in the samples and standard samples of baicalin. The results of HPLC showed that baicalin had a good linear relationship when the concentration of baicalin was 10 渭 g / mL, and the content of baicalin was 3727.41 mg / g. The retention time of baicalin peak in the sample was the same as that in the standard sample. And negative reference substance has no interference phenomenon. The compound preparation of Chinese traditional medicine extract has a good inhibitory effect on Newcastle disease virus, can significantly improve the antibody titer of NDV vaccine and the bursal organ index of bursa, and the lymphocyte arrangement in immune organs is close and the number of lymphocytes increases. The established TLC and HPLC methods for the detection of baicalin can be used for rapid and accurate determination of baicalin in compound preparations of traditional Chinese medicine.
【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S853.7

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