本文選題：豬繁殖與呼吸綜合征 + ORF基因��； 參考：《中國病原生物學雜志》2017年03期

【摘要】：目的對廣西地區(qū)豬繁殖與呼吸綜合征病毒(PRRSV)Nsp2和ORF5基因進行分子流行病學調查。方法采集廣西地區(qū)豬病料樣品45份,PCR擴增PRRSV毒株Nsp2和ORF5基因并進行遺傳進化分析。結果 RT-PCR檢測PRRSV 7份豬病料陽性。7株病毒與VR-2332和LV株的同源性分別為83.5%88.7%和62.1%64.8%。遺傳進化分析顯示,7株病毒分屬于兩個亞群,3株屬于亞群Ⅳ,4株屬于亞群Ⅵ。Nsp2序列分析顯示,6株病毒有高致病性PRRSV 1+29aa氨基酸的缺失特征,其中GXBB11-2015在此基礎上出現(xiàn)了新的20個氨基酸缺失,缺失堿基數(shù)為150個堿基。結論高致病性PRRSV已成為廣西地區(qū)優(yōu)勢毒株,病毒Nsp2基因新的堿基缺失是PRRSV變異的又一證據(jù),這為研究該部位基因缺失對PRRSV的影響打下了基礎
[Abstract]:Objective to investigate the molecular epidemiology of porcine reproductive and respiratory syndrome virus (PRRSVV) Nsp2 and ORF5 genes in Guangxi. Methods Nsp2 and ORF5 genes of PRRSv strains were amplified by PCR from 45 samples of pig disease in Guangxi and genetic evolution analysis was carried out. Results the homology of PRRSv positive 7 strains with VR-2332 and LV was 83.5.7% and 62.1 d.8respectively. Genetic evolution analysis showed that 7 viruses belong to two subgroups, 3 belong to subgroup 鈪，

本文編號：2028827