本文選題：杜民雜交豬 + 微衛(wèi)星標(biāo)記��； 參考：《東北農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：本研究采用世界糧農(nóng)組織和國際動物遺傳學(xué)會聯(lián)合推薦的25個微衛(wèi)星標(biāo)記,對不同世代杜民豬進行了基因雜合度的遺傳學(xué)檢測,同時測定了產(chǎn)仔數(shù)、產(chǎn)活仔數(shù)、21日齡活仔數(shù)、初生重、初生窩重、21日齡重、21日齡窩重、35日齡重、35日齡窩重、哺乳期日增重及育成率等經(jīng)濟性狀,并檢測了試驗豬的血清、乳清Ig G含量。通過對基因雜合度的世代變化及其與繁殖性狀的相關(guān)分析,獲得如下研究結(jié)果:(1)微衛(wèi)星標(biāo)記在杜民雜交豬DM、DM1、DM2、DM3中均表現(xiàn)出多態(tài)性,25個標(biāo)記座位上檢測到的等位基因數(shù)在2~8之間,SW24座位上的等位基因最多,SW1828、S0227、S0143座位上的等位基因最少。四個世代中檢測到的等位基因總數(shù)分別為114、106、105、87個,平均有效等位基因數(shù)分別為3.2927、3.0405、3.2924、2.6513。試驗豬中檢測到的等位基因頻率范圍為0.0238~0.9359,多數(shù)等位基因頻率大于0.05。(2)四個世代的群體平均基因雜合度逐世代降低,分別為0.6703、0.6425、0.6238、0.5924,DM平均基因雜合度顯著高于DM3(P0.05);檢測群體的多態(tài)信息含量(PIC)范圍為0.2205~0.9394,僅有5個標(biāo)記的PIC小于0.5,而四個群體的平均多態(tài)信息含量均大于0.5,呈現(xiàn)高度多態(tài)。(3)DM各繁殖性狀值高于DM1,并顯著高于DM2(P0.05)。DM的哺乳期日增重和血清Ig G水平高于其它世代;DM1的初生重、初生窩重、21日齡重、21日齡窩重、21日齡活仔數(shù)、35日齡窩重顯著高于DM2(P0.05);DM、DM1、DM2的仔豬育成率和乳清Ig G含量在各世代間無顯著差異。(4)將個體基因雜合度以0.1為步長分為5個等級,DM個體基因雜合度主要分布于水平3~5,水平5的個體數(shù)最多;DM1與DM2個體基因雜合度均主要分布于水平2~4,水平3的個體數(shù)最多,但DM1處于水平4的個體多于DM2;DM3個體基因雜合度主要分布于水平1~3,水平3的個體數(shù)最多。(5)產(chǎn)仔數(shù)、產(chǎn)活仔數(shù)、21日齡活仔數(shù)均隨基因雜合度的增高而增大;基因雜合度與產(chǎn)仔數(shù)、產(chǎn)活仔數(shù)、初生窩重、21日齡活仔數(shù)、哺乳期日增重之間顯著正相關(guān)(P0.05),而與初生重、21日齡重、21日齡窩重、35日齡重、35日齡窩重、育成率及血清Ig G含量相關(guān)不顯著。
[Abstract]:In this study, 25 microsatellite markers recommended by the World Food and Agriculture Organization and the International Society for Animal Genetics were used to detect genetic heterozygosity of different generations of Du Min pigs. Birth weight, litter weight at 21 days old, litter weight at 35 days old and litter weight at 35 days old, daily gain during lactation and breeding rate, and so on. The serum and IGG content of whey were measured. By analyzing the generation variation of heterozygosity and its correlation with reproductive traits, The results were as follows: (1) the microsatellite markers showed polymorphism in Du Min crossbred pig DM1, DM2DM2DM3. The number of alleles detected in 25 marker loci ranged from 2 to 8. The highest number of alleles detected on SW24 loci were SW1828 and S0227S0143. The total number of alleles detected in the four generations were 114106n105,87, and the average effective alleles were 3.29273.04053.2924n2.6513. The frequency range of alleles detected in the tested pigs was 0.0238U 0.9359, and the average heterozygosity of the four generations of the population was decreased from generation to generation, and the frequency of most alleles was greater than 0.05.2in the four generations. The average heterozygosity of DM was significantly higher than that of DM3P 0.05N, and the range of polymorphic information content was 0.22050.9394.The PIC of only 5 markers was less than 0.5, while the average polymorphism information content of the four populations was higher than 0.5, showing high polymorphism. The value of colonization traits was higher than that of DM1, and significantly higher than that of DM _ 2P _ (0.05N) 路DM during lactation period, and the level of serum IgG was higher than that of other generations. Litter weight at 21 days old / 21 days old litter weight at 21 days old / 35 days old litter weight was significantly higher than that in the piglets with DM2P0.05 / DM1 / DM2. There was no significant difference in the percentage of reproductive capacity and the content of IGG in whey between generations. 4) the heterozygosity of individual genes was divided into 5 groups with a step of 0.1. The heterozygosity of DM individuals was mainly distributed at level 3 and 5, and the highest number of individuals in level 5 was found in both DM1 and DM2 individuals. The heterozygosity of individuals in level 3 was the highest in level 2. However, the heterozygosity of DM1 at level 4 was higher than that of DM2DM3, and the heterozygosity of individuals in level 3 was the highest at level 1, and the number of live litter at level 3 increased with the increase of heterozygosity, and the heterozygosity of gene and litter size increased with the increase of heterozygosity. There was a significant positive correlation between litter size, litter weight and daily gain during lactation, but there was no significant correlation between litter size and litter weight at 21 day old, litter weight at 35 day old and serum IgG content.
【學(xué)位授予單位】：東北農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S828

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本文編號：2022847