發(fā)布時(shí)間：2018-06-15 13:39

  本文選題：PRRSV + Viperin��； 參考：《病毒學(xué)報(bào)》2017年02期

【摘要】：為了研究干擾素刺激基因Viperin對(duì)豬繁殖與呼吸綜合征病毒的抗病毒作用,本研究構(gòu)建了豬源Viperin基因(sViperin)pCI-sVIP表達(dá)載體,并在MARC-145細(xì)胞上觀察了其對(duì)PRRSV復(fù)制的抑制作用。結(jié)果為:過表達(dá)sViperin蛋白可以抑制PRRSV在MARC-145細(xì)胞上的復(fù)制,且具有劑量依賴作用。sViperin蛋白抑制了病毒的入胞,但其對(duì)病毒的裝配和釋放沒有影響作用。共聚焦實(shí)驗(yàn)證明sViperin蛋白主要定位于內(nèi)質(zhì)網(wǎng)等細(xì)胞器。sViperin蛋白和PRRSV GP5、N蛋白在細(xì)胞內(nèi)存在共定位現(xiàn)象。CO-IP實(shí)驗(yàn)證明sViperin蛋白可以和PRRSV N蛋白存在相互作用。該研究為該病毒新型抗病毒藥物研究奠定了重要基礎(chǔ)。
[Abstract]:In order to study the antiviral effect of interferon stimulating gene Viperin on porcine reproductive and respiratory syndrome virus (PRRSv), a porcine Viperin gene vector was constructed and its inhibitory effect on PRRSv replication was observed in MARC-145 cells. The results showed that the overexpression of sViperin protein could inhibit the replication of PRRSv in MARC-145 cells, and in a dose-dependent manner. SViperin protein inhibited the entry of the virus, but it had no effect on the assembly and release of the virus. Confocal assay showed that sViperin protein was mainly located in endoplasmic reticulum. SViperin protein and PRRSV GP5N protein were colocated in cells. CO-IP experiment showed that sViperin protein could interact with PRRSv N protein. This study has laid an important foundation for the study of new antiviral drugs.
【作者單位】： 南京農(nóng)業(yè)大學(xué)動(dòng)物醫(yī)學(xué)院;河南省農(nóng)業(yè)科學(xué)院畜牧獸醫(yī)研究所;普萊柯生物工程股份有限公司;
【基金】：國家自然科學(xué)基金(項(xiàng)目號(hào):31672565),題目:TGF-β信號(hào)通路在豬繁殖與呼吸綜合征病毒和副豬嗜血桿菌協(xié)同感染中的作用 國家生豬產(chǎn)業(yè)技術(shù)體系專項(xiàng)(項(xiàng)目號(hào):CARS-36)
【分類號(hào)】：S852.651
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本文編號(hào)：2022183