本文選題：巴馬香豬 + 皮膚厚度��； 參考：《中國(guó)農(nóng)業(yè)科學(xué)》2016年16期

【摘要】：【目的】測(cè)定中國(guó)地方小型豬品種巴馬香豬成年時(shí)周身9個(gè)典型部位的皮膚厚度,揭示巴馬香豬不同部位皮膚厚度變化規(guī)律,進(jìn)行9個(gè)部位皮膚厚度與候選SNPs位點(diǎn)的關(guān)聯(lián)分析,在巴馬香豬群體中驗(yàn)證影響皮膚厚度的7號(hào)染色體主效QTL,為進(jìn)一步在巴馬香豬群體中大規(guī)模開(kāi)展皮膚厚度等形態(tài)變化的分子遺傳控制機(jī)理及其相關(guān)基因功能研究奠定基礎(chǔ),從而增強(qiáng)人們對(duì)豬皮膚的認(rèn)知�！痉椒ā繌囊粋�(gè)由319頭300日齡巴馬香豬組成的成年屠宰群體中,隨機(jī)選取50頭,包括27頭母豬和23頭閹割公豬,分別取頭臉、肩、背、肷、臀、胸、下腹、腋下和管等9個(gè)部位的皮膚,利用電子游標(biāo)卡尺對(duì)這些不同部位皮膚厚度進(jìn)行精確測(cè)量,利用R語(yǔ)言基本統(tǒng)計(jì)包進(jìn)行不同部位和不同性別間皮膚厚度的差異分析以及不同部位間皮膚厚度的相關(guān)分析。在豬7號(hào)染色體34.5-36.2Mb的區(qū)域選取46個(gè)SNPs位點(diǎn),利用MassARRAY時(shí)間飛行質(zhì)譜技術(shù)進(jìn)行基因分型,結(jié)合上述測(cè)定的皮厚表型,利用廣義混合線性模型及R語(yǔ)言SNPassoc軟件包進(jìn)行目標(biāo)候選區(qū)域的關(guān)聯(lián)分析。根據(jù)關(guān)聯(lián)分析結(jié)果和基因的生物學(xué)功能確定可能的位置候選基因�！窘Y(jié)果】(1)單因素方差分析表明巴馬香豬9個(gè)部位的皮膚厚度存在極顯著差異(P=2.95×10~(-117)),肷部和背部的皮膚最厚,分別為(5.15±0.92)和(4.97±0.85)mm,下腹和腋下的皮膚最薄,分別為(1.77±0.36)和(1.97±0.68)mm。皮膚厚度從厚到薄依次是肷部、背部、肩部、頭臉、臀部、管部、胸部、腋下和下腹。(2)閹割公豬腋下皮膚厚度顯著小于母豬的(P=0.021),其它部位皮膚厚度在母豬和閹割公豬間差異均不顯著。(3)除了下腹皮膚厚度與背部、肩部、頭臉部的不相關(guān)(P0.05)外,巴馬香豬不同部位兩兩之間皮膚厚度均呈現(xiàn)不同程度地顯著或極顯著正相關(guān)。(4)關(guān)聯(lián)分析結(jié)果表明,9個(gè)不同部位的皮膚厚度表型均與候選區(qū)域的某些SNPs存在極顯著的關(guān)聯(lián),從強(qiáng)到弱依次是肷部、肩部、背部、腋下、臀部、胸部、頭臉、管部和下腹。從而,證實(shí)了巴馬香豬群體存在影響豬皮膚厚度的7號(hào)染色體主效QTL。(5)3個(gè)與皮膚厚度關(guān)聯(lián)性最強(qiáng)的SNPs值得進(jìn)一步關(guān)注,分別位于7號(hào)染色體的34856565、35543837和35573869位置。肷部的皮膚厚度與SNP(chr7:34856565)的關(guān)聯(lián)顯著性最強(qiáng)(P_(cor)=5.15×10~(-6)),這個(gè)SNP也是肩部皮膚厚度最關(guān)聯(lián)(P_(cor)=5.75×10~(-6))的位點(diǎn)。SNP(chr7:35543837)是腋下(P_(cor)=3.05×10~(-5))、臀部(P_(cor)=0.010)、胸部(P_(cor)=0.013)和頭臉(P_(cor)=0.025)皮膚厚度的最關(guān)聯(lián)位點(diǎn),也是肩部皮膚厚度的次最關(guān)聯(lián)位點(diǎn)。SNP(chr7:35573869)則是背部皮膚厚度的最關(guān)聯(lián)位點(diǎn)(P_(cor)=1.17×10~(-5)),SNPs(chr7:35543837和chr7:34856565)次之。(6)根據(jù)最強(qiáng)關(guān)聯(lián)SNPs所在基因及基因生物學(xué)功能,初步推測(cè)ANKS1A和HMGA1基因可能是影響皮膚厚度的候選因果基因�！窘Y(jié)論】較全面地測(cè)量了中國(guó)小型地方豬品種巴馬香豬周身皮膚厚度,揭示了巴馬香豬皮膚厚度在不同部位之間的變化規(guī)律。在巴馬香豬群體中驗(yàn)證了影響皮膚厚度的7號(hào)染色體主效QTL位點(diǎn),肷部、背部和肩部皮膚厚度表型性狀與候選SNPs位點(diǎn)關(guān)聯(lián)性更強(qiáng),可能適合下一步大規(guī)模深入分析。ANKS1A和HMGA1基因可能是影響皮膚厚度的候選因果基因,但需要進(jìn)一步生物學(xué)功能試驗(yàn)證明。
[Abstract]:[Objective] to determine the skin thickness of 9 typical parts of the body of Bama Xiang pig in Chinese local miniature pig, to reveal the skin thickness variation in different parts of Bama Xiang pig and to analyze the correlation between the skin thickness of 9 parts and the candidate SNPs loci, and to verify the main effect of the main effect QTL of chromosome 7 on the skin thickness in Bama Xiang pig population. In addition, the molecular genetic control mechanism and related gene function of the skin thickness changes in Bama pig population are further established, thus enhancing people's cognition of pig skin. [Methods] 50 heads, including 27 mothers, were randomly selected from a adult slaughterhouse composed of 319 Bama pigs and 300 days old. Pigs and 23 castrated boars, respectively, take the skin of 9 parts of the head, shoulder, back, shoulder, back, hip, chest, lower abdomen, axillary and tube, and use the electronic vernier caliper to measure the skin thickness of these different parts accurately, and use the basic statistics package of R language to analyze the difference of skin thickness between different parts and different sexes and the skin thickness between different parts. Correlation analysis of degree. 46 SNPs loci were selected in the region of 34.5-36.2Mb of chromosome 7 of the pig. MassARRAY time flight mass spectrometry was used to genotyping, combined with the skin thickness phenotype measured above, the association analysis of the target candidate region was carried out by the generalized mixed linear model and the R language SNPassoc software package. The possible location candidate genes were determined by the biological function. [results] (1) one factor variance analysis showed that the skin thickness of the 9 parts of Bama Xiang pig was very significant (P=2.95 x 10~ (-117)). The skin was the thickest, (5.15 + 0.92) and (4.97 + 0.85) mm respectively, and the skin of lower abdomen and underarm was the thinnest, respectively (1.77 + 0.36). And (1.97 + 0.68) mm. skin thickness from thick to thin in turn, back, shoulder, head, face, hip, tube, chest, axillary and lower abdomen. (2) the thickness of axillary skin in castration boars was significantly smaller than sow (P=0.021), and the skin thickness of other parts was not significant between sows and castrated male pigs. (3) the thickness of the lower abdomen and the back, shoulder, head, and head were not significant. The skin thickness of 22 different parts of the Bama pig was significantly or significantly positively correlated with 22 of the different parts of the Bama Xiang pig. (4) the correlation analysis showed that the skin thickness phenotypes of the 9 different parts were closely related to some of the SNPs in the candidate regions, from the strong to the weak, in turn, in the shoulder, back, and under the armpit. Hip, chest, head, face, tube and lower abdomen. Thus, it was confirmed that the 7 main QTL. (5) of the Bama Xiang pig population affected the thickness of the skin of the pig (5), 3 of the strongest association with the thickness of the skin deserve further attention, located at 3485656535543837 and 35573869 of chromosome 7, respectively. Skin thickness and SNP (chr7:34856565). The strongest association (P_ (COR) =5.15 x 10~ (-6)) is the most associated site of the skin thickness of the shoulder (P_ (COR) =5.75 x 10~ (-6)), the most associated site of the skin thickness in the buttocks, and the skin thickness of the shoulder. The most associated site.SNP (chr7:35573869) is the most associated site of the skin thickness of the back (P_ (COR) =1.17 * 10~ (-5)), SNPs (chr7:35543837 and chr7:34856565). (6) according to the gene and the biological function of the strongest association SNPs gene and gene, it is preliminarily presumed that ANKS1A and genes may be candidate causal genes affecting the thickness of the skin. The skin thickness of Bama Xiang pig was measured in a small local pig breed in China, and the changes in the skin thickness of Bama Xiang pig were revealed in different parts. The main effect QTL loci of chromosome 7, the skin thickness of the back and shoulder, and the candidate SNPs loci of the skin thickness of the Bama Xiang pig population were verified in the Bama pig population. More relevant, it may be suitable for the next large-scale in-depth analysis of the.ANKS1A and HMGA1 genes may be candidate causal genes affecting the skin thickness, but further biological functional tests are required.
【作者單位】： 江西農(nóng)業(yè)大學(xué)省部共建豬遺傳改良與養(yǎng)殖技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金(31460283)
【分類(lèi)號(hào)】：S828

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王占元;袁淑芹;盧景都;祝云江;;巴馬香豬寒地?cái)U(kuò)繁效益分析[J];養(yǎng)殖技術(shù)顧問(wèn);2010年02期

2 周澤平;;巴馬香豬[J];湖南農(nóng)業(yè);2013年08期

3 魯春霞;袁淑芹;王占元;徐占東;;巴馬香豬的引進(jìn)與飼養(yǎng)[J];養(yǎng)殖技術(shù)顧問(wèn);2010年02期

4 魯春霞;祝云江;楊曉明;袁淑芹;;北方寒地引進(jìn)繁育巴馬香豬的工作及其效果[J];養(yǎng)殖技術(shù)顧問(wèn);2010年03期

5 孫強(qiáng)東;;巴馬香豬閹割技術(shù)及其注意事項(xiàng)[J];養(yǎng)殖與飼料;2011年07期

6 王愛(ài)德，，蘭干球，郭亞芬;巴馬香豬耐熱性的探討[J];家畜生態(tài);1995年04期

7 張自勤;長(zhǎng)壽之鄉(xiāng)的巴馬香豬[J];中國(guó)牧業(yè)通訊;1998年05期

8 張承露;曾德軍;趙貴軍;;巴馬香豬耐熱性解析[J];湖北畜牧獸醫(yī);2014年02期

9 ;廣西巴馬香豬[J];科技致富向?qū)?2009年03期

10 劉偉;;能拎著走的烤全豬——巴馬香豬[J];農(nóng)業(yè)知識(shí);2012年07期

相關(guān)會(huì)議論文 前3條

1 孔德?tīng)I(yíng);;通過(guò)正交實(shí)驗(yàn)設(shè)計(jì)優(yōu)化巴馬香豬精液凍存方法[A];中國(guó)畜牧獸醫(yī)學(xué)會(huì)——第三屆中國(guó)獸醫(yī)臨床大會(huì)論文集[C];2012年

2 張小杰;曾俊棋;王麗娟;朱誠(chéng)其;岳萬(wàn)福;;高通量16S測(cè)序法比較不同食性豬腸道細(xì)菌結(jié)構(gòu)差異[A];浙江省動(dòng)物學(xué)會(huì)第十二屆會(huì)員代表大會(huì)暨學(xué)術(shù)討論會(huì)論文摘要集[C];2013年

3 徐漢進(jìn);雍艷紅;巨向紅;趙云濤;安立龍;效梅;許英梅;;SYBR GreenⅠ實(shí)時(shí)熒光定量PCR檢測(cè)巴馬香豬Toll樣受體2 mRNA方法的建立[A];中國(guó)畜牧獸醫(yī)學(xué)會(huì)獸醫(yī)產(chǎn)科學(xué)分會(huì)第五屆全體會(huì)議第十次學(xué)術(shù)研討會(huì)論文集[C];2009年

相關(guān)重要報(bào)紙文章 前3條

1 本報(bào)記者 王銘澤　于立兵 通訊員 周兵;女礦老板迷上飼養(yǎng)巴馬香豬[N];中國(guó)特產(chǎn)報(bào);2010年

2 楊明遠(yuǎn)　記者 簡(jiǎn)放鵬;“巴馬香豬”落戶天仙示范片[N];瀘州日?qǐng)?bào);2010年

3 記者 王敏　吳德群;基因“迷你豬”打入寵物市場(chǎng)[N];深圳特區(qū)報(bào);2011年

相關(guān)博士學(xué)位論文 前1條

1 江雯;皮膚靶向表達(dá)hCTLA-4Ig轉(zhuǎn)基因巴馬香豬種群建立及其異種移植研究[D];第三軍醫(yī)大學(xué);2012年

相關(guān)碩士學(xué)位論文 前9條

1 段超偉;實(shí)驗(yàn)用巴馬香豬繁殖性能與仔豬生長(zhǎng)發(fā)育性能測(cè)定[D];西南大學(xué);2012年

2 陳玉龍;巴馬香豬肝硬化模型的建立及其腸道菌群演替的研究[D];西南大學(xué);2010年

3 敖秋桅;骨骼肌肌纖維類(lèi)型與巴馬香豬肉質(zhì)性狀的相關(guān)性研究[D];廣西大學(xué);2014年

4 宋玉東;巴馬香豬肝硬化形成過(guò)程中細(xì)菌移位的初步研究[D];西南大學(xué);2010年

5 葛亮;胃轉(zhuǎn)流手術(shù)對(duì)巴馬香豬腸道菌群及部分血清學(xué)指標(biāo)的影響[D];西南大學(xué);2013年

6 李亮;巴馬香豬血液生理生化指標(biāo)參考標(biāo)準(zhǔn)的建立及ENU誘變下血液指標(biāo)異常個(gè)體的篩選[D];第三軍醫(yī)大學(xué);2013年

7 何凌云;不同日糧對(duì)長(zhǎng)白豬和巴馬香豬細(xì)胞因子與激素水平的影響[D];湖南農(nóng)業(yè)大學(xué);2014年

8 丁浩;大豆異黃酮對(duì)巴馬香豬子宮和卵巢變化及其IGF家族基因表達(dá)的影響[D];湖南農(nóng)業(yè)大學(xué);2014年

9 吳群;公豬體內(nèi)主要膻味基因的表達(dá)分析研究[D];西南大學(xué);2013年

本文編號(hào)：2001038