本文選題：巴馬香豬 + 皮膚厚度�。� 參考：《中國農業(yè)科學》2016年16期

【摘要】：【目的】測定中國地方小型豬品種巴馬香豬成年時周身9個典型部位的皮膚厚度,揭示巴馬香豬不同部位皮膚厚度變化規(guī)律,進行9個部位皮膚厚度與候選SNPs位點的關聯(lián)分析,在巴馬香豬群體中驗證影響皮膚厚度的7號染色體主效QTL,為進一步在巴馬香豬群體中大規(guī)模開展皮膚厚度等形態(tài)變化的分子遺傳控制機理及其相關基因功能研究奠定基礎,從而增強人們對豬皮膚的認知。【方法】從一個由319頭300日齡巴馬香豬組成的成年屠宰群體中,隨機選取50頭,包括27頭母豬和23頭閹割公豬,分別取頭臉、肩、背、肷、臀、胸、下腹、腋下和管等9個部位的皮膚,利用電子游標卡尺對這些不同部位皮膚厚度進行精確測量,利用R語言基本統(tǒng)計包進行不同部位和不同性別間皮膚厚度的差異分析以及不同部位間皮膚厚度的相關分析。在豬7號染色體34.5-36.2Mb的區(qū)域選取46個SNPs位點,利用MassARRAY時間飛行質譜技術進行基因分型,結合上述測定的皮厚表型,利用廣義混合線性模型及R語言SNPassoc軟件包進行目標候選區(qū)域的關聯(lián)分析。根據關聯(lián)分析結果和基因的生物學功能確定可能的位置候選基因。【結果】(1)單因素方差分析表明巴馬香豬9個部位的皮膚厚度存在極顯著差異(P=2.95×10~(-117)),肷部和背部的皮膚最厚,分別為(5.15±0.92)和(4.97±0.85)mm,下腹和腋下的皮膚最薄,分別為(1.77±0.36)和(1.97±0.68)mm。皮膚厚度從厚到薄依次是肷部、背部、肩部、頭臉、臀部、管部、胸部、腋下和下腹。(2)閹割公豬腋下皮膚厚度顯著小于母豬的(P=0.021),其它部位皮膚厚度在母豬和閹割公豬間差異均不顯著。(3)除了下腹皮膚厚度與背部、肩部、頭臉部的不相關(P0.05)外,巴馬香豬不同部位兩兩之間皮膚厚度均呈現不同程度地顯著或極顯著正相關。(4)關聯(lián)分析結果表明,9個不同部位的皮膚厚度表型均與候選區(qū)域的某些SNPs存在極顯著的關聯(lián),從強到弱依次是肷部、肩部、背部、腋下、臀部、胸部、頭臉、管部和下腹。從而,證實了巴馬香豬群體存在影響豬皮膚厚度的7號染色體主效QTL。(5)3個與皮膚厚度關聯(lián)性最強的SNPs值得進一步關注,分別位于7號染色體的34856565、35543837和35573869位置。肷部的皮膚厚度與SNP(chr7:34856565)的關聯(lián)顯著性最強(P_(cor)=5.15×10~(-6)),這個SNP也是肩部皮膚厚度最關聯(lián)(P_(cor)=5.75×10~(-6))的位點。SNP(chr7:35543837)是腋下(P_(cor)=3.05×10~(-5))、臀部(P_(cor)=0.010)、胸部(P_(cor)=0.013)和頭臉(P_(cor)=0.025)皮膚厚度的最關聯(lián)位點,也是肩部皮膚厚度的次最關聯(lián)位點。SNP(chr7:35573869)則是背部皮膚厚度的最關聯(lián)位點(P_(cor)=1.17×10~(-5)),SNPs(chr7:35543837和chr7:34856565)次之。(6)根據最強關聯(lián)SNPs所在基因及基因生物學功能,初步推測ANKS1A和HMGA1基因可能是影響皮膚厚度的候選因果基因�！窘Y論】較全面地測量了中國小型地方豬品種巴馬香豬周身皮膚厚度,揭示了巴馬香豬皮膚厚度在不同部位之間的變化規(guī)律。在巴馬香豬群體中驗證了影響皮膚厚度的7號染色體主效QTL位點,肷部、背部和肩部皮膚厚度表型性狀與候選SNPs位點關聯(lián)性更強,可能適合下一步大規(guī)模深入分析。ANKS1A和HMGA1基因可能是影響皮膚厚度的候選因果基因,但需要進一步生物學功能試驗證明。
[Abstract]:[Objective] to determine the skin thickness of 9 typical parts of the body of Bama Xiang pig in Chinese local miniature pig, to reveal the skin thickness variation in different parts of Bama Xiang pig and to analyze the correlation between the skin thickness of 9 parts and the candidate SNPs loci, and to verify the main effect of the main effect QTL of chromosome 7 on the skin thickness in Bama Xiang pig population. In addition, the molecular genetic control mechanism and related gene function of the skin thickness changes in Bama pig population are further established, thus enhancing people's cognition of pig skin. [Methods] 50 heads, including 27 mothers, were randomly selected from a adult slaughterhouse composed of 319 Bama pigs and 300 days old. Pigs and 23 castrated boars, respectively, take the skin of 9 parts of the head, shoulder, back, shoulder, back, hip, chest, lower abdomen, axillary and tube, and use the electronic vernier caliper to measure the skin thickness of these different parts accurately, and use the basic statistics package of R language to analyze the difference of skin thickness between different parts and different sexes and the skin thickness between different parts. Correlation analysis of degree. 46 SNPs loci were selected in the region of 34.5-36.2Mb of chromosome 7 of the pig. MassARRAY time flight mass spectrometry was used to genotyping, combined with the skin thickness phenotype measured above, the association analysis of the target candidate region was carried out by the generalized mixed linear model and the R language SNPassoc software package. The possible location candidate genes were determined by the biological function. [results] (1) one factor variance analysis showed that the skin thickness of the 9 parts of Bama Xiang pig was very significant (P=2.95 x 10~ (-117)). The skin was the thickest, (5.15 + 0.92) and (4.97 + 0.85) mm respectively, and the skin of lower abdomen and underarm was the thinnest, respectively (1.77 + 0.36). And (1.97 + 0.68) mm. skin thickness from thick to thin in turn, back, shoulder, head, face, hip, tube, chest, axillary and lower abdomen. (2) the thickness of axillary skin in castration boars was significantly smaller than sow (P=0.021), and the skin thickness of other parts was not significant between sows and castrated male pigs. (3) the thickness of the lower abdomen and the back, shoulder, head, and head were not significant. The skin thickness of 22 different parts of the Bama pig was significantly or significantly positively correlated with 22 of the different parts of the Bama Xiang pig. (4) the correlation analysis showed that the skin thickness phenotypes of the 9 different parts were closely related to some of the SNPs in the candidate regions, from the strong to the weak, in turn, in the shoulder, back, and under the armpit. Hip, chest, head, face, tube and lower abdomen. Thus, it was confirmed that the 7 main QTL. (5) of the Bama Xiang pig population affected the thickness of the skin of the pig (5), 3 of the strongest association with the thickness of the skin deserve further attention, located at 3485656535543837 and 35573869 of chromosome 7, respectively. Skin thickness and SNP (chr7:34856565). The strongest association (P_ (COR) =5.15 x 10~ (-6)) is the most associated site of the skin thickness of the shoulder (P_ (COR) =5.75 x 10~ (-6)), the most associated site of the skin thickness in the buttocks, and the skin thickness of the shoulder. The most associated site.SNP (chr7:35573869) is the most associated site of the skin thickness of the back (P_ (COR) =1.17 * 10~ (-5)), SNPs (chr7:35543837 and chr7:34856565). (6) according to the gene and the biological function of the strongest association SNPs gene and gene, it is preliminarily presumed that ANKS1A and genes may be candidate causal genes affecting the thickness of the skin. The skin thickness of Bama Xiang pig was measured in a small local pig breed in China, and the changes in the skin thickness of Bama Xiang pig were revealed in different parts. The main effect QTL loci of chromosome 7, the skin thickness of the back and shoulder, and the candidate SNPs loci of the skin thickness of the Bama Xiang pig population were verified in the Bama pig population. More relevant, it may be suitable for the next large-scale in-depth analysis of the.ANKS1A and HMGA1 genes may be candidate causal genes affecting the skin thickness, but further biological functional tests are required.
【作者單位】： 江西農業(yè)大學省部共建豬遺傳改良與養(yǎng)殖技術國家重點實驗室;
【基金】：國家自然科學基金(31460283)
【分類號】：S828

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