本文選題：綿羊 + Lpin基因�。� 參考：《中國(guó)畜牧獸醫(yī)》2017年04期

【摘要】：試驗(yàn)旨在研究Lpin2基因遺傳變異對(duì)綿羊尾型和屠宰性狀的影響。以兩品種脂尾型綿羊廣靈大尾羊和小尾寒羊?yàn)檠芯繉?duì)象,采用DNA直接測(cè)序法檢測(cè)Lpin2基因5′非編碼區(qū)部分序列的單核苷酸多態(tài)性(SNPs),并分析其與尾型及屠宰性狀的關(guān)聯(lián)性。結(jié)果發(fā)現(xiàn),Lpin2基因5′非編碼區(qū)起始密碼子上游約1 200bp的DNA序列中存在3個(gè)SNPs,即NC_019480.2:g.-663dup ATT(SNP1)、g.-388TC(SNP2)和g.-330TG(SNP3),其中SNP1為三核苷酸ATT重復(fù)擴(kuò)展短串聯(lián)重復(fù)序列(STR)的拷貝數(shù)變異(CNV),在廣靈大尾羊中的突變頻率顯著高于小尾寒羊(P0.05),顯著降低了廣靈大尾羊的胴體重與屠宰率(P0.05),對(duì)小尾寒羊的屠宰性狀無(wú)顯著影響(P0.05)。SNP2和SNP3構(gòu)成單倍型塊,形成的單倍型對(duì)各性狀無(wú)顯著影響(P0.05)。試驗(yàn)結(jié)果可為綿羊肉質(zhì)性狀的標(biāo)記輔助選擇提供參考依據(jù)。
[Abstract]:The purpose of the experiment was to study the effect of genetic variation of Lpin2 gene on tail type and slaughter traits of sheep. The single nucleotide polymorphisms (SNPs) of the 5'non-coding region of Lpin2 gene were detected by direct DNA sequencing in two breeds of fat-tailed sheep, Guangling large tail sheep and small tail Han sheep, and their association with tail type and slaughter traits were analyzed. The results showed that there were three SNPs in the 5 'noncoding region of the Lpin2 gene, i.e., NC019480.2: g. -663DUTP SNP1 / SNP2) and SNP2 (g.-330TGGN SNP3), in which SNP1 was a trinucleotide ATT repeat extension short tandem repeat (STR). The mutation frequency in the tail sheep was significantly higher than that in the small tail Han sheep (P 0.05), which significantly decreased the carcass weight and slaughter rate (P 0.05) of the large tail sheep, and had no significant effect on the slaughter traits of the small tail Han sheep (P 0.05 SNP2 and SNP3). The haplotypes formed had no significant effect on each trait (P0.05). The results can provide reference for marker-assisted selection of meat quality traits in sheep.
【作者單位】： 山西農(nóng)業(yè)大學(xué)動(dòng)物科技學(xué)院;天津農(nóng)學(xué)院動(dòng)物科學(xué)與動(dòng)物醫(yī)學(xué)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(31372292) 天津農(nóng)學(xué)院科技發(fā)展基金(2013No1)
【分類(lèi)號(hào)】：S826
，

本文編號(hào)：1997162