本文選題：線粒體轉錄因子A基因(TFAM) + 綿羊��； 參考：《基因組學與應用生物學》2017年02期

【摘要】：為進一步了解和研究綿羊線粒體轉錄因子A(TFAM)基因的結構和功能,采用生物信息學方法構建了不同物種的TFAM基因系統(tǒng)發(fā)育樹,并對綿羊TFAM基因編碼蛋白質理化性質、二級結構、亞細胞定位、蛋白磷酸化、三級結構及功能結構域等進行分析。結果表明:綿羊和山羊親緣關系最為接近,其次是家牛,與原雞、斑馬魚親緣關系較遠。綿羊TFAM基因共編碼246個氨基酸,以賴氨酸含量最高;二級結構以α-螺旋和無規(guī)則卷曲占為主;綜合分析,此蛋白為堿性、不穩(wěn)定性親水蛋白,不存在信號肽和跨膜結構域;其主要在細胞核、線粒體、細胞骨架內發(fā)揮其生物學作用。該蛋白的絲氨酸磷酸化位點比較多,而酪氨酸和蘇氨酸的磷酸化位點相對較少;且含有2個HMG結構域,在兩個HMG結構域之間存在一個由12個氨基酸殘基組成的短連接區(qū)域。
[Abstract]:In order to further understand and study the structure and function of sheep mitochondrial transcription factor, TFAM gene phylogenetic tree of different species was constructed by bioinformatics, and the protein physicochemical properties and secondary structure of sheep TFAM gene were encoded. Subcellular localization, protein phosphorylation, tertiary structure and functional domains were analyzed. The results showed that the relationship between sheep and goat was the most close, followed by domestic cattle, and was far from the original chicken and zebrafish. Sheep TFAM gene encodes 246 amino acids with the highest content of lysine, and the secondary structure is mainly 偽 -helix and irregular curl, the protein is alkaline and unstable hydrophilic protein, and there is no signal peptide and transmembrane domain. It mainly plays its biological role in nucleus, mitochondria and cytoskeleton. The protein has more serine phosphorylation sites than tyrosine and threonine, and contains two HMG domains, and there is a short junction region composed of 12 amino acid residues between the two HMG domains.
【作者單位】： 河北省畜牧獸醫(yī)研究所;河北農業(yè)大學動物科技學院;
【基金】：河北省科技支撐計劃項目(15226308D)資助
【分類號】：S826;Q811.4
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本文編號：1988669