本文選題：肝 + CYPC基因�。� 參考：《畜牧獸醫(yī)學(xué)報》2016年12期

【摘要】：本研究旨在探討雞CYP2C8基因的表達調(diào)控規(guī)律。采集30周齡盧氏綠殼蛋雞心、肝、脾、肺、腎、胸肌、腹脂、肌胃、腺胃、卵巢、胰、十二指腸、空腸、回腸14種組織以及從1日齡~30周齡不同發(fā)育時期的肝組織,用熒光實時定量PCR分析CYP2C8基因的時空表達規(guī)律;用雌激素及其受體拮抗劑對活體和體外培養(yǎng)的雞胚肝原代細胞進行處理,用熒光實時定量PCR分析CYP2C8基因的表達調(diào)控機制。結(jié)果表明,雞CYP2C8基因在各組織中表現(xiàn)出廣泛表達的特性,但在脂類代謝旺盛的器官如肝、腎、小腸(十二指腸、空腸、回腸)等器官中表達較高,且在肝中的表達水平隨著性成熟而顯著升高(P0.01);雌激素處理可顯著提高雞肝組織和體外培養(yǎng)的雞胚胎肝原代細胞中CYP2C8的表達水平(P0.05),但雌激素受體ER-α拮抗劑MPP可完全抑制雌激素的作用(P0.05)。綜上表明,雞CYP2C8基因在不同組織中廣泛表達,而以肝中的表達水平最高,且肝中CYP2C8基因的表達是受雌激素并通過ER-α調(diào)控。
[Abstract]:The purpose of this study was to investigate the regulation of chicken CYP2C8 gene expression. The heart, liver, spleen, lung, kidney, pectoral muscle, abdominal fat, muscle stomach, glandular stomach, ovary, pancreas, duodenum, jejunum, ileum and liver tissues were collected from 30 week old Luhs green shell laying hens at 30 weeks of age. The temporal and spatial expression of CYP2C8 gene was analyzed by real-time quantitative PCR, estrogen and its receptor antagonists were used to treat the primary cells of chicken embryo liver in vitro and in vivo, and the regulation mechanism of CYP2C8 gene expression was analyzed by real-time quantitative PCR. The results showed that chicken CYP2C8 gene was widely expressed in various tissues, but it was highly expressed in organs with high lipid metabolism such as liver, kidney, small intestine (duodenum, jejunum, ileum) and so on. The expression level of CYP2C8 in liver was significantly increased with sexual maturation, and estrogen treatment could significantly increase the expression of CYP2C8 in chicken liver tissue and in vitro cultured chicken embryonic liver primary cells. However, estrogen receptor ER- 偽 antagonist MPP could completely increase the expression level of P0.05, and estrogen receptor ER- 偽 antagonist MPP could completely increase the expression level of P0.05G in chicken liver tissue and embryonic liver cells in vitro. Inhibitory effect of estrogen on P0.05. In conclusion, chicken CYP2C8 gene was widely expressed in different tissues, but the highest expression level was in liver, and the expression of CYP2C8 gene in liver was regulated by estrogen and ER- 偽.
【作者單位】： 河南農(nóng)業(yè)大學(xué)牧醫(yī)工程學(xué)院河南省家禽種質(zhì)資源創(chuàng)新工程研究中心河南省家禽育種國際聯(lián)合實驗室;
【基金】：河南省國際合作項目(162102410030) 地方雞種質(zhì)資源保護與利用教育部創(chuàng)新團隊發(fā)展計劃(IRT1236) 國家蛋雞產(chǎn)業(yè)技術(shù)體系遺傳育種崗位專項資金(CARS-41-K04)
【分類號】：S831

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