本文選題：豬流感病毒 + H1N1��； 參考：《吉林農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：本研究對吉林地區(qū)采集到的豬血清樣本7909份、鼻拭子及疑似發(fā)病死亡豬只的肺臟、支氣管等病料5154份,總計13063份樣本進(jìn)行了病毒分離,并且對分離到的毒株進(jìn)行亞型鑒定。結(jié)果顯示分離到的三株豬流感病毒均為H1N1亞型豬流感病毒,根據(jù)流感病毒命名標(biāo)準(zhǔn)體系分別命名為A/swine/Jilin/1-1/2011、A/swine/Jilin/1-2/2011和A/swine/Jilin/1-3/2011。對分離株HA、NA基因進(jìn)行克隆和遺傳進(jìn)化分析�；驕y序結(jié)果表明,SIV分離株A/swine/Jilin/1-1/2011(H1N1)的HA基因序列全長為1638個核苷酸,編碼區(qū)為40-1636bp,編碼532個氨基酸。NA基因序列全長為1420個核苷酸,編碼區(qū)為4-1413bp,編碼470個氨基酸;分離株A/swine/Jilin/1-2/2011(H1N1)的HA基因序列全長為1703個核苷酸,編碼區(qū)為26-1556bp,編碼510個氨基酸。NA基因序列全長為1404個核苷酸,編碼區(qū)為30-1397bp,編碼456個氨基酸;分離株A/swine/Jilin/1-3/2011(H1N1)的HA基因序列全長為1745個核苷酸,編碼區(qū)為17-1722bp,編碼568個氨基酸。NA基因序列全長為1458個核苷酸,編碼區(qū)為21-1430bp,編碼470個氨基酸。裂解位點顯示三株均為低致病性毒株,糖基化位點均未發(fā)生變異相對保守,受體結(jié)合位點顯示分離株具有感染禽源的潛力,NA基因耐藥性位點顯示這三株豬流感病毒對目前使用的神經(jīng)氨酸酶抑制劑類藥物均有效。遺傳進(jìn)化分析結(jié)果顯示A/swine/Jilin/1-1/2011和A/swine/Jilin/1-2/2011的HA和NA基因來源于野禽,與禽流感病毒的親緣關(guān)系較近。其中,A/swine/Jilin/1-1/2011和A/swine/Jilin/1-2/2011的HA基因分別與韓國2004年分離到的A/wild duck/Korean/SH60/2004的H1N1亞型流感病毒和2005年中國河北分離到的A/duck/Hebei/843/2005的H1N2亞型流感病毒親緣關(guān)系最近。同時,這兩個病毒株的NA基因都與2003年中國東部的分離到的A/duck/Eastern China/103/2003和A/duck/Eastern China/152/2003的H1N1亞型流感病毒親緣關(guān)系最近。與以上兩個病毒株明顯不同的是,A/swine/Jilin/1-3/2011的HA和NA基因則主要來自于人流感病毒和豬流感病毒,其中HA基因與中國山東、上海、廣東、廣西等地發(fā)現(xiàn)的H1N1病毒亞型親緣關(guān)系最近,NA基因與中國山東、上海、廣東等地發(fā)現(xiàn)的H1N1病毒亞型有明顯的親緣關(guān)系。
[Abstract]:In this study, 7909 pig serum samples, 5154 nasal swabs and suspected dead pigs were collected in Jilin area. A total of 13063 samples were isolated, and the subtypes of the isolated strains were identified. The results showed that the three swine flu viruses isolated were all H1N1 subtype swine flu viruses. According to the influenza virus naming standard system, they were named as A / R / J / J / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 and 1 / 3 / 1 / 1 / 1 respectively. Cloning and genetic evolution analysis of Hana gene were carried out. The result of gene sequencing showed that the HA gene sequence of Ar / Jilin / 1 / 1 / 2011H1 H1 was 1638 nucleotides, coding region was 40-1636bp. the coding length of 532 amino acids. Na gene was 1420 nucleotides, coding region was 4-1413bp, encoding 470 amino acids. The HA gene sequence of A / R / Jilin / 1 / 2 / 2011H1 / N1 was 1703 nucleotides, encoding 26-1556 BP, encoding 510 amino acids, encoding 1404 nucleotides, encoding 30-1397 BP, encoding 456 amino acids, and the HA gene sequence of A / R Jilinr / 1 / 3 / 2011H1 N _ 1 was 1745 nucleotides. The coding region is 17-1722 BP, encoding 568 amino acids. Na gene is 1458 nucleotides, encoding 21-1430 BP, encoding 470 amino acids. The cleavage sites showed that all of the three strains were low pathogenic, and the glycosylation sites were not mutated. The receptor binding sites showed that the isolates had the potential to infect avian origin. The resistance sites of na gene showed that the three swine influenza viruses were effective against the neuraminidase inhibitors currently used. Genetic evolution analysis showed that HA and na genes of A/swine/Jilin/1-1/2011 and A/swine/Jilin/1-2/2011 originated from wild birds and were closely related to avian influenza virus. Among them, A / R / J / J / 1 / 2011 and A/swine/Jilin/1-2/2011 HA genes were most closely related to A/wild duck/Korean/SH60/2004 's H1N1 subtype influenza virus isolated from Korea in 2004 and A/duck/Hebei/843/2005 's H1N2 subtype virus isolated from Hebei Province in 2005, respectively. At the same time, the na genes of the two strains were closely related to the A/duck/Eastern China/103/2003 and A/duck/Eastern China/152/2003 H1N1 subtype influenza virus isolated in eastern China in 2003. What is obviously different from the above two strains is that the HA and na genes of Ar / swine / Jilin / 1 / 3 / 2011 are mainly from human influenza viruses and swine flu viruses. Among them, HA genes are similar to those of Shandong, Shanghai, Guangdong, China. The relationship between the subtype of H1N1 virus found in Guangxi and the subtype of H1N1 virus found in Shandong, Shanghai and Guangdong provinces of China is very close to that of na gene.
【學(xué)位授予單位】：吉林農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S852.65

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本文編號：1980224