本文選題：肉雞 + 融合肽hEGF-AWRK�。� 參考：《沈陽農(nóng)業(yè)大學學報》2017年03期

【摘要】：融合肽hEGF-AWRK6(EK)是項目組在前期研究工作中利用大腸桿菌重組表達系統(tǒng),表達純化得到的融合多肽,具有人表皮生長因子hEGF和抗菌肽AWRK6的雙重功能。為探討EK對AA肉雞生產(chǎn)性能、肝臟抗氧化水平、免疫功能的影響,本研究選取84只1日齡健康AA仔肉雞,隨機分4組。對照組肉雞飼喂基礎日糧,試驗Ⅰ組肉雞飼喂添加50mg·kg~(-1)EK的基礎日糧,試驗Ⅱ組肉雞飼喂添加100mg·kg~(-1)EK的基礎日糧,抗生素組肉雞飼喂添加5mg·kg~(-1)黃霉素的基礎日糧,飼喂周期為42d。分別于試驗的21d和42d,使用丙二醛(MDA)、超化物歧化酶(SOD)、谷胱甘肽過氧化物酶(GSH-PX)、總抗氧化能力(T-AOC)和雞分泌型免疫球蛋白A(Sig-A)檢測試劑盒,測定各組肉雞相應指標的含量。結果表明:與對照組相比,飼喂添加EK的飼料顯著提高了試驗組肉雞的體重、半凈膛重與全凈膛重,提高了肉雞肝臟的SOD、GSH-PX活性水平,降低了MDA含量,同時肉雞的免疫器官指數(shù)、巨噬細胞數(shù)量、脾臟細胞增殖能力和血清中Sig-A水平也顯著提高。其中,試驗組Ⅱ肉雞的體重、半凈膛重、全凈膛重和肝臟抗氧化水平差異顯著(p0.05),免疫器官指數(shù)和巨噬細胞的增殖能力差異極顯著(p0.01)。EK對試驗組肉雞的半凈膛率和全凈堂率沒有顯著影響。融合肽EK能顯著提高肉雞的生長性能、肝臟抗氧化水平和免疫功能,具有替代飼料中抗生素的應用前景。
[Abstract]:The fusion peptide hEGF-AWRK6 (EK) is a recombinant expression system of Escherichia coli, which has dual functions of human epidermal growth factor (hEGF) and antimicrobial peptide (AWRK6). In order to study the effects of EK on the performance of AA broilers, liver antioxidant level and immune function, 84 1-day-old healthy AA broilers were randomly divided into 4 groups. The broilers in the control group were fed basic diet, the broilers in the first group were fed the basal diet supplemented with 50mg kg~(-1)EK, the broilers in the second group were fed the basic diet supplemented with 100mg kg~(-1)EK, and the broilers in the antibiotic group were fed the basal diet supplemented with 5mg KGG -1) for 42 days. Malondialdehyde (MDA) MDAA, supernatant dismutase (SOD), glutathione peroxidase (GSH-PXX), total antioxidant capacity (T-AOC) and secretory immunoglobulin A (Sig-An) were used to determine the content of corresponding indexes in broilers at 21d and 42d, respectively. The results showed that compared with the control group, the diet supplemented with EK significantly increased the body weight, half bore weight and the whole weight of the broiler, increased the activity of SOD GSH-PX in liver, decreased the content of MDA, and also increased the immune organ index of the broilers. The number of macrophages, the proliferation of spleen cells and the level of Sig-A in serum were also significantly increased. Among them, the body weight and half-bore weight of experimental group 鈪，

本文編號：1975183