本文選題：羊口瘡病毒 + 轉(zhuǎn)錄譜��； 參考：《南方醫(yī)科大學》2017年碩士論文

【摘要】：羊口瘡病毒(orfv virus;ORFV)可引起山羊和綿羊的一種急性接觸性傳染病,也可感染人、牛和其它脊椎動物,是一種自限性疾病。由于其安全性高、短期內(nèi)可反復感染并且具有強大的免疫調(diào)節(jié)活性,因此可作為一種安全有效的病毒載體用于多種感染性疾病以及腫瘤治療研究,目前已在人類腫瘤治療模型中取得初步的治療效果。然而ORFV感染人體細胞后引起宿主細胞基因的轉(zhuǎn)錄調(diào)控以及病毒與細胞之間的相互作用卻研究得較少。我們發(fā)現(xiàn)ORFV可在皮膚成纖維細胞中復制增殖并正常表達病毒蛋白,因此可通過mRNA測序技術(shù)來研究人皮膚成纖維細胞在ORFV感染后細胞及病毒基因轉(zhuǎn)錄表達水平的特異性變化,進而深入了解ORFV對人細胞的轉(zhuǎn)錄調(diào)控以及二者之間的相互作用。收集ORFV(MOI=5)感染后0、3和8小時的人皮膚成纖維細胞,抽提RNA并純化,質(zhì)檢合格(RIN9.5,28S/18S=1.6)后再進行測序文庫構(gòu)建、簇生成和上機測序。原始測序結(jié)果經(jīng)接頭、測序引物、核糖體RNA和低質(zhì)量序列的過濾后,與人基因組進行比對。我們將q值(校正后的p值)
[Abstract]:Sheep mouth sore virus orfv virus (ORFV) can cause an acute contact infectious disease in goats and sheep, but also infect humans, cattle and other vertebrates. It is a self-limited disease. Because of its high safety, reproducible infection in the short term and strong immunomodulatory activity, it can be used as a safe and effective viral vector for the study of various infectious diseases and tumor therapy. At present, the primary therapeutic effect has been obtained in human tumor treatment model. However, little research has been done on the transcriptional regulation of host cell genes and the interaction between viruses and cells caused by ORFV infection. We found that ORFV can replicate and proliferate in skin fibroblasts and normally express viral proteins, so we can study the specific changes of transcription expression level of human skin fibroblasts and viral genes after ORFV infection by mRNA sequencing. Furthermore, the transcriptional regulation of ORFV on human cells and the interaction between them are further understood. The human skin fibroblasts were collected for 0 and 8 hours after infection. RNA was extracted and purified. The sequence library was constructed and sequenced. The primordial sequencing results were compared with the human genome by the filter of the connectors, sequencing primers, ribosomal RNA and low quality sequences. We will change the Q value (the corrected p value)
【學位授予單位】：南方醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：S852.654

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本文編號：1972627