本文選題：奶牛 + SARA�。� 參考：《南京農(nóng)業(yè)大學(xué)》2015年博士論文

【摘要】：瘤胃是反芻動(dòng)物營(yíng)養(yǎng)物質(zhì)消化代謝的主要場(chǎng)所,其健康與否直接關(guān)系動(dòng)物機(jī)體健康與生產(chǎn)性能的發(fā)揮。本論文擬系統(tǒng)研究高精料日糧對(duì)奶牛瘤胃健康和泌乳性能的影響,研究?jī)?nèi)容包括以下四方面。1.SARA對(duì)瘤胃細(xì)菌菌群結(jié)構(gòu)與組成的影響試驗(yàn)選用4頭裝有瘤胃瘺管的泌乳奶牛,將其隨機(jī)分為對(duì)照組(CON,40%精料)或SARA組(SARA,70%精料),采用2×2交叉試驗(yàn)設(shè)計(jì)。每試驗(yàn)期第12、17和21天晨飼后0 h采集瘤胃液樣品,提取基因組DNA,進(jìn)行焦磷酸測(cè)序。結(jié)果顯示,SARA組奶牛瘤胃pH低于5.8的持續(xù)時(shí)間大于5小時(shí),表明SARA模型成功構(gòu)建。與對(duì)照組相比,SARA組奶牛瘤胃乳酸、丙酸、丁酸、戊酸、總VFA和LPS的濃度(P0.05)顯著升高,乙丙比(P0.05)顯著降低。SARA組奶牛瘤胃菌群豐度指數(shù)(Chao1和Ace)和多樣性指數(shù)(Shannon)顯著低于(P0.05)對(duì)照組。在門(mén)水平上,SARA組瘤胃變形菌門(mén)和擬桿菌門(mén)的相對(duì)豐度顯著降低(P0.05),但厚壁菌門(mén)和放線(xiàn)菌門(mén)的相對(duì)豐度顯著增加(P0.05)。屬水平上,與對(duì)照組相比,SARA組奶牛瘤胃中普雷沃氏菌屬、密螺旋菌屬、厭氧支原體屬、不動(dòng)桿菌屬、Papillibacter和unclassified Lentisphaerae,and unclassified bacteria的相對(duì)豐度顯著降低(P0.05),而瘤胃球菌屬、奇異菌屬、雙歧桿菌和unclassified Clostridiales的相對(duì)豐度顯著升高(P0.05)。SARA組奶牛瘤胃內(nèi)革蘭氏陰性菌的比例顯著下降,且瘤胃LPS濃度與擬桿菌門(mén)細(xì)菌拷貝數(shù)間存在顯著負(fù)相關(guān)(r =-0.446,P= 0.029)。結(jié)果說(shuō)明,高精料日糧誘發(fā)的SARA可導(dǎo)致瘤胃pH下降,VFA累積,LPS濃度升高;瘤胃細(xì)菌菌群結(jié)構(gòu)紊亂,瘤胃細(xì)菌菌群的多樣性降低。2.SARA對(duì)奶牛瘤胃代謝產(chǎn)物的影響瘤胃微生物菌群的改變與瘤胃代謝密切相關(guān),本章擬通過(guò)研究SARA對(duì)奶牛代謝產(chǎn)物的影響,進(jìn)一步解析SARA對(duì)瘤胃健康的影響。試驗(yàn)動(dòng)物、試驗(yàn)設(shè)計(jì)與試驗(yàn)日糧同上。選用每試驗(yàn)期第12、17、21天晨飼前瘤胃液樣品,進(jìn)行GC-MS代謝組學(xué)檢測(cè)。試驗(yàn)結(jié)果表明,瘤胃液樣品中共檢測(cè)到233種化合物,主要包括有機(jī)酸類(lèi)、脂肪酸類(lèi)、糖類(lèi)、氨基酸類(lèi)、嘌呤類(lèi)、胺類(lèi)、糖醇類(lèi)等物質(zhì)。統(tǒng)計(jì)顯示,較對(duì)照組相比,SARA組黃嘌呤、次黃嘌呤、尿嘧啶等細(xì)菌降解產(chǎn)物含量顯著提高(P0.05);LPS、生物胺、乙醇胺、戊二酸等有毒有害或促炎物質(zhì)的含量顯著提高(P0.05);丙氨酸、亮氨酸、甘氨酸、谷氨酸、亮氨酸等15種氨基酸的含量顯著提高(P0.05)。對(duì)差異代謝物的通路富集分析結(jié)果顯示,氨�；鵷RNA生物合成,苯丙氨酸、酪氨酸和色氨酸生物合成,以及纈氨酸、亮氨酸和異亮氨酸生物合成三條通路發(fā)生顯著富集(P0.05)。結(jié)果說(shuō)明,高精料日糧誘發(fā)的SARA會(huì)導(dǎo)致瘤胃代謝紊亂,瘤胃內(nèi)有毒或促炎物質(zhì)含量升高,氨基酸合成增加,異常代謝產(chǎn)物的增加可能會(huì)影響奶牛瘤胃和機(jī)體健康。3.SARA對(duì)瘤胃上皮基因表達(dá)的影響與機(jī)制研究本試驗(yàn)旨在通過(guò)基因芯片技術(shù)研究SARA對(duì)瘤胃上皮基因表達(dá)的影響,并結(jié)合體外細(xì)胞培養(yǎng)手段揭示瘤胃上皮炎癥相關(guān)基因?qū)Ω呔先占Z的響應(yīng)模式及其可能機(jī)制。體內(nèi)試驗(yàn):試驗(yàn)動(dòng)物、試驗(yàn)設(shè)計(jì)與試驗(yàn)日糧同上。于每期第21 d采集對(duì)照組和SARA組瘤胃上皮樣品,進(jìn)行基因芯片分析。結(jié)果顯示,兩組間共有245個(gè)基因存在差異。對(duì)差異基因進(jìn)行通路富集分析和功能注釋后,發(fā)現(xiàn)因子受體與其關(guān)聯(lián)通路(cytokine-cytokine receptor pathway)顯著富集(P0.05),通路中IL-1β、IL-2、IL-22、CCL19、CCL8、CX3CR1、CXCL6、INHBE、LEPR、PRL和TNFRSF9的表達(dá)量在SARA組奶牛瘤胃上皮中顯著上調(diào)(P0.05),而通路中IL-6和IL15RA的表達(dá)量顯著下調(diào)(P0.05),結(jié)果表明,瘤胃上皮可能已發(fā)生局部炎癥反應(yīng)。為驗(yàn)證基因芯片結(jié)果的準(zhǔn)確性,使用qRT-PCR對(duì)IL-1β、IL-2和IL-6的mRNA表達(dá)量進(jìn)行了測(cè)定。相關(guān)性分析顯示,瘤胃上皮炎癥因子與瘤胃環(huán)境因子(pH和LPS)存在關(guān)聯(lián)(P0.05),為進(jìn)一步驗(yàn)證這一結(jié)果,進(jìn)行體外細(xì)胞培養(yǎng)試驗(yàn)。結(jié)果說(shuō)明,瘤胃上皮細(xì)胞IL-1β、IL-2、IL-6和IL-8的mRNA表達(dá)量在LPS處理后顯著上調(diào)(P0.05),而低pH值處理顯著提高了瘤胃上皮細(xì)胞TNF-α的表達(dá)量,表明瘤胃環(huán)境因子pH和LPS可能在SARA引發(fā)瘤胃局部炎癥反應(yīng)過(guò)程中起著重要作用。4.SARA對(duì)奶牛泌乳性能及原奶細(xì)菌菌群的影響本章旨在研究SARA對(duì)奶牛泌乳性能及乳中細(xì)菌菌群結(jié)構(gòu)和組成的影響。試驗(yàn)動(dòng)物、試驗(yàn)設(shè)計(jì)與試驗(yàn)日糧同上。分別于每試驗(yàn)期第17、18和19天采集奶樣,并于每試驗(yàn)期第12、17和21天飼喂后0 h和4 h采集尾靜脈血,對(duì)所采集奶樣中的細(xì)菌菌群結(jié)構(gòu)和組成以及所采集血樣中的相關(guān)指標(biāo)進(jìn)行分析。結(jié)果顯示,對(duì)照組和SARA組奶牛的干物質(zhì)采食量(DMI)、產(chǎn)奶量和乳成分無(wú)顯著差異(P0.05)。較對(duì)照組相比,SARA組奶牛尾靜脈血中白細(xì)胞、淋巴細(xì)胞數(shù)量和白蛋白的含量顯著升高(P0.05),而總蛋白、球蛋白、膽固醇和低密度脂蛋白的含量顯著降低(P0.05)。454測(cè)序結(jié)果顯示,原奶中的優(yōu)勢(shì)菌群為放線(xiàn)菌門(mén)、后壁菌門(mén)、變形菌門(mén)和擬桿菌門(mén)。較對(duì)照組相比,SARA組奶牛原奶中乳房炎病原菌,包括Stenotrophomonasmaltophilia、Streptococcus parauberis、Brevundimonas diminuta 等細(xì)菌的相對(duì)豐度顯著提高(P0.05);同時(shí),SARA組奶牛原奶中嗜冷細(xì)菌,包括Pseudomonas、Brevundimonas、Sphingobacterium、Alcaligenes、腸桿菌屬和乳桿菌屬等的相對(duì)豐度也顯著提高(P0.05)。然而,兩組奶牛原奶中細(xì)菌菌群的豐度和多樣性指數(shù)(Ace,Chao和Shannon)無(wú)顯著差異。結(jié)果說(shuō)明,本試驗(yàn)中,SARA雖未顯著影響奶牛的泌乳性能,但可能影響奶牛乳腺健康,增加奶牛患乳房炎風(fēng)險(xiǎn),并可能降低原奶和乳制品的感官質(zhì)量,縮短液態(tài)奶的貯存期限。綜上所述,本論文結(jié)合組學(xué)技術(shù)層層漸進(jìn)、依次揭示了高精料飼喂誘發(fā)的SARA導(dǎo)致瘤胃細(xì)菌菌群結(jié)構(gòu)改變,群落趨于簡(jiǎn)單化;代謝紊亂,有毒有害或促炎物質(zhì)含量升高;瘤胃上皮基因表達(dá)發(fā)生改變,引發(fā)局部瘤胃炎癥;原奶中細(xì)菌菌群中乳房炎致病菌含量增多,增加奶�；既榉垦罪L(fēng)險(xiǎn)。研究結(jié)果有助于揭示瘤胃健康與宿主健康的內(nèi)在關(guān)系,為奶牛的健康飼喂提供一定的理論依據(jù)。
[Abstract]:The rumen is the main place for nutrient digestion and metabolism of ruminants, and its health is directly related to the health and production performance of the animal body. This paper intends to systematically study the effects of high concentrate diet on the ruminal health and lactating performance of dairy cows. The contents of the study include the following four aspects of the structure and composition of the bacterial flora of the rumen bacteria in the following four aspects. 4 lactating cows with rumen fistula were randomly divided into control group (CON, 40% semen) or group SARA (SARA, 70% semen), and the 2 x 2 cross test was designed. The tumor gastric juice samples were collected at 0 h for 12,17 and 21 days in each test period, and genomic DNA was extracted and sequenced. The results showed that the pH in the rumen of the SARA group was less than 5.8. The duration of the SARA model was more than 5 hours. Compared with the control group, the rumen lactic acid, propionic acid, butyric acid, valerate, valerate, total VFA and LPS concentrations (P0.05) were significantly higher in the SARA group, and the ethylene propylene ratio (P0.05) significantly decreased the abundance index of the rumen group (Chao1 and Ace) and the diversity index (Shannon) of the.SARA group significantly lower than that of the (P0.05) control group At the gate level, the relative abundance of the rumen deformable bacteria gate and the bacteriobacteria in the SARA group decreased significantly (P0.05), but the relative abundance of the actinomycetes and the actinomycetes increased significantly (P0.05). Compared with the control group, the genus Poulet Was, the genus Acinetobacter, Acinetobacter, Papillibacter and UN in the rumen of the group SARA cows. The relative abundance of classified Lentisphaerae and and unclassified bacteria decreased significantly (P0.05), while the relative abundance of rumen, kiwi, Bifidobacterium and unclassified Clostridiales increased significantly (P0.05) in the rumen of the cow's rumen of the cow, and the LPS concentration in the rumen and the bacteriobacteria were copied. There was a significant negative correlation between the number of shellfish (R =-0.446, P= 0.029). The results showed that the SARA induced by high concentrate diet could lead to the decline of the rumen pH, the accumulation of VFA, the increase of LPS concentration, the disorder of the ruminal bacterial flora and the diversity of the rumen bacterial flora, which reduced the effects of.2.SARA on ruminal microbial flora and rumen metabolism. Closely related, this chapter intends to study the effects of SARA on the metabolic products of dairy cows and further analyze the effect of SARA on the health of the rumen. Experimental animals, experimental design and experimental diet are the same. The samples of the tumor and gastric juice before 12,17,21 day morning of each test period were selected for the GC-MS metabolomics test. The results showed that 233 of the samples of the tumor gastric juice detected a total of 233 Compounds, mainly including organic acids, fatty acids, carbohydrates, amino acids, purines, amines, amines, sugar alcohols, etc. statistics show that compared with the control group, the content of the biodegradation products, such as xanthine, hypoxanthine and uracil in the SARA group, is significantly increased (P0.05); LPS, biogenic amines, ethanolamine, glutaric acid and other toxic or probiotic substances The content of 15 amino acids, such as alanine, leucine, glycine, glutamic acid, leucine, and other 15 amino acids increased significantly (P0.05). The analysis of pathway enrichment for differential metabolites showed that amyl tRNA biosynthesis, phenylalanine, tyrosine and tryptophan biosynthesis, and the biosynthesis of valine, leucine and isoleucine by valine, valine and isoleucine. The pathway was significantly enriched (P0.05). The results showed that the SARA induced by high concentrate diet could lead to the disorder of rumen metabolism, the increase of toxic or proinflammatory substances in the rumen, the increase of amino acid synthesis, and the increase of abnormal metabolites may affect the ruminal gene expression of rumen by the rumen and the body's health.3.SARA. The aim of this study was to study the effect of SARA on the gene expression in the rumen epithelium by gene chip technology, and to reveal the response patterns and possible mechanisms of ruminal dermatitis related genes to high concentrate diet by means of cell culture methods in vitro. In vivo experiments: experimental animals, experimental design and experimental diets, at the same time. A control group and SARA were collected at twenty-first D per period. The results showed that there were 245 differences between the two groups. After the pathway enrichment analysis and functional annotation, we found that the factor receptor and its associated pathway (cytokine-cytokine receptor pathway) were significantly enriched (P0.05), IL-1 beta, IL-2, IL-22, CCL19, CCL8, CX3CR1, CXCL in the pathway. 6, the expression of INHBE, LEPR, PRL and TNFRSF9 was significantly up-regulated in the rumen epithelium of SARA dairy cows (P0.05), while the expression of IL-6 and IL15RA in the pathway was significantly down (P0.05). The results showed that the rumen epithelium may have local inflammatory response. To verify the accuracy of the gene chip results, qRT-PCR is used to express the IL-1 beta, IL-2, and the expressions. The correlation analysis showed that the rumen dermatitis factor was associated with the rumen environmental factors (pH and LPS) (P0.05). In order to further verify this result, the cell culture test was carried out in vitro. The results showed that the mRNA expression of the IL-1 beta, IL-2, IL-6 and IL-8 in the rumen epithelial cells was significantly up-regulated after LPS treatment (P0.05), while the low pH value was treated significantly. The expression of TNF- alpha in the rumen epithelial cells showed that the effects of the rumen environmental factors pH and LPS on the local inflammatory response of the rumen may play an important role in the effects of.4.SARA on lactating performance and the bacterial flora of the dairy cows. This chapter aims to study the effects of SARA on milk performance and the structure and composition of bacterial flora in dairy cows. Experimental animals, experimental design and experimental diet were the same. Milk samples were collected at 17,18 and 19 days of each test period respectively, and 0 h and 4 h were collected at the 12,17 and 21 days of each trial period. The structure and composition of bacterial flora in the milk samples and the related indexes in the collected blood samples were analyzed. The results showed that the control group and the SAR were the SAR. There was no significant difference in the dry matter intake (DMI), milk production and milk composition in the A group (P0.05). Compared with the control group, the white blood cells, the number of lymphocytes and the content of albumin in the tail vein blood of the SARA group increased significantly (P0.05), while the total protein, globulin, cholesterol and low density lipoprotein were significantly reduced (P0.05).454 sequencing results. The dominant bacteria in the original milk were actinomycetes, posterior wall, deformable bacteria and bacteriobacteria. Compared with the control group, the pathogens of mastitis in the milk of SARA dairy cows, including Stenotrophomonasmaltophilia, Streptococcus parauberis, Brevundimonas diminuta and other bacteria, were significantly increased (P0.05), and the original milk of SARA Group dairy cows was also milk. The relative abundance of Pseudomonas, Brevundimonas, Sphingobacterium, Alcaligenes, Enterobacteriaceae and lactobacilli also increased significantly (P0.05). However, there was no significant difference in the abundance and diversity index (Ace, Chao and Shannon) in the two groups of dairy cows. The results showed that in this experiment, SARA did not significantly affect milk. The lactating performance of cattle may affect the health of dairy cows, increase the risk of dairy cow's mastitis, reduce the sensory quality of milk and dairy products and shorten the storage period of liquid milk. In summary, this paper, combined with the technology layer gradually, reveals the structure change of the rumen bacterial flora induced by high concentrate feeding in SARA. The community tends to be simple; metabolic disorders, toxic or harmful or proinflammatory substances increase; the gene expression in the rumen epithelium changes, causes local tumor gastritis; the bacterial flora of the bacterial flora in the original milk increases the risk of mastitis in dairy cows. The results help to reveal the inherent relationship between the health of the rumen and the health of the host, and milk for milk. The health feeding of cattle provides a certain theoretical basis.
【學(xué)位授予單位】：南京農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：S858.23

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 肖訓(xùn)軍,孟慶翔;反芻動(dòng)物瘤胃酸中毒及其調(diào)控[J];中國(guó)畜牧雜志;2001年05期

2 顧懷君,溫業(yè)嫦;洗胃導(dǎo)胃療法治療牛瘤胃酸中毒[J];貴州畜牧獸醫(yī);2001年04期

3 金朝寧;牛瘤胃酸中毒的糾正[J];青海畜牧獸醫(yī)雜志;2001年02期

4 吳元昌,張銀國(guó),李飛,何高明;一起被誤診的乳牛瘤胃酸中毒[J];畜牧與獸醫(yī);2002年10期

5 吳元昌,張銀國(guó),李飛,何高明;一起被誤診的乳牛瘤胃酸中毒[J];中國(guó)奶牛;2002年04期

6 夏兆剛,肖訓(xùn)軍;反芻動(dòng)物瘤胃酸中毒發(fā)生機(jī)理及防治的研究進(jìn)展[J];畜禽業(yè);2002年01期

7 趙慶,陳玲,葛廣山,何登峰;一起急性牛瘤胃酸中毒的治療體會(huì)[J];中國(guó)動(dòng)物檢疫;2002年04期

8 肖開(kāi)田;耕牛瘤胃酸中毒的防治[J];貴州畜牧獸醫(yī);2003年02期

9 夏季;羊喂精料過(guò)量易發(fā)生瘤胃酸中毒[J];養(yǎng)殖技術(shù)顧問(wèn);2003年03期

10 李四清;怎樣治療犢牛瘤胃酸中毒[J];河南畜牧獸醫(yī);2003年01期

相關(guān)會(huì)議論文 前10條

1 張耿;毛勝勇;姚文;朱偉云;;瘤胃酸中毒研究進(jìn)展[A];動(dòng)物生理生化學(xué)分會(huì)第八次學(xué)術(shù)會(huì)議暨全國(guó)反芻動(dòng)物營(yíng)養(yǎng)生理生化第三次學(xué)術(shù)研討會(huì)論文摘要匯編[C];2004年

2 胡寧璽;薛小蓉;;牛瘤胃酸中毒的治療[A];中國(guó)奶業(yè)協(xié)會(huì)年會(huì)論文集2009（上冊(cè)）[C];2009年

3 曹維維;王夢(mèng)芝;丁洛陽(yáng);;反芻動(dòng)物瘤胃酸中毒的發(fā)生機(jī)理及其綜合防治研究進(jìn)展[A];中國(guó)畜牧獸醫(yī)學(xué)會(huì)2010年學(xué)術(shù)年會(huì)——第二屆中國(guó)獸醫(yī)臨床大會(huì)論文集(上冊(cè))[C];2010年

4 侯全中;張勝利;;如何防治牛瘤胃酸中毒[A];冀農(nóng)杯2008“綠色奧運(yùn)”科技論文集[C];2008年

5 劉燁彤;劉大程;盧德勛;胡紅蓮;;慢性瘤胃酸中毒狀態(tài)下奶山羊瘤胃細(xì)菌內(nèi)幾種相關(guān)細(xì)菌數(shù)量變化的研究[A];中國(guó)畜牧獸醫(yī)學(xué)會(huì)動(dòng)物營(yíng)養(yǎng)學(xué)分會(huì)第十次學(xué)術(shù)研討會(huì)論文集[C];2008年

6 徐俊;;亞急性瘤胃酸中毒診斷方法研究進(jìn)展[A];第五屆中國(guó)畜牧科技論壇論文集[C];2011年

7 羅立平;賀建忠;韓博;;奶牛亞急性瘤胃酸中毒研究新進(jìn)展[A];中國(guó)畜牧獸醫(yī)學(xué)會(huì)家畜內(nèi)科學(xué)分會(huì)第七屆代表大會(huì)暨學(xué)術(shù)研討會(huì)論文集（下冊(cè)）[C];2011年

8 郭勇慶;曹志軍;李勝利;;奶牛亞急性瘤胃酸中毒診斷及發(fā)病機(jī)制研究進(jìn)展[A];中國(guó)畜牧獸醫(yī)學(xué)會(huì)養(yǎng)牛學(xué)分會(huì)2011年學(xué)術(shù)研討會(huì)論文集[C];2011年

9 謝正露;張樹(shù)坤;姜雪元;葉平生;張?jiān)词?;肝臟對(duì)高精料致亞急性瘤胃酸中毒奶山羊的保護(hù)效應(yīng)[A];全國(guó)動(dòng)物生理生化第十二次學(xué)術(shù)交流會(huì)論文摘要匯編[C];2012年

10 楊煥民;柳巨雄;欒新紅;胡仲明;;飼料應(yīng)激對(duì)肉牛HSPs表達(dá)的影響[A];中國(guó)生理學(xué)會(huì)第21屆全國(guó)代表大會(huì)暨學(xué)術(shù)會(huì)議論文摘要匯編[C];2002年

相關(guān)重要報(bào)紙文章 前10條

1 吉林省大安市 馮國(guó)明;羊瘤胃酸中毒的診斷與防治[N];河北農(nóng)民報(bào);2013年

2 孫志強(qiáng);冬春養(yǎng)羊要防瘤胃酸中毒[N];河北農(nóng)民報(bào);2013年

3 內(nèi)蒙古烏盟農(nóng)牧學(xué)校 張曉東;肉牛瘤胃酸中毒及其預(yù)防[N];中國(guó)畜牧水產(chǎn)報(bào);2000年

4 李大剛;牛瘤胃酸中毒的防治[N];中國(guó)畜牧報(bào);2004年

5 山東省沂水縣三十里獸醫(yī)站 馮培松;肉羊瘤胃酸中毒的防治[N];河北科技報(bào);2010年

6 河北遠(yuǎn)征藥業(yè) 王東軍;瘤胃酸中毒[N];中國(guó)畜牧獸醫(yī)報(bào);2007年

7 夏道倫;精料喂羊過(guò)量 謹(jǐn)防瘤胃酸中毒[N];中國(guó)畜牧報(bào);2003年

8 劉國(guó)信;羊急性瘤胃酸中毒的防治[N];中國(guó)畜牧獸醫(yī)報(bào);2005年

9 河北省畜牧獸醫(yī)研究所 安永福;瘤胃酸中毒的防控[N];河北科技報(bào);2012年

10 河北省畜牧獸醫(yī)研究所 安永福;瘤胃酸中毒的防控[N];河北科技報(bào);2012年

相關(guān)博士學(xué)位論文 前4條

1 張瑞陽(yáng);組學(xué)技術(shù)研究亞急性瘤胃酸中毒對(duì)奶牛瘤胃微生物、代謝和上皮功能的影響[D];南京農(nóng)業(yè)大學(xué);2015年

2 苑學(xué);肉牛亞急性瘤胃酸中毒微生態(tài)制劑的研制及其效果評(píng)價(jià)[D];吉林大學(xué);2011年

3 胡紅蓮;奶山羊亞急性瘤胃酸中毒營(yíng)養(yǎng)生理機(jī)制的研究[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2008年

4 李飛;奶山羊亞急性瘤胃酸中毒模型構(gòu)建與奶牛日糧CBI的優(yōu)化[D];西北農(nóng)林科技大學(xué);2014年

相關(guān)碩士學(xué)位論文 前7條

1 路丹;瘤胃異常代謝產(chǎn)物組胺對(duì)奶牛肝臟中COX-2表達(dá)的影響[D];南京農(nóng)業(yè)大學(xué);2014年

2 魏德泳;瘤胃酸中毒發(fā)生的微生物學(xué)機(jī)制及阿卡波糖調(diào)控作用的研究[D];南京農(nóng)業(yè)大學(xué);2010年

3 劉燁彤;慢性酸中毒對(duì)瘤胃發(fā)酵功能及瘤胃微生物數(shù)量變化的影響[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2009年

4 王婷婷;亞急性瘤胃酸中毒病牛瘤胃組胺對(duì)瘤胃上皮細(xì)胞炎性通路的影響[D];吉林大學(xué);2015年

5 董淑紅;硫胺素對(duì)山羊瘤胃代謝的影響及其與亞急性瘤胃酸中毒關(guān)系的研究[D];揚(yáng)州大學(xué);2011年

6 梅靈;不同產(chǎn)奶水平奶牛瘤胃酸中毒相關(guān)指標(biāo)及血液、乳、糞便中脂多糖濃度的調(diào)查研究[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2012年

7 郭立輝;VFA對(duì)亞急性瘤胃酸中毒肉牛瘤胃上皮細(xì)胞炎性信號(hào)通路的影響[D];吉林大學(xué);2015年

，

本文編號(hào)：1970565