本文選題：馬傳貧病毒 + 弱毒疫苗��； 參考：《內(nèi)蒙古農(nóng)業(yè)大學》2017年碩士論文

【摘要】：20世紀70年代,哈爾濱獸醫(yī)研究所研制成功的馬傳染性貧血病毒(Equine Infectious Anemia Virus,EIAV)弱毒疫苗為控制馬傳染性貧血(Equine Infectious Anemia,EIA)在我國的流行做出了重要的貢獻。該疫苗克服了慢病毒疫苗的開發(fā)難點,能在被免疫動物體內(nèi)產(chǎn)生堅強的保護性免疫,是迄今為止唯一廣泛應用并證明確實有效的慢病毒疫苗。為研究馬傳貧病毒(EIAV)弱毒疫苗的免疫保護機制,本研究將疫苗株EIAVDLV121免疫接種3匹馬,免疫后6個月時接種強毒株EIAVLN40,繼續(xù)觀察3個月,整個過程中對實驗馬匹體溫、血小板和血漿病毒載量進行檢測。臨床結果顯示EIAVDLV121免疫馬均未出現(xiàn)臨床癥狀,且未死亡,達到了100 保護率;而對照組未免疫馬接種等量EIAVLN40后均出現(xiàn)典型的EIA臨床癥狀,并死亡。為測定EIAV弱毒疫苗免疫馬匹后中和抗體的動態(tài)變化,本研究通過EIAV-ENV假病毒檢測系統(tǒng)測定EIAVDLV121免疫馬后6個月內(nèi)中和抗體的滴度,結果顯示隨著接種時間的增加,中和抗體的滴度也隨之升高,這提示疫苗能夠提供免疫保護可能與其誘導產(chǎn)生的中和抗體成熟相關。此外,本研究顯示未免疫馬攻毒后會產(chǎn)生以肝和脾嚴重變性、壞死為主要特征的病理變化,而疫苗免疫馬攻毒后沒有明顯病理變化;同時,對疫苗免疫馬和未免疫馬攻毒后各組織病毒載量的進行測定,結果顯示未免疫馬攻毒后組織病毒載量高達9 591 240 RNAcopy/μg,而疫苗免疫馬各組織病毒含量極低(范圍在0～148 RNA copy/μg 之間)。本研究表明疫苗能夠有效的保護機體免受強毒株的感染,而且中和抗體可能與免疫成熟、病毒載量和臨床病理變化均相關。
[Abstract]:In the 1970s, the attenuated equine Infectious Anemia virus (EIAV) vaccine developed by Harbin Veterinary Research Institute (Harbin Veterinary Research Institute) made a great contribution to the control of equine Infectious Anemiahe virus (EIAV) in China. The vaccine overcomes the difficulties in the development of lentivirus vaccine and can produce strong protective immunity in immunized animals. It is the only lentivirus vaccine that has been widely used so far and proved to be effective. In order to study the immune protection mechanism of attenuated equine-borne virus (EIAV) vaccine, three horses were inoculated with the vaccine strain EIAVDLV121, and the virulent strain EIAVLN40 was inoculated at 6 months after immunization. The body temperature of the experimental horses was observed for 3 months. Platelet and plasma viral loads were detected. The clinical results showed that none of the horses immunized with EIAVDLV121 had any clinical symptoms and no death, and the protective rate reached 100, while in the control group, the typical clinical symptoms of EIA appeared and died after inoculation of the same amount of EIAVLN40. In order to determine the dynamic changes of neutralizing antibodies in horses immunized with EIAV attenuated vaccine, the titers of neutralizing antibodies were determined by EIAV-ENV pseudovirus detection system within 6 months after inoculation with EIAVDLV121. The results showed that the titers of neutralizing antibodies increased with the increase of inoculation time. The titer of neutralizing antibody also increased, which suggested that the immune protection provided by vaccine might be related to the maturation of neutralizing antibody induced by neutralizing antibody. In addition, this study showed that unimmunized horses attack with severe degeneration and necrosis of the liver and spleen as the main characteristics of pathological changes, but vaccine inoculated horses did not have significant pathological changes; at the same time, The viral load in the tissues of vaccine-immunized and unimmunized horses was determined. The results showed that the viral load of unvaccinated horses was up to 9,591,240 RNAcopy/ 渭 g, while the viral content of vaccine-immunized horses was very low (ranging from 0 to 148 RNA copy/ 渭 g). This study shows that the vaccine can effectively protect the body from the infection of virulent strains, and neutralizing antibodies may be related to immune maturation, viral load and clinicopathological changes.
【學位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：S858.21

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本文編號：1964802