本文選題：氟苯尼考 + 鴨疫里氏桿菌��； 參考：《中國農(nóng)業(yè)科學》2017年20期

【摘要】：【目的】研究并比較肌注氟苯尼考在2周齡健康和鴨疫里氏桿菌感染鴨體內(nèi)的藥代動力學特征�！痉椒ā�240只鴨隨機分為2組,各120只鴨,其中一組用鴨疫里氏桿菌腿部感染,分別以30 mg·kg~(-1)體重單劑量肌內(nèi)注射氟苯尼考,采血點為給藥前和給藥后5、10、15、20、30、45 min和1、1.5、2、4、8、10、14 h。采用高效液相色譜法(HPLC)測定血漿氟苯尼考的濃度,采用紫外檢測器檢測,檢測波長為223 nm,流動相為乙腈和水,其體積比為26和74,流速為1 m L·min-1,色譜柱為Agilent C18(4.6 mm×150 mm,5μm),進樣量為20μL。藥動學分析軟件Win Nonlin 5.2.1以非房室模型擬合處理血藥濃度-時間數(shù)據(jù),計算相關(guān)的藥動學參數(shù),并用統(tǒng)計學軟件SPSS17.0對藥動學參數(shù)進行t檢驗統(tǒng)計學分析�！窘Y(jié)果】氟苯尼考在健康鴨體內(nèi)的峰濃度(Cmax)為(22.88±3.11)μg·m L-1,表觀分布容積(Vd/F)為(2.39±0.81)L·kg~(-1),體清除率(ClB/F)為(0.64±0.11)L·h-1·kg~(-1),消除半衰期(t1/2β)為(2.57±0.51)h和藥時曲線下面積(AUC)為(47.28±7.87)μg·m L-1·h;氟苯尼考在感染鴨體內(nèi)的峰濃度(Cmax)為(19.77±1.82)μg·m L-1,表觀分布容積(Vd/F)為(2.44±0.46)L·kg~(-1),體清除率(ClB/F)為(0.63±0.08)L·h-1·kg~(-1),消除半衰期(t1/2β)為(2.74±0.54)h和藥時曲線下面積(AUC)為(48.11±6.62)μg·m L-1·h。統(tǒng)計分析氟苯尼考在健康鴨和感染鴨的藥動學參數(shù),結(jié)果表明,除了Cmax差異顯著(P0.05),其他參數(shù)差異均不顯著(P0.05)�！窘Y(jié)論】兩者的主要藥動學參數(shù)相比較,感染鴨體內(nèi)的Cmax顯著低于(P0.05)健康鴨體內(nèi)的C_(max),其他參數(shù)無顯著性差異。氟苯尼考以30 mg·kg~(-1)體重肌內(nèi)注射在健康和感染鴨體內(nèi)具有吸收迅速,峰濃度高,體內(nèi)分布廣泛,消除較快的特點。
[Abstract]:[objective] to study and compare the pharmacokinetic characteristics of florfenicol injected intramuscularly in healthy and infected ducks at 2 weeks of age. [methods] 240 ducks were randomly divided into two groups: 120 ducks in each group. A single dose of florfenicol was injected intramuscularly with 30 mg kg 路kg ~ (-1) of body weight. The blood samples were collected for 45 min and 10 ~ 14 h before and after the administration of florfenicol at a single dose of 1.5g / kg ~ (-1) and 1.5mg / kg ~ (-1) 路L ~ (-1) 路kg ~ (-1) 路kg ~ (-1), respectively. The concentration of flufenicol in plasma was determined by high performance liquid chromatography (HPLC). The detection wavelength was 223 nm. The mobile phase consisted of acetonitrile and water. The volume ratio of acetonitrile and water was 26 and 74. The flow rate was 1 mL 路min -1. The chromatographic column was Agilent C186.mm 脳 150mm ~ (5) 渭 m ~ (-1). The amount of sample was 20 渭 L. The pharmacokinetic analysis software Win Nonlin 5.2.1 used non-atrioventricular model to deal with the plasma concentration and time data, and calculated the relevant pharmacokinetic parameters. The pharmacokinetic parameters were analyzed by SPSS17.0. [results] the peak concentration of florfenicol in healthy ducks was 22.88 鹵3.11) 渭 g mL ~ (-1), the apparent distribution volume (VD / F) was 2.39 鹵0.81L / kg ~ (-1), the body clearance rate was 0.64 鹵0.11 L 路h ~ (-1) kg ~ (-1) ~ (-1), and the elimination of half life was (t ~ (12) 尾). (2.57鹵0.51)h鍜岃嵂鏃舵洸綰夸笅闈㈢Н(AUC)涓，

本文編號：1964721