發(fā)布時間：2018-06-01 10:51

  本文選題：豬 + 解淀粉芽孢桿菌��； 參考：《中國畜牧獸醫(yī)》2017年08期

【摘要】：試驗從健康豬腸道中篩選得到一株高產(chǎn)蛋白酶的芽孢桿菌MY-6菌株,通過形態(tài)學觀察、生理生化試驗和糖醇發(fā)酵試驗、16SrDNA序列擴增與比對、Biolog鑒定等方法鑒定該菌株為解淀粉芽孢桿菌。并研究了蛋白酶活力與細菌生活周期的動態(tài)關(guān)系,pH、溫度、金屬離子和蛋白酶抑制劑對蛋白酶活力的影響及蛋白酶對不同底物的水解性能。結(jié)果顯示,MY-6菌株蛋白酶的合成與其芽孢形成同步,均開始于對數(shù)生長期的前期,該菌株所產(chǎn)蛋白酶的最適作用條件為:40℃、pH 7.0,Ca~(2+)和Mn~(2+)對蛋白酶活力有明顯的促進作用,PMSF能夠完全抑制該蛋白酶的活力,說明該蛋白酶屬絲氨酸蛋白酶,蛋白酶對魚粉蛋白、大豆蛋白、玉米蛋白、酪蛋白的酶解物TCA可溶性肽含量分別為0.83、0.67、0.58和0.38μmol/g。結(jié)果提示,解淀粉芽孢桿菌MY-6菌株可作為改善蛋白質(zhì)消化的益生性芽孢桿菌的初步選擇。
[Abstract]:A strain of Bacillus MY-6 with high protease production was obtained from the intestinal tract of healthy pigs. The 16s rDNA sequence amplification and comparison of physiological and biochemical tests and glycol-alcohol fermentation tests identified the strain as Bacillus amylolyticus by Biolog identification. The effects of pH, temperature, metal ions and protease inhibitors on protease activity and hydrolysis of different substrates were studied. The results showed that the synthesis of protease from strain MY-6 was synchronized with the formation of its spores, and both of them began at the early stage of logarithmic growth. The optimal conditions for the protease production of the strain were: 1: 40 鈩，

本文編號：1963926