本文選題：解淀粉芽孢桿菌 + 復(fù)合菌劑　； 參考：《草業(yè)學(xué)報(bào)》2017年06期

【摘要】：為獲得能提高秸稈飼草品質(zhì)的微生物發(fā)酵菌劑,本研究在前期獲得2株具有木質(zhì)素和纖維素降解能力的解淀粉芽孢桿菌菌株的基礎(chǔ)上,將兩者混配成復(fù)合菌劑,考察復(fù)合菌劑對玉米秸稈的降解作用,并通過傅立葉紅外光譜(FTIR)、核磁共振氫譜(1 H-NMR)、掃描電鏡(SEM)及氣質(zhì)聯(lián)用色譜(GC/MS)等技術(shù)對玉米秸稈的微觀結(jié)構(gòu)及降解產(chǎn)物進(jìn)行表征。研究發(fā)現(xiàn),復(fù)合菌劑可以有效降解玉米秸稈中的木質(zhì)纖維素。在發(fā)酵24d時(shí),玉米秸稈中木質(zhì)素、纖維素和半纖維素的降解率分別達(dá)到48.4%,30.5%和41.4%。FTIR和1 H-NMR譜圖中能觀察到木質(zhì)纖維素分子結(jié)構(gòu)中主要連接共價(jià)鍵,如木質(zhì)素單體間的β-O-4和β-β鍵、木質(zhì)素與碳水化合物連接鍵以及碳水化合物中糖環(huán)內(nèi)的價(jià)鍵等明顯斷裂,木質(zhì)纖維素被部分降解;SEM掃描電鏡圖則顯示發(fā)酵后秸稈的組織結(jié)構(gòu)出現(xiàn)松散和破壞。發(fā)酵后秸稈中小分子物質(zhì)的GC/MS分析結(jié)果顯示,其中包含苯丙胺和苯丙酸等保留苯丙烷結(jié)構(gòu)單元的木質(zhì)素單體衍生物以及芐醇和苯甲酸酯類等木質(zhì)素單體被進(jìn)一步降解后的芳香族化合物。玉米秸稈中碳水化合物的GC/MS分析結(jié)果表明:復(fù)合菌劑可將玉米秸稈中的結(jié)構(gòu)性多糖等大分子碳水化合物降解成葡萄糖、木糖、甘露糖及乳糖等還原性單糖。并利用這些還原性單糖生長代謝,進(jìn)一步產(chǎn)生乙二醇、丙三醇及短鏈脂肪酸類等代謝產(chǎn)物。以上研究結(jié)果表明,解淀粉芽孢桿菌復(fù)合菌劑可有效降解玉米秸稈中的木質(zhì)纖維素,在玉米秸稈飼草化利用中極具應(yīng)用前景。
[Abstract]:In order to obtain the microbial fermentation agent which can improve the quality of straw forage, 2 strains of Bacillus amylomens with lignin and cellulose degradation ability were obtained in this study, and mixed into a compound bactericide to investigate the degradation of Maize Straw by compound bacteria, and the Fu Liye infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) were used. The microstructures and degradation products of corn straw were characterized by vibration hydrogen spectrum (1 H-NMR), scanning electron microscopy (SEM) and GC-MS (GC/MS). It was found that the compound microbial agent could effectively degrade the lignocellulose in corn straw. The degradation rate of lignin, cellulose and hemicellulose in corn straw reached 48 when 24D was fermented. .4%, 30.5% and 41.4%.FTIR and 1 H-NMR spectra can observe the main covalent bonds in the lignocellulose molecular structure, such as beta -O-4 and beta bond between lignin monomers, lignin and carbohydrate bonds and the valence bonds in carbohydrates, and lignocellulose are partially degraded; SEM scanning electron microscopy shows that the lignocellulose is partially degraded. After fermentation, the structure of the straw was loosed and destroyed. After fermentation, the GC/MS analysis of the small molecular substances of straw showed that the lignin monomer, benzyl alcohol and benzoate, which contained phenylpropanamine and phenylpropanoic acid, and benzyl alcohol and benzoic acid esters were further degraded. GC/MS analysis of carbohydrates in straw shows that the compound microbial agent can degrade large molecular carbohydrates, such as structural polysaccharides in corn straw, into glucose, xylose, mannose and lactose, and make use of these reductive monosaccharide growth and metabolism to produce a further production of ethylene glycol, glycerin and short chain fatty acids. The results of the above study show that the solution of Bacillus amyloses compound bacteria can effectively degrade the lignocellulose of corn straw, and has a great application prospect in the forage utilization of corn straw.
【作者單位】： 河北農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院;
【基金】：河北省科技支撐計(jì)劃項(xiàng)目(12226605) 河北省教育廳科學(xué)技術(shù)研究項(xiàng)目(2011232)資助
【分類號】：S816

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 方躍良;;獺兔日糧中添加“633”復(fù)合菌劑飼養(yǎng)效果[J];浙江畜牧獸醫(yī);1993年03期

2 李國平,周倫江,邵良平;復(fù)合菌劑對仔豬腹瀉和生產(chǎn)性能影響的研究[J];福建畜牧獸醫(yī);1999年06期

3 胡成波;王洗清;;黃貯飼料制作技術(shù)要點(diǎn)[J];農(nóng)村百事通;2010年23期

4 薛恒平，丁洪標(biāo)，吳國俊;用復(fù)合菌劑作為添加劑飼養(yǎng)仔豬研究[J];畜牧獸醫(yī)學(xué)報(bào);1994年03期

5 侯先志,王海榮,白樺,駱麗芝;復(fù)合菌發(fā)酵劑的研制[J];內(nèi)蒙古農(nóng)牧學(xué)院學(xué)報(bào);1999年03期

6 張向陽;王曙陽;李曉明;薄永恒;梁劍平;陳積紅;劉靜;李文健;霍明德;;重離子束輻照復(fù)合菌劑發(fā)酵馬鈴薯渣的效果研究[J];甘肅農(nóng)業(yè)大學(xué)學(xué)報(bào);2013年01期

7 朱元招;尚秀國;楊立彬;張德福;王雷;樸香淑;;乳酸桿菌復(fù)合菌劑對仔豬生長和免疫的影響[J];飼料研究;2008年01期

8 王樹香;郭樂樂;李術(shù)娜;;復(fù)合菌劑發(fā)酵玉米秸稈飼料飼喂肉仔雞效果的研究[J];黑龍江畜牧獸醫(yī);2014年13期

9 陳秀為,張克強(qiáng),周可,金建;一組復(fù)合菌發(fā)酵秸稈的理化效應(yīng)及飼喂效果[J];農(nóng)業(yè)環(huán)境科學(xué)學(xué)報(bào);2004年02期

10 陶蕾;王曙陽;曹廣益;樊建華;梁劍平;;復(fù)合菌劑發(fā)酵秸稈的作用及應(yīng)用[J];甘肅農(nóng)業(yè)大學(xué)學(xué)報(bào);2011年02期

相關(guān)重要報(bào)紙文章 前1條

1 記者 王菲;新型復(fù)合菌劑“以一當(dāng)百”[N];新疆科技報(bào)(漢);2011年

相關(guān)碩士學(xué)位論文 前2條

1 殷兆麗;復(fù)合菌培養(yǎng)物高活性菌株篩選及固態(tài)培養(yǎng)基的優(yōu)化[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2010年

2 杜姍姍;生物轉(zhuǎn)化農(nóng)業(yè)副產(chǎn)物及其產(chǎn)酶研究[D];浙江大學(xué);2013年

，

本文編號：1951419