本文選題：復合益生菌 + 堿性負離子液�。� 參考：《廣西大學》2017年碩士論文

【摘要】：本試驗目的在于研究不同濃度的復合益生菌、堿性負離子液及其之間的互作效應對南丹瑤雞生長性能、肌肉品質、血清生理生化指標、肌肉重金屬含量、風味氨基酸等方面的影響。試驗研究分為三個部分:第一部分:研究在飼料中添加不同濃度的復合益生菌對16周齡的南丹瑤雞生長性能、肌肉品質、血清生理生化指標的影響。試驗采用單因素隨機設計,將240只16周齡的南丹瑤雞,隨機分成4組,每組設3個重復,每個重復20只雞。試驗預飼期1周,正飼期9周,共計10周。對復合益生菌濃度設計三個水平,分別是0.1%、0.2%、0.4%,對照組采用基礎日糧飼養(yǎng)。試驗結果表明:①生長性能:0.2%復合益生菌組在末重、平均日增重、平均日采食量、料重比方面效果最好(p0.05)。②肉品質:復合益生菌對南丹瑤雞的肉色、PH值、剪切力沒有顯著影響(p0.05),但0.1%的復合益生菌組胸肌、腿肌的系水率顯著高于其他幾組(p0.05)。③血清生理生化指標:0.2%復合益生菌顯著提高血清中TP、GLB、IgG的含量(p0.05),0.4%復合益生菌顯著降低血清中CHOL、ALT的含量(p0.05)。第二部分:研究在飲用水中添加不同濃度的堿性負離子液對16周齡的南丹瑤雞生長性能、肌肉品質、血清生理生化指標、肌肉中重金屬的殘留的影響。試驗采用單因素隨機設計,將360只16周齡的南丹瑤雞,隨機分成6組,每組設3個重復,每個重復20只雞。試驗預飼期1周,正飼期9周,共計10周。對堿性負離子液濃度設計五個水平,分別是0.2ml/L、0.25ml/L、0.33ml/L、0.5ml/L、1ml/L,對照組采用正常飲用水。試驗結果表明:①生長性能:0.5ml/L堿性負離子液組顯著提高南丹瑤雞的末重、平均日增重(p0.05),顯著降低平均日采食量、料重比(p0.05)。②肌肉品質:0.33ml/L、0.5ml/L、1ml/L濃度堿性負離子液組胸肌肉色顯著高于對照組(p0.05),試驗組腿肌剪切力高于對照組。③血清生理生化指標:0.25 ml/L堿性負離子液顯著增加血清中HDL的含量(p0.05);0.5ml/L堿性負離子液顯著降低血清中ALT、ALP的含量(p0.05),增加SOD的濃度(p0.05);1ml/L堿性負離子液顯著降低血清中COHL、LDL、GOT的含量(p0.05),增高IgM(p0.05)的含量。④肌肉中重金屬的殘留:0.25ml/L、0.33 ml/L堿性負離子液顯著降低肌肉中鉛的含量(p0.05),試驗組肌肉中未檢測到重金屬汞的含量且對降低肌肉中砷的含量有促進作用(p0.05)。第三部分:研究復合益生菌、堿性負離子液之間的互作效應對16周齡的南丹瑤雞生長性能、肌肉品質、血清生理生化指標、肌肉中風味氨基酸的影響。試驗選用360只16周齡的南丹瑤雞,采用雙因素2×3析因設計,即2個水平的復合益生菌(0.1%、0.2%)、3個水平的堿性負離子液(0.25ml/L、0.33ml/L、0.5ml/L)。隨機分成6組,每組設3個重復,每個重復20只雞。試驗預飼期1周,正飼期9周,共計10周。試驗結果表明:①生長性能:0.1%復合益生菌*0.33ml/L堿性負離子液組在末重、平均日增重、平均日采食量、料重比方面表現最好。②肌肉品質:0.1%復合益生菌*0.33ml/L堿性負離子液組顯著降低腿肌剪切力(p0.05),0.2%復合益生菌*0.25ml/L堿性負離子液組顯著提高腿肌肉色(p0.05),0.2%復合益生菌*0.5ml/L堿性負離子液顯著提高胸肌肉色。③血清生理生化指標:0.1%復合益生菌*0.33ml/L堿性負離子液組提高血清中A/G比值(p0.05),0.2%復合益生菌*0.25ml/L堿性負離子液組降低血清中膽固醇含量,0.2%復合益生菌*0.5ml/L堿性負離子液增加血清總蛋白、白蛋白的含量(p0.05)。④肌肉中風味氨基酸含量:0.1%復合益生菌*0.5ml/L堿性負離子液組對南丹瑤雞肌肉風味氨基酸的影響較大,它顯著提高肌肉中苯丙氨酸、賴氨酸、谷氨酸、酪氨酸的含量(p0.01);0.2%復合益生菌*0.25ml/L堿性負離子液組提高肌肉中甜味氨基酸絲氨酸的含量(p0.05)。
[Abstract]:The purpose of this study was to study the effects of the different concentrations of compound probiotics, alkaline negative ions and their interaction on the growth performance, muscle quality, serum physiological and biochemical indexes, heavy metal content of muscle and flavor amino acids in Nandan Yao chicken. The experimental study was divided into three parts: Part 1: the study was added to feed. The effects of the compound probiotics on the growth performance, muscle quality and serum physiological and biochemical indexes of the 16 week old Nandan Yao chicken. The trial was randomly divided into 4 groups of 4 groups of 16 weeks old Nandan Yao chicken with single factor random design. Each group had 3 repetitions and each repeated 20 chickens. The test was 1 weeks in the pre feeding period and 9 weeks in the positive feeding period. The concentration design was three levels, 0.1%, 0.2%, 0.4%, and the control group was fed with basic diet. The results showed: (1) growth performance: 0.2% compound probiotics group at the end weight, average daily weight gain, average daily feed intake, material weight ratio best (P0.05). Meat quality: Composite probiotics to Nandan Yao chicken meat color, pH, shear force did not show Influence (P0.05), but 0.1% of the complex probiotics group breast muscle, leg muscle water rate significantly higher than the other groups (P0.05). 3. Serum physiological and biochemical indicators: 0.2% compound probiotics significantly increase the serum TP, GLB, IgG content (P0.05), 0.4% compound probiotics significantly reduce the content of CHOL, ALT in blood (P0.05). Second part: Research in drinking water added The effects of different concentration of alkaline anion solution on the growth performance, muscle quality, serum physiological and biochemical indexes and the residual heavy metal in the muscle of 16 weeks old Nandan Yao chicken. The experiment was designed by single factor random design. 360 16 weeks old Nandan Yao chicken were randomly divided into 6 groups, each group was set up with 3 repetitions and 20 chickens were repeated. The test period was 1 weeks, positive. The feeding period was 9 weeks, a total of 10 weeks. The concentration of alkaline anion solution was designed five levels, which were 0.2ml/L, 0.25ml/L, 0.33ml/L, 0.5ml/L, 1ml/L. The control group used normal drinking water. The experimental results showed that: (1) growth performance: 0.5ml/L alkaline negative ionic liquid group significantly improved the final weight of Nandan Yao chicken, average daily weight gain (P0.05), and significantly reduced the average daily feed intake. The weight ratio (P0.05). (2) muscle quality: 0.33ml/L, 0.5ml/L, 1ml/L concentration of alkaline negative ion group was significantly higher than that of the control group (P0.05), and the shear force of the leg muscle in the test group was higher than that of the control group. (3) the serum physiological and biochemical indexes: 0.25 ml/L alkaline negative ionic liquid significantly increased the content of HDL in the blood (P0.05), and the 0.5ml/L basic negative ion solution was significantly reduced. The content of ALT, ALP (P0.05) in serum, increased the concentration of SOD (P0.05), and 1ml/L alkaline negative ion solution significantly reduced the content of COHL, LDL, GOT (P0.05) in the serum (P0.05), increased IgM (P0.05) content. 4. The residue of heavy metals in the muscle, 0.33 alkaline negative ionic liquid significantly reduced the content of lead in the muscle, and the weight of the muscle was not detected in the test group. The content of metal mercury can promote the content of arsenic in muscle (P0.05). The third part: study the effect of the interaction between the compound probiotics and the alkaline anion solution on the growth performance, the muscle quality, the serum physiological and biochemical index and the flavor amino acid in the muscle of the 16 weeks old Nandan Yao chicken. The experiment selected 360 16 weeks old Nan Danyao Chickens, using a double factor 2 x 3 factorial design, 2 levels of compound probiotics (0.1%, 0.2%), 3 levels of alkaline anion liquid (0.25ml/L, 0.33ml/L, 0.5ml/L), were randomly divided into 6 groups, each set of 3 repetitions, each repeat 20 chickens. The trial feeding period was 1 weeks and a total of 9 weeks in the positive feeding period. The results showed: (1) growth performance: 0.1% compound probiotic *0. 33ml/L alkaline negative ion group was at the end weight, average daily weight gain, average daily feed intake, material weight ratio, and muscle quality: 0.1% compound probiotics *0.33ml/L alkaline negative ionic liquid group significantly reduced the leg muscle shear force (P0.05), 0.2% compound probiotic alkaline negative subsolution group significantly increased leg muscle color (P0.05), 0.2% compound probiotics *0.5ml/L alkaline negative ionic liquid significantly increased the flesh color of the chest muscle. (3) the serum physiological and biochemical indexes: 0.1% compound probiotic *0.33ml/L alkaline negative ionic liquid group increased the serum A/G ratio (P0.05), the 0.2% compound probiotic *0.25ml/L alkaline negative ionic liquid group reduced the serum cholesterol content, 0.2% compound probiotics *0.5ml/L alkaline negative ionic liquid added blood. Total protein, albumin content (P0.05). (4) content of flavor amino acids in muscle: 0.1% compound probiotic *0.5ml/L alkaline anion group has great influence on the muscle flavoring amino acids of Nandan Yao chicken. It significantly improves the content of phenylalanine, lysine, glutamic acid, tyrosine (P0.01) in muscle, and 0.2% compound probiotic alkaline negative ions. The liquid group increased the content of sweet amino acid serine in muscle (P0.05).
【學位授予單位】：廣西大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：S831.5

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