本文選題：棕色田鼠 + 小鼠　； 參考：《陜西師范大學(xué)》2015年碩士論文

【摘要】：動物生存的社會環(huán)境對其生理和行為發(fā)育具有深遠的影響。社會環(huán)境對焦慮抑郁有明顯的作用,而社會支持作為一種積極地社會環(huán)境被廣泛應(yīng)用于情緒障礙的干預(yù)治療中,但社會環(huán)境影響動物焦慮抑郁的神經(jīng)內(nèi)分泌機制尚不清楚。同時,傳統(tǒng)的實驗動物如小鼠和大鼠等不具備復(fù)雜的社會行為,因此對社會支持與焦慮抑郁之間的關(guān)系及其內(nèi)在機制的研究難度較大,而社會支持等復(fù)雜社會行為卻在單配制田鼠中普遍存在,所以單配制田鼠成為社會支持神經(jīng)生物學(xué)研究的重要動物模型。棕色田鼠是一種高社會性的單配制動物,早期生存的社會環(huán)境對其成年后情緒及社會性都有影響,這為本研究提供了重要的動物模型。此外,棕色田鼠作為一種野生鼠,其生活習(xí)性、社會結(jié)構(gòu)、行為表達等均與經(jīng)典的實驗動物有較大的區(qū)別,棕色田鼠對其生存的物理及社會環(huán)境進行短期或長期適應(yīng),使其對應(yīng)激的反應(yīng)可能與小鼠和大鼠也存在一定差異�；谝陨涎芯炕A(chǔ)和假設(shè),本研究分別以小鼠和棕色田鼠作為研究對象進行比較研究,通過慢性不可預(yù)見性溫和應(yīng)激(Chronic Unpredictable Mild Stress, CUMS)誘導(dǎo)動物情緒障礙,建立應(yīng)激誘導(dǎo)情緒障礙的模型,在該模型的基礎(chǔ)上探究同胞陪伴這種社會支持在CUMS影響動物生理及情緒發(fā)育過程中發(fā)揮的緩沖效應(yīng)及其神經(jīng)機制。主要研究結(jié)果如下：1)對雄性小鼠亞成體體重增長率進行研究。結(jié)果發(fā)現(xiàn),CUMS能夠抑制動物體重的增長,而在群養(yǎng)條件下,同胞陪伴作為一種社會支持能夠有效緩沖CUMS引起的對體重增長的抑制作用。2)利用曠場實驗和高架十字迷宮實驗對動物焦慮水平進行評價。結(jié)果發(fā)現(xiàn),飼養(yǎng)模式和／或CUMS均不會改變昆明小鼠亞成體的運動性,但CUMS能夠?qū)е聠勿B(yǎng)個體出現(xiàn)焦慮傾向,而同胞陪伴能夠緩沖CUMS該效應(yīng)的發(fā)揮。3)利用強迫游泳和糖水偏好對動物抑郁水平進行檢測。結(jié)果發(fā)現(xiàn),CUMS能夠有效誘導(dǎo)其抑郁樣行為的表達,而CUMS的這一效應(yīng)可被同胞陪伴緩沖。4)對各組雄性亞成體昆明小鼠血清糖皮質(zhì)激素(Glucocorticoid, CORT)含量進行檢測。結(jié)果發(fā)現(xiàn),同胞陪伴能夠提高動物對CUMS的敏感性。5)通過免疫組織化學(xué)的方法檢測各組雄性小鼠亞成體中腦腹側(cè)被蓋區(qū)(VTA)酪氨酸羥化酶(Tyrosine Hydroxylase, TH)及海馬CA1區(qū)糖皮質(zhì)激素受體(Glucocorticorid Receptor, GR)及下丘腦室旁核(PVN)和視上核(SON)催產(chǎn)素(Oxytocin, OT)神經(jīng)元的表達。結(jié)果發(fā)現(xiàn),VTA的TH雖然與社會支持相關(guān),卻并不參與CUMS誘導(dǎo)情緒變化的過程,而海馬CA1區(qū)GR及PVN和SON的OT神經(jīng)元則參與同胞陪伴緩沖CUMS誘導(dǎo)情緒變化的過程。6)對棕色田鼠亞成體體重增長率進行比較。結(jié)果發(fā)現(xiàn),只有在無應(yīng)激情況下,同胞陪伴才能夠提高雌性的體重增長率,而在接受CUMS情況下,有無同胞陪伴,雌性棕色田鼠體重增長率都較低,說明無同胞陪伴,即單養(yǎng)(社會應(yīng)激)或CUMS單獨作用,均能使其對雌性棕色田鼠亞成體體重增長的抑制作用達到天花板效應(yīng)。而對于雄性棕色田鼠亞成體,只有既無社會支持又接受CUMS才能顯著抑制其體重增長。7)利用曠場實驗和高架十字迷宮實驗對動物焦慮水平進行評價。結(jié)果發(fā)現(xiàn),CUMS能夠?qū)е麓菩圩厣锸髞喅审w產(chǎn)生焦慮情緒,而同胞陪伴能夠緩沖CUMS該效應(yīng)的發(fā)揮。8)利用強迫游泳和糖水偏好對動物抑郁水平進行檢測。結(jié)果發(fā)現(xiàn),CUMS能夠誘導(dǎo)雄性棕色田鼠抑郁樣行為的表達,CUMS的致抑郁效應(yīng)可被同胞陪伴所緩沖。CUMS對雌性棕色田鼠亞成體則無明顯影響。綜上所述,本研究結(jié)果提示小鼠與棕色田鼠對CUMS的反應(yīng)存在種間差異,棕色田鼠對CUMS的反應(yīng)也存在明顯的性別差異,同胞陪伴作為一種社會支持對應(yīng)激誘導(dǎo)的焦慮或／和抑郁具有緩沖效應(yīng),從而降低應(yīng)激對情緒造成的負向影響,OT、多巴胺(Dopamine, DA)及CORT系統(tǒng)在此過程中具有調(diào)節(jié)作用。本研究在一定程度上揭示了社會支持改善焦慮抑郁的規(guī)律,闡明了社會環(huán)境調(diào)節(jié)焦慮抑郁的神經(jīng)內(nèi)分泌機制,并為進一步對改善動物情緒和福利的研究提供了新思路,為優(yōu)化動物的飼養(yǎng)與管理奠定了一定的理論基礎(chǔ)。
[Abstract]:The social environment of animal survival has a profound influence on its physiological and behavioral development. The social environment has an obvious effect on anxiety and depression, and social support is widely used in the intervention treatment of emotional disorders as a positive social environment. However, the neuroendocrine mechanism of the social environment affecting the anxiety and depression of animals is not yet clear. The traditional experimental animals, such as mice and rats, do not have complex social behavior, so it is difficult to study the relationship between social support and anxiety and depression, and the complex social behavior such as social support is common in the single field mice. So the single field mouse has become a social support neurobiology research. An important animal model. The brown field mouse is a highly socially single animal. The early survival of the social environment has an influence on the emotion and sociality of the adult. This provides an important animal model for this study. In addition, the brown vole, as a wild rat, is a kind of wild rat, and its living habits, social structure, behavior expression and so on are all with the classic reality. There is a big difference between animals and mice. Brown field mice have a short or long term adaptation to their physical and social environment, and the response to stress may be different from that of mice and rats. Based on the above research basis and hypothesis, the mice and brown voles were compared and studied by chronic not. Predictability and mild stress (Chronic Unpredictable Mild Stress, CUMS) induced emotional disorder in animals and the establishment of a model of stress induced emotional disorder. On the basis of this model, the buffering effect and the neural mechanism of the social support of compatriots in the process of CUMS affecting animal physiological and emotional development are explored. The results are as follows: 1) the growth rate of adult male subadult body weight was studied. The results showed that CUMS could inhibit the growth of animal body weight, and under the condition of group breeding, SIB accompany as a social support could effectively buffer the inhibitory effect of CUMS on weight growth,.2) using open field experiment and elevated cross maze test for animal anxiety water. The results showed that both the feeding pattern and / or CUMS did not change the motility of the subadult of Kunming mice, but CUMS could lead to the anxiety tendency of individual individuals, and the siblings could buffer the CUMS effect of.3) using forced swimming and sugar water preference to detect the level of depression in animals. The results found that CUMS could be used. The expression of depression like behavior was induced, and the effect of CUMS could be accompanied by sib buffering.4) to detect the content of serum glucocorticoid (Glucocorticoid, CORT) in the serum of male subadult Kunming mice. The results showed that siblings could improve the sensitivity of animal to CUMS.5) by immunohistochemical method to detect each group of males. The expression of tyrosine hydroxylase (Tyrosine Hydroxylase, TH) in the ventral ventral tegmental area (VTA) and the expression of Tyrosine Hydroxylase (GR) and the hypothalamus paraventricular nucleus (PVN) and the supraventricular nucleus (SON) of the supraventricular nucleus (Oxytocin, OT) of the hypothalamus of the middle cerebral ventral tegmental region of the sexual subadult mice Participation in the process of CUMS induced emotional changes, while the GR and PVN and SON OT neurons in the hippocampus of the hippocampal CA1 were involved in the process of sibling buffer CUMS induced mood changes.) the growth rate of the subadult body weight of the brown voles was compared. In the case of CUMS, with or without SIB, the female brown vole had a lower body weight growth rate, indicating that no SIB, that is, single breeding (social stress) or individual action, could make the female brown field mouse subadult weight increase to reach ceiling effect. For male Brown vole subadult, only neither social support nor connection. CUMS could significantly inhibit its weight gain of.7) using open field experiments and elevated cross maze tests to evaluate the level of animal anxiety. The results showed that CUMS could lead to anxiety in the adult male and female adult Brown vole subadult, while siblings could buffer the CUMS effect of.8) using forced swimming and sugar water preference for animal depression. The results showed that CUMS could induce the expression of depressive behavior in male Brown voles, and the depressive effect of CUMS was not significantly affected by the buffered.CUMS on the female brown field mouse subadult. In summary, the results suggested that there was a difference between the mice and the brown voles' response to the CUMS. There is also a significant gender difference in the response of CUMS. As a social support, compatriot as a social support has a buffer effect on induced anxiety or / and depression, thus reducing the negative impact of stress on emotion. OT, Dopamine, DA and CORT systems have a regulatory role in this process. This study reveals to some extent the society. It will support the law of improving anxiety and depression, clarifies the neuroendocrine mechanism of regulating anxiety and depression in the social environment, and provides a new idea for further research on improving animal mood and welfare, and lays a theoretical foundation for optimizing animal husbandry and management.
【學(xué)位授予單位】：陜西師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S852.3

【相似文獻】

相關(guān)會議論文 前10條

1 周奇志;蔡定均;謝川;方楊琪;余曙光;唐勇;;情緒障礙的生物鐘時間機制研究[A];2009全國時間生物醫(yī)學(xué)學(xué)術(shù)會議論文集[C];2009年

2 師見芬;;腦卒中患者致情緒障礙的觀察[A];玉溪市第十二屆內(nèi)科學(xué)術(shù)年會講義[C];2008年

3 王愛華;朱逞;張紀水;洪寶瑟;;1型糖尿病兒童情緒障礙與家長心理健康狀況關(guān)系的研究[A];中華醫(yī)學(xué)會第十四次全國兒科學(xué)術(shù)會議論文匯編[C];2006年

4 胡傳存;徐蓮英;鐘慧;張許來;;青少年情緒障礙與家庭功能關(guān)系的對照研究[A];中華醫(yī)學(xué)會第十次全國精神醫(yī)學(xué)學(xué)術(shù)會議論文匯編[C];2012年

5 張勁松;;兒童情緒障礙的診斷和治療[A];河南省兒童心理行為疾病診療實用技術(shù)培訓(xùn)班資料匯編[C];2011年

6 羅永綱;李笑燃;;試論大學(xué)生的情緒障礙及其調(diào)適[A];邁向21世紀的高校心理健康教育——第六屆全國大學(xué)生心理咨詢學(xué)術(shù)會議優(yōu)秀論文集[C];1999年

7 何艷;馬振武;李梅香;陳佐明;;120例兒童少年情緒障礙臨床分析[A];中華醫(yī)學(xué)會精神病學(xué)分會第七屆學(xué)術(shù)年會論文摘要集[C];2006年

8 王佳;陳灝;謝健;;慢性阻塞性肺疾病伴發(fā)情緒障礙患者生存質(zhì)量研究[A];2011年浙江省醫(yī)學(xué)會精神病學(xué)分會老年精神障礙學(xué)組學(xué)術(shù)會議論文匯編[C];2011年

9 章伏生;秦虹云;;燒傷患者共病情緒障礙調(diào)查[A];中華醫(yī)學(xué)會燒傷外科學(xué)分會2009年學(xué)術(shù)年會論文匯編[C];2009年

10 姚培芬;;腦梗塞后患者情緒障礙的調(diào)查[A];中國中西醫(yī)結(jié)合學(xué)會第七屆精神疾病學(xué)術(shù)討論會論文匯編[C];2002年

相關(guān)重要報紙文章 前10條

1 ;學(xué)齡前兒童打鼾可能是情緒障礙信號[N];新華每日電訊;2009年

2 山東日照 王云;偏癱愚者的情緒障礙[N];上海中醫(yī)藥報;2012年

3 魏開敏;關(guān)注兒童情緒障礙[N];人民日報海外版;2003年

4 本報記者　 湯江峰 實習(xí)生 張欽黎;湖南青少年情緒障礙發(fā)生率偏高[N];大眾衛(wèi)生報;2006年

5 和章（教授）;冬季“季節(jié)性情緒障礙”[N];上海中醫(yī)藥報;2006年

6 陳福新;形形色色的兒童情緒障礙[N];中國中醫(yī)藥報;2006年

7 朱榮申（作者為同濟大學(xué)鐵路醫(yī)院精神科主任、教授）;城市生活與情緒障礙[N];文匯報;2002年

8 ;天熱小心情緒障礙[N];大眾科技報;2005年

9 馬鳳喜;入秋防“郁悶”[N];大眾衛(wèi)生報;2009年

10 ;春天易患哪些“心”��？[N];武威日報;2008年

相關(guān)博士學(xué)位論文 前2條

1 趙兆;敘事治療在兒童情緒障礙中的應(yīng)用研究[D];南京中醫(yī)藥大學(xué);2013年

2 馮璇;中醫(yī)“心身同治”方案治療絕經(jīng)相關(guān)情緒障礙的隨機對照研究[D];廣州中醫(yī)藥大學(xué);2015年

相關(guān)碩士學(xué)位論文 前4條

1 封托;同胞陪伴對慢性不可預(yù)見性溫和應(yīng)激誘導(dǎo)的情緒障礙的緩沖效應(yīng)及其神經(jīng)機制研究[D];陜西師范大學(xué);2015年

2 陳玉;“心身同治”法治療絕經(jīng)前后情緒障礙的臨床研究[D];廣州中醫(yī)藥大學(xué);2013年

3 王昊;藥物聯(lián)合心理干預(yù)在治療伴有情緒障礙的ⅢB型前列腺炎中的應(yīng)用研究[D];山西醫(yī)科大學(xué);2014年

4 古麗扎爾·買買提明;心理干預(yù)對老年冠心病介入治療患者情緒障礙的影響[D];新疆醫(yī)科大學(xué);2006年

，

本文編號：1948069