本文選題：妊娠后期 + 宮內(nèi)生長(zhǎng)限制　； 參考：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：本實(shí)驗(yàn)旨在研究妊娠后期宮內(nèi)生長(zhǎng)限制對(duì)蒙古綿羊胎兒胸腺微環(huán)境的影響。選取同期發(fā)情受孕(B超確定懷單羔)的健康蒙古綿羊24只,當(dāng)蒙古綿羊妊娠期達(dá)到90d時(shí),挑選出與總體體重平均值相近的6只懷孕母羊解剖取胎兒及其胸腺,設(shè)為90d組,剩下的18只按體重隨機(jī)分成不同營(yíng)養(yǎng)水平組,共三組:0.18 MJME·BW-0 75·d-1(RG1:n=6)、0.33 MJME·BW-0.75·d-1(RG2:n=6)和 0.67 MJME·BW-0.75·d-1(CG:n=6),飼養(yǎng)到妊娠 140d時(shí)每組全部屠宰。試驗(yàn)結(jié)果表明:(1)妊娠后期母體受營(yíng)養(yǎng)限制,導(dǎo)致RG1組胎兒重量、胸腺重量相較于CG組均極顯著降低(p0.01);同時(shí)RG2組胎兒重量比CG組極顯著降低(p0.01),而胸腺重量則顯著低于CG組(P0.05)。隨著限制程度的加深,胸腺中的膠原纖維和網(wǎng)狀纖維逐漸增多。(2)妊娠后期RG1組胎兒胸腺中Ⅰ型膠原、纖連蛋白含量均極顯著低于CG組(P0.01),層粘連蛋白、透明質(zhì)酸含量則都顯著高于CG組(P0.05);RG2組胎兒胸腺中僅有纖連蛋白含量極顯著低于CG組(P0.01)。RG1組胎兒胸腺激素中胸腺生成素、胸腺肽β4均顯著高于CG組(P0.05);炎性因子中TNF-a(P0.01)極顯著低于CG組,IL1β、IL4均顯著低于CG組(P0.05);趨化因子中CCL25(P0.01)極顯著低于CG組,CXCL10、CXCL12、β 2微球蛋白均顯著低于CG組(P0.05);生長(zhǎng)因子中EGF、EGFR、KGF、NGF、NGFR、FGFR3 均顯著低于 CG 組(P0.05),FGFR2(P0.05)顯著高于CG組。RG2組胎兒胸腺炎性因子中TNF-a(P0.01)極顯著低于CG組,IL1 β、IL4顯著低于CG組(P0.05);趨化因子中CXCL10、CXCL12顯著低于CG組(P0.05);生長(zhǎng)因子中EGF、EGFR均顯著低于CG組(P0.05),FGFR2(P0.05)顯著高于CG組。(3)妊娠后期RG1、RG2組胎兒胸腺EMT轉(zhuǎn)化指標(biāo)中E-cad、PCDH8均顯著低于CG組(P0.05),RG1組中OB-cadherin(P0.05)顯著高于CG組。
[Abstract]:The purpose of this study was to study the effect of intrauterine growth restriction on thymus microenvironment in Mongolian sheep. Twenty-four healthy Mongolian sheep were selected for simultaneous estrus conception (B ultrasound to determine single lamb). When the gestation period of Mongolian sheep reached 90 days, 6 pregnant ewe with similar average body weight were selected to dissect the fetus and its thymus, which were divided into 90 days group. The remaining 18 rats were randomly divided into three groups according to their body weight. Three groups: 0.18 MJME BW-0 75 d ~ (-1) RG1: n ~ (1) (0.33 MJME BW-0.75 d ~ (-1) RG2: n ~ (6) and 0.67 MJME BW-0.75 d ~ (-1) CG1: n ~ (6) were raised and slaughtered in each group until day 140 gestation. The results showed that the fetal weight and thymus weight of RG1 group were significantly lower than those of CG group, and the fetal weight of RG2 group was significantly lower than that of CG group, while the weight of thymus was significantly lower than that of CG group. With the deepening of restriction, collagen fibers and reticular fibers in thymus increased gradually. (2) Type I collagen and fibronectin content in fetal thymus in RG1 group were significantly lower than those in CG group (P 0.01 and laminin). The content of hyaluronic acid in fetal thymus of CG group was significantly higher than that of CG group. Only fibronectin content in fetal thymus of CG group was significantly lower than that of CG group (P 0.01). RG1 group was significantly lower than that of CG group. Thymosin 尾 4 was significantly higher than CG group (P 0.05), inflammatory factor TNF-a (P 0.01) was significantly lower than CG group (P 0.05), chemotactic factor CCL25 (P 0.01) was significantly lower than CG group (CXCL10CXCL12), 尾 2 microglobulin was significantly lower than CG group (P 0.05), and EGFEGFRNGFNGFNGFNGFR3 in CG group was significantly lower than that in CG group (P < 0.05). FGFR2P0.05) was significantly higher than that of CG group. RG2 group (TNF-aFGFR2P0.01) was significantly lower than CG group (P 0.05); CXCL10CXCL12 was significantly lower than CG group (P0.05); and growth factor (EGFR) was significantly lower than CG group (P0.05FGFR2FGFR2 P0.05). The level of EGFR in CG group was significantly lower than that in CG group (P < 0.05); the level of CXCL10CXCL12 in CG group was significantly lower than that in CG group (P < 0.05); the level of EGFR in growth factor was significantly lower than that in CG group (P < 0.05); and that in CG group was significantly lower than that in CG group. The levels of E-cad-PCDH8 in fetal thymus of RG1 / RG2 group were significantly lower than those of CG group (P0.05 / RG1 group) and significantly higher than that of CG group (P < 0.05).
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S826

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