本文選題：豬 + 背膘厚��； 參考：《江西農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：背膘厚反映脂肪沉積能力,也是生產(chǎn)中用來度量瘦肉率的指標(biāo),因此對(duì)背膘厚的遺傳解析具有重要經(jīng)濟(jì)價(jià)值。背膘厚受品種的影響非常大,在中西方豬種之間存在顯著差異:大白、長(zhǎng)白、杜洛克等西方瘦肉型豬種背膘薄;而萊蕪、二花臉等脂肪型豬種背膘厚。隨著分子生物技術(shù)的發(fā)展以及豬60K SNP芯片的出現(xiàn),國(guó)內(nèi)外許多學(xué)者對(duì)豬的背膘厚進(jìn)行了遺傳解析,定位了許多的QTL,鑒別到一些與背膘厚關(guān)聯(lián)的SNP以及少量候選基因。這些研究主要集中在資源群體和商業(yè)群體中,對(duì)我國(guó)特有的地方豬種的研究相對(duì)較少。本研究測(cè)量了316頭萊蕪豬和334頭二花臉豬的肩部、胸部(第6至第7肋之間)、腰部和臀部的屠體背膘厚。在這兩個(gè)群體中,四個(gè)部位的背膘厚均超過25毫米,其中肩部背膘最厚,腰部背膘最薄。這兩個(gè)品種的背膘厚存在差異,萊蕪豬各部位背膘均厚于二花臉豬相應(yīng)部位的背膘。在這兩品種中,性別對(duì)背膘厚的影響也不一樣。性別對(duì)萊蕪豬的背膘厚沒有影響;而在二花臉群體中閹公豬的背膘顯著厚于母豬的背膘。另外,還計(jì)算了背膘厚、板油重、花油重和腹脂重8個(gè)脂肪沉積性狀之間的表型相關(guān)。結(jié)果顯示這些性狀之間均呈顯著正相關(guān),其中平均背膘與6-7肋膘厚的相關(guān)性最強(qiáng),相關(guān)系數(shù)達(dá)0.9以上。接下來利用豬60K SNP芯片對(duì)這650個(gè)個(gè)體進(jìn)行SNP分型,并進(jìn)行單群體的全基因組關(guān)聯(lián)分析(GWAS)以及薈萃分析(GWAS-meta)。在二花臉豬的4號(hào)和7號(hào)染色體上均檢測(cè)到與背膘厚達(dá)到基因組或染色體水平(建議水平)顯著關(guān)聯(lián)的SNP。在萊蕪豬的11號(hào)染色體上鑒別到1個(gè)與肩部膘厚關(guān)聯(lián)達(dá)染色體水平的SNP;在12號(hào)染色體上檢測(cè)到2個(gè)與胸部膘厚關(guān)聯(lián)達(dá)染色體水平的SNP。GWAS meta分析結(jié)果顯示,在4號(hào)、7號(hào)、12號(hào)和X號(hào)染色體上均檢測(cè)到與背膘厚顯著關(guān)聯(lián)的SNP。Meta分析的檢驗(yàn)效率比單群體的檢驗(yàn)效率高。除11號(hào)染色體上的信號(hào)在Meta分析中消失外,其他的染色體上的關(guān)聯(lián)在Meta分析中均可檢測(cè)到,且在X染色體上鑒別到了一個(gè)與背膘厚顯著關(guān)聯(lián)的SNP。本研究在4號(hào)、7號(hào)11號(hào)、12號(hào)和X號(hào)染色體上發(fā)現(xiàn)的關(guān)聯(lián)信號(hào)鑒證了前人的報(bào)道。為背膘厚的遺傳解析及萊蕪豬和二花臉豬背膘厚的選育提高奠定了基礎(chǔ)。
[Abstract]:Backfat thickness is an indicator of fat deposition, and it is also an indicator of lean meat rate in production. Therefore, the genetic analysis of backfat thickness is of great economic value. Backfat thickness is greatly influenced by varieties, and there are significant differences between Chinese and Western pig species: large white, long white, Duroc and other lean meat pig breeds in the west, while Laiwu, two face, etc. Fat type pigs have thick back fat. With the development of molecular biotechnology and the emergence of 60K SNP chips, many domestic and foreign scholars have inherited the genetic analysis of backfat thickness of pigs, located a lot of QTL, and identified some SNP associated with backfat thickness and a small number of candidate genes. These studies are mainly concentrated in resource and commercial groups. The study of local pig species is relatively small in our country. This study measured the shoulders of 316 Laiwu pigs and 334 two face pigs, the chest (sixth to seventh ribs), the thick back fat of the waist and buttocks. In these two groups, the back fat of four parts was more than 25 mm. The back fat of the middle shoulder was the thickest and the back fat of the waist was the thinnest. These two varieties were two varieties. The back fat of all parts of the Laiwu pig was thicker than the back fat in the corresponding part of the two Hualian pig. In these two varieties, the effect of sex on backfat thickness was different. The sex had no effect on the backfat thickness of the Laiwu pig, but the back fat of the castrate was thicker than the back fat of the sow in the two flower face population. The results showed that the correlation between the average back fat and the 6-7 ribs thickness was the strongest, and the correlation coefficient was over 0.9. Then the 650 individuals were typed by the pig 60K SNP chip, and the whole genome of the single population was carried out. Combined analysis (GWAS) and meta-analysis (GWAS-meta). On chromosome 4 and chromosome 7 of the two Hualian pigs, a significant association with the back fat thickness to the genome or chromosome level (suggested level) was detected on chromosome 11 of the Laiwu pig on chromosome 11 of Laiwu pig to identify 1 SNP associated with the shoulder fat thickness associated with the chromosome level; and 2 on chromosome 12. The SNP.GWAS meta analysis, which was associated with the chromosome level of the chest fat thickness, showed that the efficiency of the SNP.Meta analysis that was significantly associated with the back fat thickness on chromosome 4, No. 7, 12 and X was higher than that of the single population. The correlation on other chromosomes except for the signal on chromosome 11 disappeared in Meta analysis. It was detected in the Meta analysis, and on the X chromosome, a SNP. related to the back fat thickness was identified. The related signals found on chromosome 4, No. 7, No. 11, No. 12 and X were identified by previous reports. The genetic analysis for back fat thickness and the breeding and raising of the back fat thickness of Laiwu pigs and two Hualian pigs lay the foundation.
【學(xué)位授予單位】：江西農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S828

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 楊曉慧;劉源;唐輝;張寧波;武英;魏述東;姜運(yùn)良;;豬MC4R基因Asp298Asn位點(diǎn)的多態(tài)性及其與商品豬背膘厚的關(guān)系[J];農(nóng)業(yè)生物技術(shù)學(xué)報(bào);2008年03期

2 熊遠(yuǎn)著,彭中鎮(zhèn),曹勝炎,范春國(guó),陳順友,吳梅芳,汪紹南,蔡文華,胡章清;不同方法測(cè)量活豬背膘厚的準(zhǔn)確性[J];華中農(nóng)學(xué)院學(xué)報(bào);1984年02期

3 A.FORTIN;薛金良;;背膘厚與豬體各部分化學(xué)成分的關(guān)系[J];國(guó)外畜牧科技;1987年02期

4 張繼慈;;用阻抗法測(cè)定豬的背膘厚及密度推測(cè)其胴體脂肪比例[J];國(guó)外畜牧學(xué)(豬與禽);1990年05期

5 閭聯(lián)群;樊月鋼;葉樹真;;豬不同體重背膘厚的校正[J];甘肅畜牧獸醫(yī);1990年01期

6 張躍;曹紅鶴;王毓英;張代堅(jiān);趙含章;鄭友民;;豬背膘中蘋果酸脫氫酶活性與背膘厚及瘦肉率關(guān)系的初探[J];遼寧畜牧獸醫(yī);1992年06期

7 楊秀娟;鄧斌;張曦;趙金燕;王聰;葉康;陶琳麗;;豬背膘厚與眼肌厚活體A超測(cè)量技術(shù)研究[J];西北農(nóng)林科技大學(xué)學(xué)報(bào)(自然科學(xué)版);2014年05期

8 樓平兒,李學(xué)偉,朱礪;不同年齡階段活體超聲背膘厚的相關(guān)和遺傳分析[J];中國(guó)畜牧雜志;2004年03期

9 Ronnie L.Edwards;王林云;;不同背膘厚豬的胴體瘦肉測(cè)定[J];國(guó)外畜牧學(xué)(豬與禽);1984年01期

10 趙先萍;黎威;王彥玲;賀曉麗;高鵬飛;郭曉紅;李步高;曹果清;;豬鈣蛋白酶抑制蛋白基因外顯子9多態(tài)性檢測(cè)及其對(duì)山西白豬背膘厚的影響[J];中國(guó)畜牧獸醫(yī);2013年05期

相關(guān)會(huì)議論文 前10條

1 韓雪蕾;楊華威;蔣騰飛;王維民;殷勤;劉榜;;豬生長(zhǎng)和背膘厚相關(guān)基因的多基因標(biāo)記遺傳效應(yīng)研究[A];中國(guó)畜牧獸醫(yī)學(xué)會(huì)養(yǎng)豬學(xué)分會(huì)第五次全國(guó)會(huì)員代表大會(huì)暨養(yǎng)豬業(yè)創(chuàng)新發(fā)展論壇論文集[C];2010年

2 楊曉慧;劉源;張寧波;武英;姜運(yùn)良;;豬MC4R基因Asp298Asn突變的多態(tài)性及其與商品豬背膘厚的關(guān)系[A];山東畜牧獸醫(yī)學(xué)會(huì)養(yǎng)豬專業(yè)委員會(huì)第一屆學(xué)術(shù)研討會(huì)論文集[C];2007年

3 劉黎明;邱志鵬;王，

本文編號(hào)：1942400