本文選題：鴨 + CpG��； 參考：《中國(guó)家禽》2017年16期

【摘要】：為明確鴨特異性寡聚脫氧核苷酸(Cp G ODN)體外對(duì)鴨坦布蘇病毒(DTMUV)復(fù)制的抑制作用,研究在Cp G ODN最佳活性分子篩選的基礎(chǔ)上,與DTMUV感染后的細(xì)胞進(jìn)行共孵育,分別采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴鹽法(MTT法)和實(shí)時(shí)熒光定量PCR(q RT-PCR)檢測(cè)不同濃度Cp G ODN-D2對(duì)DTMUV復(fù)制的抑制作用。結(jié)果顯示:對(duì)非洲綠猴腎(Vero)細(xì)胞來(lái)說(shuō),Cp G ODN-D2濃度在300μg/m L以?xún)?nèi)均為安全濃度;MTT法和q RT-PCR檢測(cè)結(jié)果均表明,各濃度Cp G ODN-D2對(duì)DTMUV的復(fù)制均有一定的抑制作用,并隨濃度升高呈現(xiàn)先上升后下降的趨勢(shì),其中30μg/m L的濃度效果最佳,達(dá)到84.74%。結(jié)果證實(shí)了Cp G ODN-D2作為新型免疫調(diào)節(jié)劑可有效抑制DTMUV的復(fù)制。
[Abstract]:In order to determine the inhibitory effect of duck specific oligodeoxynucleotides (CPG ODN) on the replication of duck tambusuvirus (DTMUV) in vitro, we studied the co-incubation with DTMUV infected cells on the basis of screening the best active molecules of DTMUV. The inhibitory effects of different concentrations of CP G ODN-D2 on DTMUV replication were detected by using 3-trimethythiazole-2-dimethylthiazolium 5-diphenyl tetrazolium bromide method and real-time fluorescence quantitative PCR(q RT-PCR, respectively. The results showed that the concentration of CpG ODN-D2 was safe within 300 渭 g / mL and the results of Q RT-PCR assay showed that CpG ODN-D2 could inhibit the replication of DTMUV to some extent. The concentration of 30 渭 g / mL was the best, reaching 84.74. The results showed that CP G ODN-D2 could effectively inhibit the replication of DTMUV as a new immunomodulator.
【作者單位】： 山東省農(nóng)業(yè)科學(xué)院畜牧獸醫(yī)研究所山東省畜禽疫病防治與繁育重點(diǎn)實(shí)驗(yàn)室;河北工程大學(xué)生命科學(xué)與食品工程學(xué)院;
【基金】：“十三五”國(guó)家重點(diǎn)研發(fā)計(jì)劃課題(2016YFD0500106) “十二五”農(nóng)村領(lǐng)域國(guó)家科技計(jì)劃項(xiàng)目(2015BAD12B03) 山東省農(nóng)業(yè)科學(xué)院農(nóng)業(yè)科技創(chuàng)新工程(CXGC2016B14) 泰山學(xué)者特聘專(zhuān)家工程經(jīng)費(fèi)(ts201511069)
【分類(lèi)號(hào)】：S852.65

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本文編號(hào)：1938032