蛋氨酸水平對(duì)羔羊體況發(fā)育、消化道組織形態(tài)及血清抗氧化指標(biāo)的影響
發(fā)布時(shí)間:2018-05-22 12:20
本文選題:蛋氨酸 + 羔羊; 參考:《動(dòng)物營(yíng)養(yǎng)學(xué)報(bào)》2017年05期
【摘要】:本試驗(yàn)旨在研究蛋氨酸水平對(duì)羔羊體況發(fā)育、消化道組織形態(tài)及血清抗氧化指標(biāo)的影響。試驗(yàn)選取12對(duì)7日齡斷母乳的湖羊雙胞胎公羔,采用配對(duì)試驗(yàn)設(shè)計(jì),分為對(duì)照(CON)組和低蛋氨酸(LM)組,1對(duì)雙胞胎羔羊分到不同的組中。試驗(yàn)分2個(gè)階段進(jìn)行,第1階段(8~56日齡),CON組羔羊飼喂基礎(chǔ)代乳粉和基礎(chǔ)開食料;LM組羔羊飼喂的代乳粉和開食料在CON組基礎(chǔ)上分別全部扣除(0.70%和0.40%)額外添加的蛋氨酸,其余營(yíng)養(yǎng)水平保持一致。第2階段(57~84日齡),2組羔羊停止飼喂代乳粉且飼糧均為基礎(chǔ)開食料。在56和84日齡,各選取6對(duì)雙胞胎羔羊進(jìn)行屠宰,分離消化道組織,采集血清樣品。結(jié)果表明:1)在采食量方面,LM組羔羊?qū)Φ鞍彼岵墒沉吭?~56日齡、8~84日齡階段均極顯著低于CON組(P0.01),而干物質(zhì)采食量卻均顯著高于CON組(P0.05);在體尺指標(biāo)方面,56日齡,LM組除體重、體斜長(zhǎng)、胸圍和體長(zhǎng)指數(shù)顯著低于CON組(P0.05)外,其他體況發(fā)育指標(biāo)2組間均無(wú)顯著性差異(P0.05);84日齡,2組間體況發(fā)育指標(biāo)均無(wú)顯著性差異(P0.05)。2)56日齡,LM組羔羊的瘤胃乳頭寬度顯著低于CON組(P0.05),2組羔羊的其他消化道形態(tài)發(fā)育指標(biāo)差異不顯著(P0.05)。3)56日齡,LM組羔羊血清中超氧化物歧化酶活性極顯著低于CON組(P0.01);84日齡,LM組羔羊血清中除谷胱甘肽過氧化物酶活性顯著低于CON組(P0.05)外,2組羔羊的其他血清抗氧化指標(biāo)差異均不顯著(P0.05)。結(jié)果提示,飼糧低蛋氨酸水平抑制了羔羊體況(體重、體斜長(zhǎng)、胸圍、體長(zhǎng)指數(shù))發(fā)育及瘤胃乳頭寬度的增加,同時(shí)降低了機(jī)體血清中超氧化物歧化酶活性,然而卻提高了干物質(zhì)采食量;提高飼糧蛋氨酸水平后,羔羊體況發(fā)育及消化道組織形態(tài)發(fā)育也隨之得到補(bǔ)償,但機(jī)體抗氧化防御系統(tǒng)仍未得到完全改善。
[Abstract]:The purpose of this experiment was to study the effect of methionine on the development of the lamb, the morphology of the digestive tract and the serum antioxidant index. 12 pairs of lambs with 7 day old breastfeeding were selected and divided into the control (CON) group and the low methionine group (LM), and 1 pairs of twin lambs were divided into different groups. The experiment was divided into 2 stages. In the first stage (8~56 days of age), group CON lambs were fed with basic milk powder and basal diet feed. The milk powder and feeding material fed by LM group were all deducted (0.70% and 0.40%) added methionine on the basis of group CON, and the rest nutrition level remained the same. The second stage (57~84 days of age), 2 groups of lambs stopped feeding milk powder and feed all At the age of 56 and 84, 6 pairs of twin lambs were slaughtered, the digestive tract was separated and the serum samples were collected. The results showed that 1) in the feed intake, the LM lambs were significantly lower than the CON group (P0.01) at the age of 8~56 and the 8~84 days of age (P0.01), while the dry matter intake was significantly higher than that of the CON group (P0.05). In terms of body scale index, 56 days of age, group LM except body weight, body length, chest circumference and body length index were significantly lower than group CON (P0.05), and there was no significant difference between the other 2 groups of body condition development indicators (P0.05); 84 days old, there was no significant difference between the 2 groups (P0.05).2) 56 days old, the ruminal papilla width of the LM group was significantly lower than that of the CON group (P0.05), 2 The other digestive tract morphological development indexes of the group lambs were not significant (P0.05).3) 56 days old. The activity of superoxide dismutase in the serum of LM group was significantly lower than that of the CON group (P0.01). The activity of glutathione peroxidase in the serum of LM group was significantly lower than that of the CON group (P0.05), and the difference of other serum antioxidant indexes of the 2 lambs was all in the group of 84 days of age. It was not significant (P0.05). The results suggested that the level of low methionine in diet inhibited the growth of lamb body condition (body weight, body length, chest circumference, body length index) and the increase of the width of the rumen nipple, while reducing the activity of superoxide dismutase in the body of the body, but increased the feed intake of the dry matter; after raising the level of dietary methionine, the body condition of the lamb was developed and The development of alimentary canal has been compensated, but the antioxidant defense system has not been completely improved.
【作者單位】: 中國(guó)農(nóng)業(yè)科學(xué)院飼料研究所農(nóng)業(yè)部飼料生物技術(shù)重點(diǎn)開放實(shí)驗(yàn)室;
【基金】:國(guó)家公益性行業(yè)(農(nóng)業(yè))科研專項(xiàng)(201303143) 國(guó)家肉羊產(chǎn)業(yè)技術(shù)體系建設(shè)專項(xiàng)(CARS-39)
【分類號(hào)】:S826.5
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