吡啶羧酸鉻對蛋鴨生長性能及生理生化指標的影響
本文選題:鉻 + 蛋鴨。 參考:《東北農(nóng)業(yè)大學(xué)》2015年碩士論文
【摘要】:本試驗旨在研究飼糧中不同的鉻的添加水平對5-10周齡籠養(yǎng)蛋鴨生長性能及生理生化指標的影響,并對差異顯著性指標進行回歸分析,通過回歸方程確定鉻(吡啶羧酸鉻)的添加量。試驗選取健康、體重接近的28日齡金定蛋鴨180只,依據(jù)單因素完全隨機分組設(shè)計方法,將試驗用鴨隨機分為5個處理組(I~V組),每組6個重復(fù),每個重復(fù)6只試鴨。試驗采用玉米—豆粕型基礎(chǔ)飼糧,對照組(I組)試鴨飼喂基礎(chǔ)飼糧,試驗組(II~V組)分別在基礎(chǔ)飼糧中添加0.15 mg/Kg、0.30 mg/Kg、0.45 mg/Kg、0.60 mg/Kg鉻(吡啶羧酸鉻)。試驗期為6周(5-10周齡)。在整個試驗過程中,每兩周稱一次體重并記錄相應(yīng)的耗料量,計算每兩周的平均日增重、平均日采食量及料重比。試驗結(jié)束時,從每個重復(fù)中選取一只與本重復(fù)中平均體重接近的試鴨,稱活重并做好記錄。然后從其頸靜脈采血為測定生化指標備用。取血后將鴨屠宰,分別取其胸腺、脾臟、法氏囊和肝臟等器官待測。生長試驗結(jié)果:飼糧中添加0.30 mg/Kg的鉻(吡啶羧酸鉻)能顯著提高生長期籠養(yǎng)蛋鴨的平均日增重(P0.05),但對其料重比和日采食量無顯著影響(P0.05)。隨著鉻添加水平的增加,日增重表現(xiàn)出先增加后降低的趨勢。適宜鉻添加水平對生長期籠養(yǎng)蛋鴨產(chǎn)生了一定影響,過高或過低均不能發(fā)揮其最佳的生長性能。免疫器官指數(shù):飼糧中添加0.30 mg/Kg的鉻(吡啶羧酸鉻)顯著提高了生長期籠養(yǎng)蛋鴨的胸腺指數(shù)(P0.05),對脾臟指數(shù)和法氏囊指數(shù)無顯著影響(P0.05)?寡趸笜:飼糧中添加0.30 mg/Kg的鉻(吡啶羧酸鉻)可顯著提高肝臟中總抗氧化能力(T-AOC)(P0.05),對血清中總抗氧化能力(T-AOC)影響不顯著(P0.05),顯著提高了肝臟和血清中總超氧化物歧化酶(T-SOD)和血清中谷胱甘肽過氧化物酶(GSH-PX)的活性(P0.05),血清中丙二醛(MDA)的含量也顯著性降低(P0.05)。血清生化指標:飼糧中添加0.30 mg/Kg的鉻(吡啶羧酸鉻)顯著提高了血清中葡萄糖(GLU)、總蛋白(TP)、白蛋白(ALB)的含量(P0.05),血清中總膽固醇(TC)、甘油三酯(TG)的含量顯著降低(P0.05)。血清中尿素氮(BUN)的含量顯著降低(P0.05),當飼糧中鉻的添加量為0.15 mg/Kg時,其含量最低。血清免疫指標:飼糧中添加0.30 mg/Kg的鉻(吡啶羧酸鉻)顯著提高了血清中Ig G、Ig M和IL-2的含量(P0.05),但對Ig A未產(chǎn)生顯著影響(P0.05)。血清激素指標:飼糧中添加0.30 mg/Kg的鉻(吡啶羧酸鉻)可顯著提高血清中生長激素(GH)含量(P0.05),但對胰島素(Ins)含量影響不顯著(P0.05)。通過統(tǒng)計最終試驗數(shù)據(jù),從機體的生長、免疫、血清生化指標和抗氧化指標等方面分析,可以得出如下結(jié)論,給生長期籠養(yǎng)蛋鴨飼喂添加適量鉻(吡啶羧酸鉻)的玉米—豆粕型基礎(chǔ)飼糧可提高蛋鴨的日增重、免疫和抗氧化等功能,對其生長產(chǎn)生了一定的影響。針對顯著性指標進行回歸分析,通過二次曲線回歸方程計算,最終得出鉻(吡啶羧酸鉻)的適宜添加量為0.297-0.423 mg/Kg。
[Abstract]:The purpose of this study was to study the effects of different chromium supplementation levels on the growth performance and physiological and biochemical indexes of 5-10 week old cage ducks, and to make regression analysis on the significant difference indexes. The addition of chromium (chromium picolinate) was determined by regression equation. 180 Jinding ducks of 28 days old, which were healthy and close to weight, were selected in the experiment. According to the method of single factor complete random grouping design, the ducks were randomly divided into 5 treatment groups, I. e. V group, 6 replicates in each group and 6 ducks in each replica. The experimental ducks were fed with corn-soybean meal basal diet and control group I, respectively. The experimental group was fed with 0.15mg / kg / kg 0.45mg / kg ~ (0.45) mg / kg ~ (0.60) mg/Kg of chromium pyridine carboxylate in the basal diet, and the experimental group was fed with a basal diet of 0.15 mg / kg ~ (0.30) mg / kg ~ (0.45) mg 路kg ~ (-1) 路g ~ (-1) 路kg ~ (-1), respectively. The experiment period was 6 weeks and 5 to 10 weeks old. During the whole experiment, the weight was weighed once every two weeks and the corresponding feed consumption was recorded. The average daily gain, average daily feed intake and feed weight ratio were calculated every two weeks. At the end of the experiment, one duck with the same average weight as the average weight of the replica was selected from each replica, and the live weight was weighed and recorded. Then the blood collected from its jugular vein was used for the determination of biochemical indexes. The ducks were slaughtered and their thymus, spleen, bursa of Fabricius and liver were collected. The results of growth test showed that the average daily gain (ADG) of caged duck was significantly increased by adding 0.30 mg/Kg chromium to the diet, but there was no significant effect on the feed weight ratio and daily feed intake. With the increase of chromium addition level, daily gain showed a trend of first increasing and then decreasing. The suitable level of chromium supplementation had a certain effect on the growing stage of caged duck, and too high or too low could not give full play to its best growth performance. Immune organ index: the thymus index (P0.05) was significantly increased by adding 0.30 mg/Kg Cr (Chromium pyridyl) to diet, but had no significant effect on spleen index and bursa index of bursa of Fabricius. Antioxidant index: chromium (chromium pyridine carboxylate) added in diet for 0. 30 mg/Kg could significantly increase the total antioxidant capacity of liver (TAOC0. 05), but had no significant effect on the total antioxidant capacity of serum (T- AOC0. 05), and significantly increased the total superoxide in liver and serum. The activity of T-SOD and GSH-PX in serum and the content of malondialdehyde (MDAs) in serum also decreased significantly. Serum biochemical indexes: dietary chromium (Cr) of 0.30 mg/Kg significantly increased the contents of GLU, TP, ALB in serum, and decreased the contents of TCU and TGG in serum. The content of bun (bun) in serum decreased significantly (P 0.05), and the content of Cr was the lowest when the amount of Cr added in diet was 0. 15 mg/Kg. Serum immunological index: chromium (chromium picolinate) supplemented with 0.30 mg/Kg in diet significantly increased the contents of Ig GG M and IL-2 in serum, but had no significant effect on IgA. Serum hormone index: chromium (chromium pyridine carboxylate) added to diet for 0. 30 mg/Kg could significantly increase serum growth hormone (GH) content (P 0. 05%), but had no significant effect on insulin content (P 0. 05%). By analyzing the final test data from the aspects of body growth, immunity, serum biochemical index and antioxidant index, we can draw the following conclusions. The basic corn-soybean meal diet supplemented with proper amount of chromium (chromium pyridyl) in growing cage laying duck could improve the function of daily weight gain, immunity and antioxidation, and had a certain effect on the growth of laying duck. Based on the regression analysis of significant indexes, the optimum addition amount of chromium (chromium pyridyl) is 0.297-0.423 mg / kg through the quadratic regression equation.
【學(xué)位授予單位】:東北農(nóng)業(yè)大學(xué)
【學(xué)位級別】:碩士
【學(xué)位授予年份】:2015
【分類號】:S834.5
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