發(fā)布時間：2018-05-21 10:16

  本文選題：嘉興黑豬 + 雌激素受體　； 參考：《浙江農(nóng)林大學(xué)》2015年碩士論文

【摘要】：豬繁殖性狀被公認(rèn)為是低遺傳力的數(shù)量性狀和經(jīng)濟(jì)性狀,中國地方豬種在這一性狀表現(xiàn)比較優(yōu)良。在豬遺傳育種過程中,傳統(tǒng)雜交育種和分子標(biāo)記輔助選擇育種相結(jié)合(marker-assisted selection,MAS)成為現(xiàn)代選種、保種技術(shù)的新方向。本研究中以嘉興黑豬為研究對象,選取雌激素受體(Estrogen Receptor,ESR)基因、卵泡刺激素β亞基(Follicle-Stimulating Hormoneβ,FSHβ)基因、催乳素受體(Prolactin Receptor,PRLR)基因?yàn)楹蜻x基因,采取513份嘉興黑豬耳組織為試驗(yàn)材料,抽提其基因組DNA,以候選基因的外顯子區(qū)域設(shè)計(jì)引物,PCR擴(kuò)增得到目的序列,目的片段采用PCR-SSCP方法和直接測序方法檢測SNPs,統(tǒng)計(jì)分析各基因的等位基因頻率和基因型頻率、Hardy-Weinberg平衡情況以及其與繁殖性狀關(guān)聯(lián)分析結(jié)果,為建立嘉興黑豬的分子標(biāo)記輔助育種模型奠定基礎(chǔ)。ESR基因第一外顯子區(qū)域擴(kuò)增目的片段,測序后分析得到4個SNPs:T6901C、G6759A、T6927G、A7188G。測序峰圖表明每個SNP處都出現(xiàn)三種基因型,分別命名為AA、AB、BB、NN、NM、MM、EE、EF、FF、JJ、HJ和HH。統(tǒng)計(jì)分析了這些位點(diǎn)的基因型頻率和基因頻率,卡方檢驗(yàn)表明,T6901C、A7188G兩個基因座均處于Hardy-Weinberg平衡狀態(tài),G6759A、T6927G顯著偏離Hardy-Weinberg平衡狀態(tài)。選取嘉興黑豬產(chǎn)仔數(shù)、活仔數(shù)、死胎數(shù)、胎間距、初生均重5個繁殖相關(guān)性狀與多態(tài)性位點(diǎn)T6901C、A7188G的六種基因型分別進(jìn)行性狀關(guān)聯(lián)分析結(jié)果表明,T6901C位點(diǎn)與性狀關(guān)聯(lián)分析三個基因型之間無顯著性差異;而A7188G位點(diǎn)中HH基因型個體產(chǎn)仔數(shù)與活仔數(shù)均顯著高于JJ基因型個體(P0.05),死胎數(shù)性狀HH基因型顯著高于JJ基因型(P0.05),初生重性狀HH性狀顯著高于JJ基因型(P0.05),在其他性狀,各基因型之間無顯著性差異。FSHβ基因的外顯子2上擴(kuò)增的目的片段測序分析獲得到3個SNPs:T1035C、A922C、A925G。每個SNP位點(diǎn)都出現(xiàn)三種基因型,分別命名為KK、KL、LL、QQ、QR、RR、UU、UV、VV。統(tǒng)計(jì)分析了這些位點(diǎn)的基因頻率和基因型頻率,卡方檢驗(yàn)表明,A922C位點(diǎn)顯著偏離Hardy-Weinberg平衡狀態(tài),T1035C、A925G位點(diǎn)符合Hardy-Weinberg平衡狀態(tài)。T1035C、A925G位點(diǎn)各基因型與嘉興黑豬群體5個繁殖性狀關(guān)聯(lián)分析,結(jié)果顯示T1035C位點(diǎn)各基因型之間無顯著性差異,G925A位點(diǎn)分析發(fā)現(xiàn),UU基因型與VV基因型個體的初生重顯著高于UV基因型(P0.05)。PRLR基因第5外顯子區(qū)域發(fā)現(xiàn)1個SNPs:A13902T,PCR-SSCP檢測顯示存在三種基因型:XX、XY和YY。統(tǒng)計(jì)分析了這些位點(diǎn)的基因頻率和基因型頻率,卡方檢驗(yàn)表明,此位點(diǎn)符合Hardy-Weinberg平衡狀態(tài);與5個繁殖性狀關(guān)聯(lián)分析結(jié)果顯示,產(chǎn)仔數(shù)性狀、活仔數(shù)性狀、初生重性狀在A13902T位點(diǎn)的基因型之間差異顯著(P0.01)XXXYYY,與其他性狀各基因型之間則無顯著性差異。A7188G位點(diǎn)與A13902T兩個位點(diǎn)合并基因型效應(yīng)對于五個繁殖性狀關(guān)聯(lián)分析發(fā)現(xiàn),合并基因型中,產(chǎn)仔數(shù)性狀HHXX基因型比JJXX基因型每胎多產(chǎn)2.03頭(P0.05),HJXX基因型比JJXY基因型每胎多產(chǎn)2.12頭(P0.05),比JJYY基因型每胎多產(chǎn)2.38頭(P0.05)。對于活仔數(shù)性狀HHXX基因型比JJXX基因型每胎多1.95頭(P0.05),比JJYY基因型多3.53頭(P0.03),初生重性狀HHXX基因型每窩比JJYY基因型重4.37kg(P0.05),比JJXY基因型每窩重3.83kg(P0.05)。死胎數(shù)性狀與胎間距性狀沒有顯著影響。
[Abstract]:The reproductive traits of pigs are considered to be quantitative and economic traits of low heritability, and Chinese local pigs have excellent performance in this character. In the process of swine genetics and breeding, the combination of traditional hybrid breeding and molecular marker assisted selection breeding (marker-assisted selection, MAS) has become a modern selection and the new direction of seed conservation. Taking the Jiaxing black pig as the research object, we selected the estrogen receptor (Estrogen Receptor, ESR) gene, the follicle stimulating hormone beta subunit (Follicle-Stimulating Hormone beta, FSH beta) gene, the prolactin receptor (Prolactin Receptor, PRLR) gene as the candidate gene, and took 513 copies of the Jiaxing black pig ear tissue as the test material, and extracted its genome DNA, which was a candidate base. The exons were designed with primers, and the target sequence was amplified by PCR. The target fragment was detected by PCR-SSCP method and direct sequencing method for SNPs. The allele frequency and genotype frequency of each gene, Hardy-Weinberg balance and its correlation with reproductive traits were analyzed to assist the establishment of molecular markers in Jiaxing black pig. The breeding model laid the base of the first exon of.ESR gene to amplify the target fragment. After sequencing, 4 SNPs:T6901C, G6759A, T6927G, and A7188G. sequencing peak maps showed that there were three genotypes at each SNP, which were named AA, AB, BB, NN, NM. Rate, chi square test showed that the two loci of T6901C and A7188G were all in the state of Hardy-Weinberg balance, G6759A and T6927G deviated from the equilibrium state of Hardy-Weinberg significantly. The number of litter size, the number of live births, the number of stillbirths, the distance between the fetus, and the primary weight of the Jiaxing black pigs were all 5 reproductive correlation and polymorphic loci T6901C, and the six genotypes of A7188G were respectively carried out. The correlation analysis showed that there was no significant difference between the three genotypes of the T6901C loci and the traits, while the number of litter and the live number of the HH genotype in the A7188G locus were significantly higher than that of the JJ genotype (P0.05). The HH genotype of the number of stillbirths was significantly higher than that of the JJ genotype (P0.05), and the HH traits of the primary weight traits were significantly higher than the JJ genotype (P). 0.05), in other traits, there was no significant difference between the genotypes, and the sequence analysis of exon 2 of.FSH beta gene was sequenced. Three genotypes were found at 3 SNPs:T1035C, A922C, and A925G. at each SNP site, named KK, KL, LL, QQ, QR, RR, UU, and genotype frequencies. The chi square test showed that the A922C locus was significantly deviated from the equilibrium state of Hardy-Weinberg, T1035C and A925G were in accordance with Hardy-Weinberg equilibrium state.T1035C, and the genotype of A925G loci was associated with 5 reproductive traits in Jiaxing black pig population. The results showed no significant difference between the genotypes of the T1035C loci, the G925A site analysis found, the UU genotypes and the genotype of the.T1035C. The primary weight of the VV genotype was significantly higher than that of the UV genotype (P0.05).PRLR gene fifth exon region found 1 SNPs:A13902T, PCR-SSCP detection showed three genotypes: XX, XY and YY. statistical analysis of the gene frequency and genotype frequency of these loci, chi square test showed that this site was in line with Hardy-Weinberg equilibrium state; and 5 propagation. The results of the correlation analysis showed that the number of litter, the number of live litter, and the genotypes of the primary weight traits were significantly different (P0.01) XXXYYY, and there was no significant difference between the genotypes of the other traits and the other traits. The combination of the genotype effects of the.A7188G and A13902T two sites was found to be found in the association analysis of the two reproductive traits. In the genotype, the number of HHXX genotypes was 2.03 more (P0.05) than that of the JJXX genotype, and the HJXX genotype was 2.12 more (P0.05) per fetus than the JJXY genotype, and 2.38 more (P0.05) more than the JJYY genotype per tire. The HHXX genotype of the living litter was 1.95 more (P0.05) than the JJXX genotype, and 3.53 more than the JJYY genotype. The weight per litter of HHXX genotype was more than JJYY genotype 4.37kg (P0.05), and 3.83kg (P0.05) per litter weight of JJXY genotypes. There was no significant effect on the number of stillbirths and fetal distance traits.
【學(xué)位授予單位】：浙江農(nóng)林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S828

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 楊洪雁;胡銳;王星;白秀娟;;日本大白兔PRLR基因外顯子10的克隆與測序[J];江蘇農(nóng)業(yè)科學(xué);2010年01期

2 ;Analysis of PRLR and BF Genotypes Associated with Litter Size in Beijing Black Pig Population[J];Agricultural Sciences in China;2008年11期

3 張冬杰;沙偉;楊國偉;劉娣;;催乳素受體基因(PRLR)與產(chǎn)仔數(shù)關(guān)系的研究[J];安徽農(nóng)業(yè)科學(xué);2005年12期

4 曹果清;李步高;石建中;劉宏;劉建華;周忠孝;;豬PRLR基因多態(tài)性及其與產(chǎn)仔性能的關(guān)聯(lián)分析[J];畜牧獸醫(yī)學(xué)報;2009年04期

5 阮征,王春芳,韓艷云,凌家瑞,鐘思華,葉成風(fēng);以催乳素受體基因(PRLR)作為母豬產(chǎn)仔數(shù)候選基因的分析[J];上海畜牧獸醫(yī)通訊;2002年01期

6 朱盼;岳理;耿照玉;姜潤深;袁紹友;夏生林;;鵝PRLR基因外顯子10單核苷酸多態(tài)性分析[J];河北農(nóng)業(yè)科學(xué);2009年03期

7 王懷禹;;催乳素受體(PRLR)基因多態(tài)性與動物繁殖性能的關(guān)系[J];黑龍江動物繁殖;2009年03期

8 王懷禹;;PRLR基因多態(tài)性與動物繁殖性能的關(guān)系[J];中國農(nóng)學(xué)通報;2009年12期

9 張向楠;孫偉;倪蓉;左其生;王蘭萍;張有法;陳玲;吳文忠;周洪;;湖羊PRLR基因遺傳多樣性及其與母性行為的關(guān)聯(lián)分析[J];中國畜牧雜志;2013年17期

10 陳從英;朱萬成;李平華;魏娜;郭源梅;高軍;;白色杜洛克×二花臉F_2資源群體中催乳素(PRL)和催乳素受體(PRLR)基因與母豬殺嬰行為和產(chǎn)仔數(shù)關(guān)聯(lián)性研究[J];中國農(nóng)業(yè)科學(xué);2010年11期

相關(guān)會議論文 前10條

1 王杰;侯水生;劉小林;黃葦;;北京鴨PRLR基因部分序列的克隆及分析[A];2009中國肉鴨產(chǎn)業(yè)發(fā)展高層論壇論文集[C];2009年

2 陳從英;朱萬成;郭源梅;黃路生;;PRL、PRLR基因在白色杜洛克×二花臉資源群體中遺傳變異及其與產(chǎn)仔數(shù)關(guān)聯(lián)性分析[A];中國動物遺傳育種研究進(jìn)展——第十五次全國動物遺傳育種學(xué)術(shù)討論會論文集[C];2009年

3 吳洪賓;王遵寶;余鵬;趙宗勝;;PRLR基因外顯子10多態(tài)性及其與綿羊產(chǎn)羔數(shù)關(guān)系的研究[A];第十二次全國畜禽遺傳標(biāo)記研討會論文集[C];2010年

4 黃賀;狄生偉;田亞光;李和平;王希彪;;民豬生殖器官PRLR基因表達(dá)發(fā)育變化的研究[A];中國畜牧獸醫(yī)學(xué)會動物繁殖學(xué)分會第十五屆學(xué)術(shù)研討會論文集（上冊）[C];2010年

5 王宵燕;李碧春;經(jīng)榮斌;宋成義;;RBP_4和PRLR基因與蘇姜豬產(chǎn)仔性狀的相關(guān)性分析[A];中國動物遺傳育種研究進(jìn)展——第十五次全國動物遺傳育種學(xué)術(shù)討論會論文集[C];2009年

6 楊雨江;徐日福;徐慧;包艷波;秦寧;王志賢;姜建萍;田曉東;;鴨PRL和PRLR基因多態(tài)位點(diǎn)及聚合基因型與其產(chǎn)蛋性狀間的關(guān)系分析[A];第十二次全國畜禽遺傳標(biāo)記研討會論文集[C];2010年

7 陳杰;劉卉玲;楊帆;王燕;李平;蔡亞非;劉紅林;王根林;;二元雜交系皖江白鵝催乳素(PRL)、催乳素受體基因(PRLR)的多態(tài)性與產(chǎn)蛋量的相關(guān)性研究[A];第十二次全國畜禽遺傳標(biāo)記研討會論文集[C];2010年

8 褚曉紅;胡錦平;盧立志;徐寧迎;;七個鵝種PRLR基因的單核苷酸多態(tài)性研究[A];第十二次全國畜禽遺傳標(biāo)記研討會論文集[C];2010年

9 胡雪松;王希彪;;民豬、長白豬及其雜種母豬催乳素受體(PRLR)基因的PCR-RFLPs分析[A];中國畜牧獸醫(yī)學(xué)會2004學(xué)術(shù)年會暨第五屆全國畜牧獸醫(yī)青年科技工作者學(xué)術(shù)研討會論文集（上冊）[C];2004年

10 張巧靈;姜俊兵;賀俊平;赫曉燕;耿建軍;董常生;;催乳素受體基因(PRLR)與羊駝繁殖性能關(guān)系的初探[A];中國畜牧獸醫(yī)學(xué)會動物解剖學(xué)及組織胚胎學(xué)分會第十四次學(xué)術(shù)研討會論文集[C];2006年

相關(guān)博士學(xué)位論文 前1條

1 曹果清;豬PRLR基因和FSHβ亞基基因多態(tài)性研究[D];山西農(nóng)業(yè)大學(xué);2004年

相關(guān)碩士學(xué)位論文 前10條

1 王華鑫;[D];陜西師范大學(xué);2015年

2 李進(jìn)寬;奶牛STAT5A、PRL、PRLR及GHR基因相關(guān)miRNAs的初步研究[D];西北農(nóng)林科技大學(xué);2015年

3 常新俠;濟(jì)寧青山羊生后乳腺中GHR、IGF-IR、INSR和PRLR的分布和表達(dá)的發(fā)育性變化[D];山東農(nóng)業(yè)大學(xué);2015年

4 趙強(qiáng);綿羊PRLR,PRLH和PRLHR基因的克隆及其在下丘腦—垂體—性腺中表達(dá)的研究[D];甘肅農(nóng)業(yè)大學(xué);2015年

5 李曉林;湖羊PRL、PRLR基因與泌乳性狀相關(guān)性分析[D];石河子大學(xué);2015年

6 張潔;全轉(zhuǎn)錄組分析△S2 LF-PRLR對人類乳腺癌細(xì)胞（MCF-7）基因表達(dá)的影響[D];吉首大學(xué);2015年

7 鐔忠斌;嘉興黑豬繁殖性狀相關(guān)基因ESR、FSHβ、PRLR多態(tài)性及其關(guān)聯(lián)分析研究[D];浙江農(nóng)林大學(xué);2015年

8 宋永利;牛PR及PRLR基因多態(tài)性及其與精液品質(zhì)的相關(guān)性分析[D];吉林大學(xué);2009年

9 李小平;PRL、PRLR基因?qū)δ肛i多產(chǎn)性影響的研究[D];湖南農(nóng)業(yè)大學(xué);2004年

10 汪峰;雞PRL、PRLR基因表達(dá)及其與繁殖性能的關(guān)系[D];南京農(nóng)業(yè)大學(xué);2007年

，

本文編號：1918793