發(fā)布時(shí)間：2018-05-18 04:03

  本文選題：雞源大腸桿菌 + 致病性��； 參考：《中國獸醫(yī)學(xué)報(bào)》2017年09期

【摘要】：選取來自山東等10省698株雞源大腸桿菌為研究對象,分別利用PCR、微量肉湯稀釋法、MLST技術(shù)方法進(jìn)行系統(tǒng)進(jìn)化群分群、耐藥表型檢測、分子分型,并采用Bio Numerics v4.0軟件和SPSS20.0軟件進(jìn)行數(shù)據(jù)處理分析。結(jié)果顯示,低致病群B_1群為48.85%(341/698)和非致病群A群24.64%(172/698)分布較多,高致病群D群20.20%(141/698)和B_2群為6.31%(44/698)。雞源大腸桿菌耐藥嚴(yán)重,12種藥物的耐藥率約在50%以上。B_2和D群菌株對8種抗菌藥的耐藥率高于A和B_1群菌株,其中對慶大霉素、大觀霉素、頭孢噻呋、氨芐西林和奧格門丁5種藥物極顯著性差異(P0.01),對氟苯尼考和復(fù)方新諾明顯著性差異(P0.05)。135株高致病菌株經(jīng)MLST分型得到48個(gè)ST型,ST117(12株)和ST354(12株)最多,其次是ST115(8株),ST2309(8株),ST10型(7株),ST69(6株),ST1011(5株),ST1158(5株)。有29個(gè)ST型各1株,18株菌株未分出序列型。不同地區(qū)大腸桿菌ST型分布存在差異,ST69和ST354型分布最為廣泛(4個(gè)省),其次是ST115、ST117和ST2309型(3個(gè)省),ST10、ST48、ST57、ST362、ST371、ST1011和ST1158(2個(gè)省),其余36個(gè)ST型菌株僅在1個(gè)省市有檢出。135株菌株分為35個(gè)聚類簇,其中23個(gè)聚類簇的遺傳相似性在40%以下,但有6個(gè)聚類簇菌株間的遺傳相似性均在80%以上。48個(gè)ST型菌株共獲得了76種耐藥譜。我國各地健康雞群隱性攜帶相對較高比例的高致病菌株,且耐藥程度相對嚴(yán)重,耐藥譜較廣。雞源大腸桿菌菌株分布具有多態(tài)性和地域性,ST型與耐藥譜之間無相關(guān)性。
[Abstract]:In this study, 698 strains of Escherichia coli from 10 provinces in Shandong Province were selected as the research objects. The methods of PCR and MLST were used to detect the phenotype of resistance and molecular typing. Bio Numerics v4.0 software and SPSS20.0 software are used for data processing and analysis. The results showed that the distribution of low pathogenic group B _ (1) was 48.85 / 691 / 698) and that of non-pathogenic group A was 24.64 / 172 / 698). The distribution of high pathogenic group D was 20.20141/ 698) and that of B2 was 6.31 / 698. The resistance rate of 12 strains of Escherichia coli was more than 50%. The resistance rates of strains D and B to 8 antibiotics were higher than those of strains A and B 1, among which gentamicin, spectinomycin and cefotaxime were resistant to gentamicin, spectinomycin and cefotaxime. There were significant differences between ampicillin and Ogementin (P 0.01), and there were significant differences between p florfenicol and compound neutrol (P 0.05. 135) strains of high pathogenic bacteria were obtained by MLST typing, and 48 ST-ST117N (12 strains) and ST354(12 strains were the most. The next was ST115(8 strain ST2309 (8 strains) and ST10 type (7 strains). There were 29 ST-type 1 strain each and 18 strains were not sequenced. ST69 and ST354 were the most widely distributed in different regions (4 provinces, followed by ST115 ST117 and ST2309 (3 provinces), ST1010 ST48, ST57, ST372T371T1011 and ST1158. the remaining 36 ST-type strains were found in 35 clusters in only one province. The genetic similarity of 23 cluster strains was below 40%, but the genetic similarity among 6 cluster strains was above 80%. A total of 76 drug resistance profiles were obtained from 48 ST-type strains. There is a relatively high proportion of high pathogenic bacteria in recessive chicken populations in China, and the drug resistance degree is relatively serious and the spectrum of drug resistance is relatively wide. The distribution of Escherichia coli strains from chicken had no correlation with drug resistance.
【作者單位】： 中國動(dòng)物衛(wèi)生與流行病學(xué)中心;青島農(nóng)業(yè)大學(xué)動(dòng)物科技學(xué)院;青島機(jī)場出入境檢驗(yàn)檢疫局;
【基金】：國家“十二五”科技基礎(chǔ)性工作專項(xiàng)資助項(xiàng)目(2012FY111000) 農(nóng)業(yè)部“引進(jìn)國際先進(jìn)農(nóng)業(yè)科學(xué)技術(shù)”重點(diǎn)項(xiàng)目“獸藥監(jiān)管技術(shù)引進(jìn)”資助項(xiàng)目(2011-G14)
【分類號(hào)】：S852.612

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本文編號(hào)：1904274