本文選題：犬 + 子宮蓄膿　； 參考：《揚州大學(xué)》2015年碩士論文

【摘要】：子宮蓄膿是臨床上常見的中老年犬的產(chǎn)科疾病,不僅對生殖系統(tǒng)造成損傷,還對病犬其他器官造成危害,嚴(yán)重時可引起病犬死亡。該研究病例來源于2012～2014年在揚州大學(xué)動物醫(yī)院就診犬,其中閉合型子宮蓄膿15例,開放型子宮蓄膿23例。本文以施行絕育術(shù)的18例健康母犬作為對照組,對子宮蓄膿病例的臨床資料(發(fā)病年齡、品種、初次就診體溫、發(fā)病季節(jié)、發(fā)病距離最后一次發(fā)情結(jié)束間隔、生育史等)、血液學(xué)指標(biāo)、性激素變化規(guī)律、子宮與卵巢的病理組織學(xué)變化和子宮與卵巢相關(guān)基因和蛋白的表達(dá)進(jìn)行研究。研究的結(jié)果如下：子宮蓄膿的平均發(fā)病年齡為8.63±2.95歲,7歲以上發(fā)病率高達(dá)84.21%,其中博美犬和京巴犬為本試驗中的高發(fā)品種,發(fā)病率分別為23.68%和18.42%；閉合型子宮蓄膿和開放型子宮蓄膿初次就診體溫分別為39.07±0.99℃和39.03±0.81℃；大部分病例發(fā)病是在發(fā)情之后2個月內(nèi)：有生育史的犬占總發(fā)病數(shù)的21.11%,明顯低于無生育史犬的比例。血液指標(biāo)和激素檢測結(jié)果表明：閉合型子宮蓄膿組的血液白細(xì)胞、單核細(xì)胞、嗜中性粒細(xì)胞和淋巴細(xì)胞的數(shù)量高于對照組,且差異極顯著(P0.01)。閉合型和開放型子宮蓄膿組的血液平均紅細(xì)胞容積低于對照組,且差異顯著(P0.05)。閉合型和開放型子宮蓄膿組的血液平均血小板容積低于對照組,且極差異顯著(P0.01)。外周血及子宮血血清中,雌激素不論在外周血清還是子宮血清中都表現(xiàn)為閉合組最高、開放組次之,對照組最低,且在子宮血清中,閉合組極顯著高于對照組(P0.01)。孕酮在外周血清及子宮血清中的變化相似,閉合組均顯著高于對照組(P0.05)。子宮蓄膿犬子宮與卵巢的病理組織學(xué)變化：子宮蓄膿多伴隨CHE的發(fā)生,子宮內(nèi)膜固有層增厚,子宮腺體增大擴(kuò)張,當(dāng)腺體極度擴(kuò)張時,腺體上皮破壞,腺體內(nèi)充滿白細(xì)胞及蛋白。子宮內(nèi)膜上皮周圍聚集大量白細(xì)胞,嚴(yán)重者出現(xiàn)上皮壞死、脫落,有時可見子宮肌層血管充血擴(kuò)張。蛋白檢測發(fā)現(xiàn)：健康犬卵巢、子宮體肌層ER的表達(dá)強(qiáng)度較低：子宮蓄膿犬在子宮體內(nèi)膜及子宮角腺體基質(zhì)的表達(dá)升高。健康犬子宮角和子宮體肌層及腺體PR表達(dá)強(qiáng)度較低,卵巢及子宮體內(nèi)膜表達(dá)較高。TLR2及TLR4在子宮蓄膿犬子宮體、子宮角及卵巢中表達(dá)均高于健康犬,但差異不顯著。基因表達(dá)檢測分析表明：與對照組相比,TLR2基因在患開放型子宮蓄膿犬卵巢組織中的表達(dá)量顯著升高(P0.05),而在閉合型子宮蓄膿犬則極顯著升高(P0.01)；在子宮角組織中,閉合組極顯著高于對照組(P0.01)。TLR4基因在閉合型子宮蓄膿犬的子宮角及卵巢中的表達(dá)量均高于對照組,差異極顯著(P0.01)；在卵巢組織中,開放型子宮蓄膿犬的表達(dá)量較對照組顯著升高(P0.05)。ER-α基因的表達(dá)量,在開放型子宮蓄膿犬的子宮角組織中,顯著高于對照組(P0.05),而閉合型子宮蓄膿犬極顯著高于對照組(P0.01)。PR基因的表達(dá)量,在開放型子宮蓄膿犬的子宮角組織中,顯著高于對照組(P0.05),而閉合型子宮蓄膿犬極顯著高于對照組(P0.01)。結(jié)論：臨床上,犬子宮蓄膿可引起病犬血液WBC數(shù)量、雌二醇及孕酮水平的升高。子宮內(nèi)膜的病理變化以CHE為主,卵巢為持久黃體或卵巢囊腫。TLR2、TLR4、ER、PR蛋白陽性表達(dá)及基因的相對表達(dá)量在兩組子宮蓄膿犬生殖器官中均有不同程度的升高。
[Abstract]:Uterine sepsis is a clinically common obstetric disease of the middle-aged and old dog. It not only causes damage to the reproductive system, but also causes harm to other organs of the sick dog. It can cause the death of the sick dog in serious case. The case of this study was found in 2012~2014 years in the animal hospital of Yangzhou University, including 15 cases of closed uterine purulent and 23 cases of open type of uterus. The clinical data (age, variety, body temperature, season, distance of the last estrus, birth history, etc.), Hematology Index, sex hormone change law, histopathological changes of uterus and ovary, and uterus and egg of uterus and ovum were used in 18 healthy female dogs as control group. The results of the expression of nests related genes and proteins are studied. The results of the study are as follows: the average age of the onset of uterine sepsis is 8.63 + 2.95 years, and the incidence of the disease is up to 84.21% above the age of 7. The BBO and Beijing Ba dogs are high incidence in this test, with the incidence of 23.68% and 18.42%, respectively. The body temperature was 39.07 + 0.99 C and 39.03 + 0.81 C respectively. Most cases occurred within 2 months after the estrus: the dogs with a history of birth accounted for 21.11% of the total incidence, obviously lower than the proportion of the non bearing history dogs. The blood indexes and hormone test results showed that the blood leukocytes, monocytes and neutrophils in the closed uterine purulent group The number of cells and lymphocytes was significantly higher than that in the control group (P0.01). The blood average blood volume of the closed and open uterine purulent groups was lower than that of the control group, and the difference was significant (P0.05). The blood average platelet volume in closed and open uterine purulent groups was significantly lower than that in the control group (P0.01). In the blood serum of the uterus, estrogen was the highest in the peripheral blood serum and in the uterus serum, the open group and the control group were the lowest, and in the uterine sera, the closed group was significantly higher than the control group (P0.01). The changes of progesterone in the peripheral serum and the uterus serum were similar, the closed group were significantly higher than the control group (P0.05). The uterus was significantly higher than the control group. The histopathological changes in the uterus and ovary of the purulent dog: the uterine sepsis is associated with the occurrence of CHE, the thickening of the inner lamina of the endometrium, the enlargement of the uterus gland, the destruction of the gland epithelium and the white cells and protein in the gland when the gland is extremely dilated. The expression of ER in the healthy canine ovary and the myometrium of the uterus was lower, and the expression of the endometrium and the uterine horn gland matrix in the uterus increased. The expression of the uterus angle and the myometrium and the gland PR in the healthy dog was lower, the expression of the ovary and the endometrium was higher.TLR2. The expression of TLR4 in the uterine body, uterus angle and ovary of the uterus was higher than that of the healthy dog, but the difference was not significant. The analysis of gene expression analysis showed that compared with the control group, the expression of TLR2 gene in the ovary tissue of the open type uterine purulent dog was significantly higher (P0.05), while in the closed uterine purulent dog (P0.01); In the uterine horn tissue, the closed group was significantly higher than the control group (P0.01).TLR4 gene expression in the uterus angle and ovary of the closed uterine purulent dog was higher than the control group, the difference was very significant (P0.01); in the ovarian tissue, the expression of the open type uterus purulent dog increased significantly (P0.05) the expression of.ER- alpha gene, in the opening of the ovary. The uterus angle tissue of the canine type uterine septic was significantly higher than that of the control group (P0.05), while the closed uterine purulent dog was significantly higher than the control group (P0.01).PR gene expression, significantly higher than the control group (P0.05) in the uterus angle tissue of the open type uterus purulent dog (P0.05), and the closed uterine purulent dog was significantly higher than that of the control group (P0.01). In clinical, the Canine Uterus purulent can cause the number of WBC, the level of estradiol and progesterone. The pathological changes of the endometrium are mainly CHE, the ovary is the persistent corpus luteum or ovarian cyst.TLR2, the TLR4, ER, PR protein positive expression and the relative expression of the gene in the two groups of uterus purulent canine reproductive organs have different degrees of increase.

【學(xué)位授予單位】：揚州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S858.292

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本文編號：1893559